中华医学杂志
中華醫學雜誌
중화의학잡지
National Medical Journal of China
2015年
17期
1341-1344
,共4页
朱鸣镝%沈妍%吴小波%张志军
硃鳴鏑%瀋妍%吳小波%張誌軍
주명적%침연%오소파%장지군
骨肿瘤%疼痛%脊髓%血红素加氧酶1
骨腫瘤%疼痛%脊髓%血紅素加氧酶1
골종류%동통%척수%혈홍소가양매1
Bone neoplasms%Pain%Spinal cord%Heme oxygenase 1
目的 探讨脊髓中过表达血红素加氧酶1(HO-1)对小鼠骨癌性疼痛的缓解作用.方法 小鼠右股骨内注射前列腺癌细胞RM-1构建骨癌性疼痛模型;HE染色检测股骨骨质破坏和骨肿瘤形成情况;疼痛行为学检测骨癌痛小鼠的机械性触诱发痛和热痛觉过敏行为;免疫印迹检测腹腔注射HO-1诱导剂(CoPP)和HO-1抑制剂(SnPP)后脊髓中HO-1蛋白含量的变化.结果 右股骨内注射前列腺癌细胞RM-1后,骨质中出现癌细胞浸润,并且有骨质破坏.前列腺癌细胞RM-1右股骨注射后7d,小鼠右后爪的缩爪阈值和缩爪潜伏期明显下降,并维持21 d以上[缩爪阈值实验组为(0.12±0.02)g比对照组为(1.2±0.37)g,(P＜0.001);缩爪潜伏期实验组(7.4±0.7)s比对照组(11.6±0.2)s,P＜0.001];连续5d腹腔注射HO-1诱导剂CoPP能增加骨癌性疼痛小鼠缩爪阈值和缩爪潜伏期,而HO-1抑制剂SnPP对骨癌性疼痛的疼痛行为无影响.免疫印迹结果显示腹腔注射CoPP后能使骨癌痛小鼠脊髓中HO-1表达上调增加了4.16倍,腹腔注射SnPP不影响骨癌性疼痛小鼠脊髓中HO-1的表达.结论 前列腺癌细胞RM-1股骨内注射能诱导骨癌形成,并诱发骨癌性疼痛行为,上调小鼠脊髓中HO-1表达能缓解骨癌性疼痛.
目的 探討脊髓中過錶達血紅素加氧酶1(HO-1)對小鼠骨癌性疼痛的緩解作用.方法 小鼠右股骨內註射前列腺癌細胞RM-1構建骨癌性疼痛模型;HE染色檢測股骨骨質破壞和骨腫瘤形成情況;疼痛行為學檢測骨癌痛小鼠的機械性觸誘髮痛和熱痛覺過敏行為;免疫印跡檢測腹腔註射HO-1誘導劑(CoPP)和HO-1抑製劑(SnPP)後脊髓中HO-1蛋白含量的變化.結果 右股骨內註射前列腺癌細胞RM-1後,骨質中齣現癌細胞浸潤,併且有骨質破壞.前列腺癌細胞RM-1右股骨註射後7d,小鼠右後爪的縮爪閾值和縮爪潛伏期明顯下降,併維持21 d以上[縮爪閾值實驗組為(0.12±0.02)g比對照組為(1.2±0.37)g,(P＜0.001);縮爪潛伏期實驗組(7.4±0.7)s比對照組(11.6±0.2)s,P＜0.001];連續5d腹腔註射HO-1誘導劑CoPP能增加骨癌性疼痛小鼠縮爪閾值和縮爪潛伏期,而HO-1抑製劑SnPP對骨癌性疼痛的疼痛行為無影響.免疫印跡結果顯示腹腔註射CoPP後能使骨癌痛小鼠脊髓中HO-1錶達上調增加瞭4.16倍,腹腔註射SnPP不影響骨癌性疼痛小鼠脊髓中HO-1的錶達.結論 前列腺癌細胞RM-1股骨內註射能誘導骨癌形成,併誘髮骨癌性疼痛行為,上調小鼠脊髓中HO-1錶達能緩解骨癌性疼痛.
목적 탐토척수중과표체혈홍소가양매1(HO-1)대소서골암성동통적완해작용.방법 소서우고골내주사전렬선암세포RM-1구건골암성동통모형;HE염색검측고골골질파배화골종류형성정황;동통행위학검측골암통소서적궤계성촉유발통화열통각과민행위;면역인적검측복강주사HO-1유도제(CoPP)화HO-1억제제(SnPP)후척수중HO-1단백함량적변화.결과 우고골내주사전렬선암세포RM-1후,골질중출현암세포침윤,병차유골질파배.전렬선암세포RM-1우고골주사후7d,소서우후조적축조역치화축조잠복기명현하강,병유지21 d이상[축조역치실험조위(0.12±0.02)g비대조조위(1.2±0.37)g,(P＜0.001);축조잠복기실험조(7.4±0.7)s비대조조(11.6±0.2)s,P＜0.001];련속5d복강주사HO-1유도제CoPP능증가골암성동통소서축조역치화축조잠복기,이HO-1억제제SnPP대골암성동통적동통행위무영향.면역인적결과현시복강주사CoPP후능사골암통소서척수중HO-1표체상조증가료4.16배,복강주사SnPP불영향골암성동통소서척수중HO-1적표체.결론 전렬선암세포RM-1고골내주사능유도골암형성,병유발골암성동통행위,상조소서척수중HO-1표체능완해골암성동통.
Objective To explore the anti-nociceptive effects of heme oxygenase 1 (HO-1) on bone cancer pain in mice.Methods The model of bone cancer pain was established by an inoculation of prostate tumor cell line RM-1 cells into intramedullary space of murine femur.Bone destruction and tumor formation in femur were checked by hematoxylin and eosin staining.The mechanical allodynia and heat hyperalgesia were examined by behavioral testing.After intraperitoneal injections of HO-1 inducer (CoPP) and HO-1 inhibitor (SnPP),the protein content of HO-1 in spinal cord was examined by Western blot.Results The invasion of tumor cells into bone and bone destruction were induced by an injection of RM-1 into right femur.The paw withdrawal threshold (PWL) and paw withdrawal latency (PWL) decreased significantly at 7 days after inoculation and maintained for over 21 days.HO-1 inducer CoPP increased PWT and PWL dramatically in mice with bone cancer pain after consecutive intraperitoneal dosing of CoPP for 5 days.However,SnPP unaltered pain behaviors.Western blot showed the expression of HO-1 in spinal cord was upregulated after an intraperitoneal injection of CoPP and unchanged after an intraperitoneal injection of SnPP.Conclusion Intramedullary inoculation of prostate tumor cell line RM-1 cells produces bone cancer and induces bone cancer pain.An up-regulation of HO-1 in spinal cord may alleviate bone cancer pain.
