中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
16期
1262-1264
,共3页
银屑病%角蛋白细胞%抗菌肽类%S100A8%钙泊三醇
銀屑病%角蛋白細胞%抗菌肽類%S100A8%鈣泊三醇
은설병%각단백세포%항균태류%S100A8%개박삼순
Psoriasis%Keratinocytes%Dermcidins%S100A8%Calcipotriol
目的 探讨钙泊三醇对人角质形成细胞抗菌肽S100A8基因表达的影响.方法 体外培养的HaCaT细胞,分为空白对照组、加入100 ng/ml肿瘤坏死因子α(TNF-α)的TNF-α组、加入10-5 ～ 10-9 mol/L钙泊三醇的钙泊三醇组、TNF-α与钙泊三醇共同刺激的TNF-α+钙泊三醇组,所有细胞共同孵育24 h.实时定量PCR法检测并计算S100A8 mRNA相对表达量.结果 100 ng/mlTNF-α处理HaCaT细胞后24 h,S100A8 mRNA表达较空白对照上调(19.623 ±3.486)倍(P＜0.01);10-7、10-8 mol/L钙泊三醇处理细胞24 h后,S100A8表达分别较空白对照上调(5.029±1.056)、(2.848 ±0.612)倍(均P＜0.01).10-5 mol/L钙泊三醇与TNF-α共同处理细胞后,S100A8表达下调为单独使用TNF-α时的(59.51±3.31)％(P＜0.05);10-7 mol/L钙泊三醇与TNF-α共同处理细胞后,S100A8表达较单独使用TNF-α时上调(1.873 ±0.153)倍(P＜0.01).结论 TNF-α可以诱导体外培养的角质形成细胞高度表达S100A8.钙泊三醇双向调节角质形成细胞S100A8表达:高浓度(10-5 mol/L)钙泊三醇下调S100A8表达,低浓度(10-7～10-8mol/L)钙泊三醇上调S100A8表达.
目的 探討鈣泊三醇對人角質形成細胞抗菌肽S100A8基因錶達的影響.方法 體外培養的HaCaT細胞,分為空白對照組、加入100 ng/ml腫瘤壞死因子α(TNF-α)的TNF-α組、加入10-5 ～ 10-9 mol/L鈣泊三醇的鈣泊三醇組、TNF-α與鈣泊三醇共同刺激的TNF-α+鈣泊三醇組,所有細胞共同孵育24 h.實時定量PCR法檢測併計算S100A8 mRNA相對錶達量.結果 100 ng/mlTNF-α處理HaCaT細胞後24 h,S100A8 mRNA錶達較空白對照上調(19.623 ±3.486)倍(P＜0.01);10-7、10-8 mol/L鈣泊三醇處理細胞24 h後,S100A8錶達分彆較空白對照上調(5.029±1.056)、(2.848 ±0.612)倍(均P＜0.01).10-5 mol/L鈣泊三醇與TNF-α共同處理細胞後,S100A8錶達下調為單獨使用TNF-α時的(59.51±3.31)％(P＜0.05);10-7 mol/L鈣泊三醇與TNF-α共同處理細胞後,S100A8錶達較單獨使用TNF-α時上調(1.873 ±0.153)倍(P＜0.01).結論 TNF-α可以誘導體外培養的角質形成細胞高度錶達S100A8.鈣泊三醇雙嚮調節角質形成細胞S100A8錶達:高濃度(10-5 mol/L)鈣泊三醇下調S100A8錶達,低濃度(10-7～10-8mol/L)鈣泊三醇上調S100A8錶達.
목적 탐토개박삼순대인각질형성세포항균태S100A8기인표체적영향.방법 체외배양적HaCaT세포,분위공백대조조、가입100 ng/ml종류배사인자α(TNF-α)적TNF-α조、가입10-5 ～ 10-9 mol/L개박삼순적개박삼순조、TNF-α여개박삼순공동자격적TNF-α+개박삼순조,소유세포공동부육24 h.실시정량PCR법검측병계산S100A8 mRNA상대표체량.결과 100 ng/mlTNF-α처리HaCaT세포후24 h,S100A8 mRNA표체교공백대조상조(19.623 ±3.486)배(P＜0.01);10-7、10-8 mol/L개박삼순처리세포24 h후,S100A8표체분별교공백대조상조(5.029±1.056)、(2.848 ±0.612)배(균P＜0.01).10-5 mol/L개박삼순여TNF-α공동처리세포후,S100A8표체하조위단독사용TNF-α시적(59.51±3.31)％(P＜0.05);10-7 mol/L개박삼순여TNF-α공동처리세포후,S100A8표체교단독사용TNF-α시상조(1.873 ±0.153)배(P＜0.01).결론 TNF-α가이유도체외배양적각질형성세포고도표체S100A8.개박삼순쌍향조절각질형성세포S100A8표체:고농도(10-5 mol/L)개박삼순하조S100A8표체,저농도(10-7～10-8mol/L)개박삼순상조S100A8표체.
Objective To explore the effects of calcipotriol on the expression of S100A8 in human keratinocytes.Methods Cultured HaCaT cells were divided into 4 groups of blank without interventions,tumor necrosis factor-alpha (TNF-α) 24 h stimulation with 100 ng/ml TNF-α,calcipotriol 24 h stimulation with 10-5-10-9 mol/L calcipotriol and calcipotriol + TNF-α 24 h stimulation.The relative expression of S100A8 mRNA was detected and calculated by real-time quantitative polymerase chain reaction (PCR).Results The relative expression of S100A8 mRNA was up-regulated to (19.623 ± 3.486) folds (P ＜ 0.01) under a 24 h stimulation of 100 ng/ml TNF-α versus blank.The expression of S100A8 was up-regulated to (5.029 ± 1.056) and (2.848 ±0.612) folds (both P ＜0.01) when cultured for 24 h with 10-7,10 8 mol/L calcipotriol versus blank respectively.And the expression of S100A8 was down-regulated to (59.51 ± 3.31)％ (P ＜ 0.05) when cultured with 10-5 mol/L calcipotriol + TNF-α versus TNF-α-stimulated cells.The expression of S100A8 was up-regulated to (1.873 ± 0.153) folds (P ＜ 0.01) when cultured with 10-7 mol/L calcipotriol + TNF-α versus TNF-α-stimulated cells.Conclusions TNF-αinduces a high expression of S100A8 in cultured human keratinocytes in vitro.Calcipotriol bi-directionally affects the expression of S100A8:A high concentration (10-5 mol/L) calcipotriol down-regulates while a low concentration (10-7-10-8 mol/L) calcipotriol up-regulates the expression of S100A8.
