中华实验和临床病毒学杂志
中華實驗和臨床病毒學雜誌
중화실험화림상병독학잡지
CHINESE JOURNAL OF EXPERIMENTAL AND CLINICAL VIROLOGY
2015年
2期
103-105
,共3页
胡艳芳%王淼%史为涛%王勤英
鬍豔芳%王淼%史為濤%王勤英
호염방%왕묘%사위도%왕근영
癌,肝细胞%肝炎病毒,乙型%基因%突变
癌,肝細胞%肝炎病毒,乙型%基因%突變
암,간세포%간염병독,을형%기인%돌변
Carcinoma,hepatocellular%Hepatitis B virus%Genes%Mutation
目的 探讨乙型肝炎病毒(HBV)前C区基因变异与肝细胞癌(HCC)发生的关系.方法 收集在山西医科大学第一医院感染病科门诊及住院的患者血清及病例资料共99例,其中慢乙肝55例,肝癌44例.采用半巢式聚合酶连反应及直接测序法检测前C区基因变异的情况.分别检测两组患者的乙肝病毒标志物、HBV DNA,采用病例对照研究方法对患者的年龄、性别、HBeAg、HBVDNA基线水平和HBV前C区基因变异位点进行分析.结果 (1)肝癌患者的HBeAg阴性率均高于慢乙肝组,差异有统计学意义(P＜0.05).(2)肝癌组患者HBV DNA载量低于慢乙肝组,差异有统计学意义(P＜0.05).(3)共发现前C区有G1896A、G1899A两个热点突变位点,其中G1896A位点在肝癌组的突变率显著高于慢乙肝组,差异有统计学意义(P＜0.05),而G1899A在两组之间差别无统计学意义.(4)另在前C区发现有插入及缺失突变,均相对点突变发生率较低.结论 HBV前C基因区G1896A位点突变与HCC发生密切相关.
目的 探討乙型肝炎病毒(HBV)前C區基因變異與肝細胞癌(HCC)髮生的關繫.方法 收集在山西醫科大學第一醫院感染病科門診及住院的患者血清及病例資料共99例,其中慢乙肝55例,肝癌44例.採用半巢式聚閤酶連反應及直接測序法檢測前C區基因變異的情況.分彆檢測兩組患者的乙肝病毒標誌物、HBV DNA,採用病例對照研究方法對患者的年齡、性彆、HBeAg、HBVDNA基線水平和HBV前C區基因變異位點進行分析.結果 (1)肝癌患者的HBeAg陰性率均高于慢乙肝組,差異有統計學意義(P＜0.05).(2)肝癌組患者HBV DNA載量低于慢乙肝組,差異有統計學意義(P＜0.05).(3)共髮現前C區有G1896A、G1899A兩箇熱點突變位點,其中G1896A位點在肝癌組的突變率顯著高于慢乙肝組,差異有統計學意義(P＜0.05),而G1899A在兩組之間差彆無統計學意義.(4)另在前C區髮現有插入及缺失突變,均相對點突變髮生率較低.結論 HBV前C基因區G1896A位點突變與HCC髮生密切相關.
목적 탐토을형간염병독(HBV)전C구기인변이여간세포암(HCC)발생적관계.방법 수집재산서의과대학제일의원감염병과문진급주원적환자혈청급병례자료공99례,기중만을간55례,간암44례.채용반소식취합매련반응급직접측서법검측전C구기인변이적정황.분별검측량조환자적을간병독표지물、HBV DNA,채용병례대조연구방법대환자적년령、성별、HBeAg、HBVDNA기선수평화HBV전C구기인변이위점진행분석.결과 (1)간암환자적HBeAg음성솔균고우만을간조,차이유통계학의의(P＜0.05).(2)간암조환자HBV DNA재량저우만을간조,차이유통계학의의(P＜0.05).(3)공발현전C구유G1896A、G1899A량개열점돌변위점,기중G1896A위점재간암조적돌변솔현저고우만을간조,차이유통계학의의(P＜0.05),이G1899A재량조지간차별무통계학의의.(4)령재전C구발현유삽입급결실돌변,균상대점돌변발생솔교저.결론 HBV전C기인구G1896A위점돌변여HCC발생밀절상관.
Objective The aim of the study was to investigate the mutation of precore (PreC) region of hepatitis B virus (HBV) gene and its association with hepatocellular carcinoma.Methods A total of 99 HBV patients were divided into two groups:chronic hepatitis B (CHB,55) and hepatocellular carcinoma (HCC,44).The precore gene fragment was amplified by nested PCR and analyzed by direct DNA sequencing.To analyze the relationships between HCC occurrence and age,sex,baseline HBV DNA,HBeAg status,precore region gene mutation was analyzed in two groups by using the method of case-control.Results There was significant difference between the patients with hepatocellular carcinoma and chronic HBV infection in the baseline levels of HBV DNA.In HBV precore promote region,the common point mutations were G1896A and G1899A.Compared with patients with chronic hepatitis B,point mutation G1896A,patients with liver cancer had a higher incidence of point mutation G1896A.The negative rate of HBeAg in patients with mutation with G1896A was much higher in CHB group than HCC group,the difference was significant (P ＜ 0.05).There was no significant difference between hepatocellular carcinoma and chronic HBV infection in the mutation rate of G1899A (P ＞ 0.05).The rate of insertion or deletion mutations in HBV DNA Precore gene were lower than point mutation.Conclusion HBV PC 1896 mutation was correlated with the occurrence of hepatocellular carcinoma.
