CAJ | 학술논문

文章主要探讨弱恒定电场对铁氮共掺二氧化钛纳米颗粒光催化灭活HL60细胞实验的影响以及其机理。利用细胞计数法、CCK-8法检测、倒置显微镜、活性氧检测和荧光光谱等手段进行了分析研究，结果表明，在无电场作用下，此纳米颗粒光催化灭活HL60细胞的效率达到75．07％，然而在弱恒定电场的作用下，其效率将提高，在场强为600 mV／mm的电场作用下，其效率可达到80．13％，同时，对细胞膜的损伤程度也更大，产生的活性氧类物质也更多；荧光光谱分析推测，电场可能通过分离光生空穴电子对和提供能量给价带电子跃迁来使更多空穴和电子被利用，进而提高此纳米颗粒的光催化活性。
문장주요탐토약항정전장대철담공참이양화태납미과립광최화멸활HL60세포실험적영향이급기궤리。이용세포계수법、CCK-8법검측、도치현미경、활성양검측화형광광보등수단진행료분석연구，결과표명，재무전장작용하，차납미과립광최화멸활HL60세포적효솔체도75．07％，연이재약항정전장적작용하，기효솔장제고，재장강위600 mV／mm적전장작용하，기효솔가체도80．13％，동시，대세포막적손상정도야경대，산생적활성양류물질야경다；형광광보분석추측，전장가능통과분리광생공혈전자대화제공능량급개대전자약천래사경다공혈화전자피이용，진이제고차납미과립적광최화활성。
The influence of the low constant electric field on the photocatalytic inactivation of titanium dioxide nanopar-ticles co-doped with Fe &N (Fe-N-TiO2 nanoparticles)on HL60 cells and its mechanism were studied in this paper. CCK-8,the inverted microscope,the fluorescence spectra of reactive oxygen species and the fluorescence spectra of nan-oparticles were used to study the influence of the low constant electric field on the photocatalytic inactivation of Fe-N-TiO2 nanoparticles on HL60 cells and to explore preliminarily the mechanism.The results showed that the photocatalytic inactivation efficiency of this nanoparticles on HL60 cells was 75 .07% in no electric field,while the efficiency in the low constant electric field would be developed.In the constant electric field of 600 mV/mm,the efficiency could reach to 80.13%,meanwhile,the injury degree was higher and the fluorescence intensity of the reactive oxygen species was stronger.The fluorescence spectra of the nanoparticles indicated that the electric field may improve the photocatalytic ac-tivity of the nanoparticles by separating the photogenerated hole-electron pairs and providing energy to electric transitions of the valence band to make more holes and electrons be used.