中华口腔医学杂志
中華口腔醫學雜誌
중화구강의학잡지
Chinese Journal of Stomatology
2015年
5期
307-312
,共6页
氟中毒,牙%牙釉质%显微镜检查,原子力%显微镜检查,电子,扫描
氟中毒,牙%牙釉質%顯微鏡檢查,原子力%顯微鏡檢查,電子,掃描
불중독,아%아유질%현미경검사,원자력%현미경검사,전자,소묘
Fluorosis,dental%Dental,enamel%Microscopy,atomic force%Microscopy,electron,scanning
目的 观察大鼠氟斑牙模型下颌切牙釉质的超微结构,探讨过量氟对牙体微观结构的影响.方法 30只5周龄SD大鼠雌雄各半,均分为3组,对照组用不含氟的去离子水喂养,低氟组用含22.5 mg/L F-(50 mg/L NaF)、高氟组用含45 mg/L F-(100 mg/L NaF)的去离子水喂养;饲养8周后,取所有大鼠下颌切牙,制备用于扫描电镜和原子力显微镜(atomic force microscopy,AFM)观察的牙体组织切片,分析比较不同氟浓度对大鼠釉质微观结构的影响.结果 扫描电镜结果显示,对照组样本釉质中釉柱结构完整,晶体排列紧密,层次清晰;高氟组样本釉柱结构瓦解,晶体松散、弯曲折断;低氟组样本釉柱结构形态的变化介于对照组与高氟组之间.AFM结果显示,对照组、低氟组及高氟组样本釉质中层的平均粗糙度分别为(550.6±32.0)、(415.0±24.2)及(194.3±11.3) nm,高氟组与对照组相比差异有统计学意义(P＜0.05).结论 在高氟环境下形成的大鼠牙釉质出现明显的结构坍塌,釉柱间无间隙;高氟可造成大鼠发育中牙釉质的釉柱晶体正常结构丧失.
目的 觀察大鼠氟斑牙模型下頜切牙釉質的超微結構,探討過量氟對牙體微觀結構的影響.方法 30隻5週齡SD大鼠雌雄各半,均分為3組,對照組用不含氟的去離子水餵養,低氟組用含22.5 mg/L F-(50 mg/L NaF)、高氟組用含45 mg/L F-(100 mg/L NaF)的去離子水餵養;飼養8週後,取所有大鼠下頜切牙,製備用于掃描電鏡和原子力顯微鏡(atomic force microscopy,AFM)觀察的牙體組織切片,分析比較不同氟濃度對大鼠釉質微觀結構的影響.結果 掃描電鏡結果顯示,對照組樣本釉質中釉柱結構完整,晶體排列緊密,層次清晰;高氟組樣本釉柱結構瓦解,晶體鬆散、彎麯摺斷;低氟組樣本釉柱結構形態的變化介于對照組與高氟組之間.AFM結果顯示,對照組、低氟組及高氟組樣本釉質中層的平均粗糙度分彆為(550.6±32.0)、(415.0±24.2)及(194.3±11.3) nm,高氟組與對照組相比差異有統計學意義(P＜0.05).結論 在高氟環境下形成的大鼠牙釉質齣現明顯的結構坍塌,釉柱間無間隙;高氟可造成大鼠髮育中牙釉質的釉柱晶體正常結構喪失.
목적 관찰대서불반아모형하합절아유질적초미결구,탐토과량불대아체미관결구적영향.방법 30지5주령SD대서자웅각반,균분위3조,대조조용불함불적거리자수위양,저불조용함22.5 mg/L F-(50 mg/L NaF)、고불조용함45 mg/L F-(100 mg/L NaF)적거리자수위양;사양8주후,취소유대서하합절아,제비용우소묘전경화원자력현미경(atomic force microscopy,AFM)관찰적아체조직절편,분석비교불동불농도대대서유질미관결구적영향.결과 소묘전경결과현시,대조조양본유질중유주결구완정,정체배렬긴밀,층차청석;고불조양본유주결구와해,정체송산、만곡절단;저불조양본유주결구형태적변화개우대조조여고불조지간.AFM결과현시,대조조、저불조급고불조양본유질중층적평균조조도분별위(550.6±32.0)、(415.0±24.2)급(194.3±11.3) nm,고불조여대조조상비차이유통계학의의(P＜0.05).결론 재고불배경하형성적대서아유질출현명현적결구담탑,유주간무간극;고불가조성대서발육중아유질적유주정체정상결구상실.
Objective To investigate the ultrastructure of enamel in the mandibular incisor tooth in a rat model of dental fluorosis.Methods Thirty 5-week-old male and female rats of SD strain were divided into three groups of ten.The animals in the control group were maintained for 8 weeks on pure deionized water,in the low-fluoride group deionized water with 22.5 mg/L of fluorine(50 mg/L NaF) was used,and in the high-fluoride group deionized water with 45 mg/L of fluorine(100 mg/L NaF) was used.All specimens examined were obtained from the mandibular incisors.Mid-sagittal ground sections were prepared from the direction of incisal point of each incisor for scanning electron microscopy(SEM) and atomic force microscopy (AFM).Results According to the SEM results,the prisms in the control group had a complete and clear column structure with closely packed enamel rods or enamel prisms.The prisms in the high-fluoride experimental group were collapsed and showed curved and fractured crystallites.The level of prism structural changes in the low-fluoride experimental group was in between that of the control group and the high-fluoride group.AFM for the middle layer enamel showed an average roughness(Ra) of (550.6±32.0) nm in the control group,(415.0±24.2) nm in the low-fluoride group,and (194.3± 11.3) nm for the high-fluoride group.There was significant difference between the high-fluoride group and the control group(P＜0.05).Conclusions Formation of rat enamel in the high fluoride environment presented obvious structural collapse and no clearance between prisms.Such changes appear to indicate a loss of normal prism structure in rat enamels from exposure to high levels of fluorine during the maturation period.
