CAJ | 학술논문

对6周耐力训练及一次性定量负荷运动后大鼠心肌组织用分光光度法和RT-PCR检测法进行GSH-Px活性和mRNA的表达，结果显示：6周耐力训练后进行一次性定量负荷运动大鼠心肌GSH-Px mRNA的表达升高，激活了心肌GSH-Px mRNA表达，进而引起总抗氧化能力的提高，增强了机体清除自由基的能力，有效的起到氧化预适应的作用；GSH-Px活性显著升高（p＜0.05），6周耐力训练组3h的定量负荷运动后即刻机体心肌GSH-Px mRNA表达被激活，提高了GSH-Px的活性，总抗氧化能力也得到明显的提高，清除自由基的能力增强，减少了自由基对机体的损伤，提高了机体抗运动疲劳的能力。
대6주내력훈련급일차성정량부하운동후대서심기조직용분광광도법화RT-PCR검측법진행GSH-Px활성화mRNA적표체，결과현시：6주내력훈련후진행일차성정량부하운동대서심기GSH-Px mRNA적표체승고，격활료심기GSH-Px mRNA표체，진이인기총항양화능력적제고，증강료궤체청제자유기적능력，유효적기도양화예괄응적작용；GSH-Px활성현저승고（p＜0.05），6주내력훈련조3h적정량부하운동후즉각궤체심기GSH-Px mRNA표체피격활，제고료GSH-Px적활성，총항양화능력야득도명현적제고，청제자유기적능력증강，감소료자유기대궤체적손상，제고료궤체항운동피로적능력。
The research adopted spectrophotometric and RT-PCR technology to research GSH-Px activity and mRNA expression in rats’cardiac muscles that six weeks of endurance training and disposable after quantitative load exercise. The results showed that six weeks after endurance training for one-time quantitative load exercise rats myocardial GSH-Px mRNA expression increased,GSH-Px activity significantly increased (p<0.05). Conclusion:(1)six week endurance training activation of myocardial GSH-Px mRNA expression,thereby causing the total antioxidant capacity,enhance the body’s ability to eliminate free radicals,effectively play the role of oxidative preconditioning effect.(2)After 6 weeks of endurance training by 3h of quantitative load movement immediately,the activity of GSH-Px was raising,and the total antioxidant capacity has been significantly improved . therefore the free radicals was removed and decreased the damage to the body ,it improve that the body’s ability of Anti Sports Fatigue and extend exercise time.