CAJ | 학술논문

目的：探讨白细胞介素4（ IL-4）－590位点和IL-13－1112位点的单核苷酸多态性（ SNP）与支气管哮喘的关系及其对表型血浆总IgE（ TIgE）水平的影响。方法应用聚合酶链反应-限制性片段长度多态性分析方法（ PCR-RFLP），检测250例哮喘组儿童和200例对照组儿童血浆两基因位点的多态性；用酶联免疫吸附法（ELISA）检测两组儿童TIgE的水平。结果（1）两个位点的基因型频率在哮喘组和对照组间的分布差异有统计学意义（P均＜0．01）。（2）哮喘组和对照组中IL-4－590C／T位点C等位基因频率分别为23．00％和35．25％，T等位基因频率分别为77．00％和64．75％。 IL-13－1112C／T位点C等位基因频率分别为59．20％和70．50％，T等位基因频率分别为40．80％和29．50％。两组之间等位基因频率分布差异有统计学意义（P均＜0．01）。（3）两个位点的变异等位基因携带者患哮喘危险性高于非携带者（P均＜0．01），OR分别为1．82（95％CI 1．36～2．44）和1．647（95％CI 1．246～2．178）。（4）两位点各基因型血浆TIgE水平哮喘组均高于对照组（P＜0．05）。（5）IL-13－1112C／T位点变异等位基因T携带者与非携带者TIgE水平比较，P＞0．05；IL-4－590C／T位点哮喘组变异等位基因T携带者TIgE水平与非携带者比较，P＜0．05。结论两位点T等位基因与哮喘的发病有相关性。 IL-4－590C／T位点变异等位基因T使血浆TIgE水平升高。 IL-13－1112C／T位点变异等位基因T与血浆TIgE水平无明显相关性。
목적：탐토백세포개소4（ IL-4）－590위점화IL-13－1112위점적단핵감산다태성（ SNP）여지기관효천적관계급기대표형혈장총IgE（ TIgE）수평적영향。방법응용취합매련반응-한제성편단장도다태성분석방법（ PCR-RFLP），검측250례효천조인동화200례대조조인동혈장량기인위점적다태성；용매련면역흡부법（ELISA）검측량조인동TIgE적수평。결과（1）량개위점적기인형빈솔재효천조화대조조간적분포차이유통계학의의（P균＜0．01）。（2）효천조화대조조중IL-4－590C／T위점C등위기인빈솔분별위23．00％화35．25％，T등위기인빈솔분별위77．00％화64．75％。 IL-13－1112C／T위점C등위기인빈솔분별위59．20％화70．50％，T등위기인빈솔분별위40．80％화29．50％。량조지간등위기인빈솔분포차이유통계학의의（P균＜0．01）。（3）량개위점적변이등위기인휴대자환효천위험성고우비휴대자（P균＜0．01），OR분별위1．82（95％CI 1．36～2．44）화1．647（95％CI 1．246～2．178）。（4）량위점각기인형혈장TIgE수평효천조균고우대조조（P＜0．05）。（5）IL-13－1112C／T위점변이등위기인T휴대자여비휴대자TIgE수평비교，P＞0．05；IL-4－590C／T위점효천조변이등위기인T휴대자TIgE수평여비휴대자비교，P＜0．05。결론량위점T등위기인여효천적발병유상관성。 IL-4－590C／T위점변이등위기인T사혈장TIgE수평승고。 IL-13－1112C／T위점변이등위기인T여혈장TIgE수평무명현상관성。
Objective To investigate the correlation between the single nucleotide polymorphisms (SNPs) of IL-4 gene -590C/T as well as IL-13 gene -1112C/T and the incidence of asthma in children.To address whether the SNPs of two loci have any impact on total serum IgE( TIgE) levels.Methods Polymerase chain reaction-restriction fragment length polymorphism technique( PCR-RFLP) was used to determine the two locus polymorphisms of 250 patients with asthma and 200 healthy subjects in control group.The level of TIgE was tested by enzyme-linked immunosorbent assay(ELISA) in two groups.Results (1)The genotype distribu-tion in each locus was different in two groups(P<0.01).(2)In patient and control groups, the C allele fre-quency at IL-4-590 locus was 23.00%and 35.25%,the T allele frequency at the same locus was 77.00%and 64.75%, respectively.Besides, the C allele frequency at IL-13 -1112 locus was 59.20%and 70.50%,the T allele frequency at the same locus was 40.80%and 29.50%, respectively.The allele frequencies at each locus had the significant difference in asthma and control group(P<0.01).(3)The subjects with variant allele have higher risks for asthma than those without variant allele ( OR=1.82, 95%CI=1.36-2.44, and OR=1.647, 95%CI=1.246-2.178).(4)The TIgE level of asthma group was higher than that of control group in those with the same genotype (P<0.05).(5)No significant difference with the TIgE levels was observed in those with or without variant T allele at IL-13 -1112 locus(P>0.05), which was in contrast with that at IL-4 -590 locus in asthma group(P<0.05).Conclusion The SNPs of two loci were associated with childhood asthma.Variant al-lele T at 590C/T locus in IL-4 gene correlated with higher serum IgE levels.There was no significant correlation between the serum IgE levels and variant T allele at IL-13 -1112C/T locus.