中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2015年
4期
258-264
,共7页
俞俊岭%吴建军%胡中旺%类延花%李国兰%张文昌%赵俊%张俊玲%甘霖%余莉%陈敬贤%王明丽
俞俊嶺%吳建軍%鬍中旺%類延花%李國蘭%張文昌%趙俊%張俊玲%甘霖%餘莉%陳敬賢%王明麗
유준령%오건군%호중왕%류연화%리국란%장문창%조준%장준령%감림%여리%진경현%왕명려
人类免疫缺陷病毒%人巨细胞病毒%限制性片段长度多态性分析
人類免疫缺陷病毒%人巨細胞病毒%限製性片段長度多態性分析
인류면역결함병독%인거세포병독%한제성편단장도다태성분석
HIV%HCMV%Restricted fragment length polymorphism analysis ( RFLP)
目的:了解HIV感染者中人巨细胞病毒( human cytomegalovirus, HCMV)糖蛋白N ( glycoprotein N, gN)的型别分布;探讨HCMV-HIV共感染对患者疾病进程的影响及不同gN基因型感染与获得性免疫缺陷综合征( acquired immuno-deficiency syndrome,AIDS)发病和相关死亡的关系。方法对359例HIV感染者进行HCMV pp65抗原血症检测,利用HCMV gN(UL73)基因巢式PCR扩增HCMV活动性感染患者全血DNA,限制性核酸内切酶MboⅠ、ScaⅠ和SalⅠ对巢式PCR产物进行限制性片段长度多态性分析( restricted fragment length polymorphism analysis, RFLP),队列随访研究不同型别HCMV感染与AIDS患者发病和死亡的关系。结果 HCMV pp65抗原血症检测发现,359例HIV感染者中HCMV活动性感染患者28例,对其中20例HCMV活动性感染患者的全血DNA样本进行PCR-RFLP分析,结果表明,HIV感染患者中HCMV gN基因型分布如下:gN-3a,4/20(20%);gN-1,4/20(20%);gN-4b,1/20(5%);gN-4d,1/20(5%);混合感染,10/20(50%);在随访期内,HCMV UL73基因阳性的HIV感染者更易转变为AIDS患者(RR=9.78),且gN-1和gN-4型感染者发病和相关死亡风险是其他基因型的4.6倍(P<0.05)。结论 HCMV活动性感染可能会加速HIV感染者疾病进程,其中以gN-1和gN-4型HCMV活动性感染相对危险度最高。
目的:瞭解HIV感染者中人巨細胞病毒( human cytomegalovirus, HCMV)糖蛋白N ( glycoprotein N, gN)的型彆分佈;探討HCMV-HIV共感染對患者疾病進程的影響及不同gN基因型感染與穫得性免疫缺陷綜閤徵( acquired immuno-deficiency syndrome,AIDS)髮病和相關死亡的關繫。方法對359例HIV感染者進行HCMV pp65抗原血癥檢測,利用HCMV gN(UL73)基因巢式PCR擴增HCMV活動性感染患者全血DNA,限製性覈痠內切酶MboⅠ、ScaⅠ和SalⅠ對巢式PCR產物進行限製性片段長度多態性分析( restricted fragment length polymorphism analysis, RFLP),隊列隨訪研究不同型彆HCMV感染與AIDS患者髮病和死亡的關繫。結果 HCMV pp65抗原血癥檢測髮現,359例HIV感染者中HCMV活動性感染患者28例,對其中20例HCMV活動性感染患者的全血DNA樣本進行PCR-RFLP分析,結果錶明,HIV感染患者中HCMV gN基因型分佈如下:gN-3a,4/20(20%);gN-1,4/20(20%);gN-4b,1/20(5%);gN-4d,1/20(5%);混閤感染,10/20(50%);在隨訪期內,HCMV UL73基因暘性的HIV感染者更易轉變為AIDS患者(RR=9.78),且gN-1和gN-4型感染者髮病和相關死亡風險是其他基因型的4.6倍(P<0.05)。結論 HCMV活動性感染可能會加速HIV感染者疾病進程,其中以gN-1和gN-4型HCMV活動性感染相對危險度最高。
목적:료해HIV감염자중인거세포병독( human cytomegalovirus, HCMV)당단백N ( glycoprotein N, gN)적형별분포;탐토HCMV-HIV공감염대환자질병진정적영향급불동gN기인형감염여획득성면역결함종합정( acquired immuno-deficiency syndrome,AIDS)발병화상관사망적관계。방법대359례HIV감염자진행HCMV pp65항원혈증검측,이용HCMV gN(UL73)기인소식PCR확증HCMV활동성감염환자전혈DNA,한제성핵산내절매MboⅠ、ScaⅠ화SalⅠ대소식PCR산물진행한제성편단장도다태성분석( restricted fragment length polymorphism analysis, RFLP),대렬수방연구불동형별HCMV감염여AIDS환자발병화사망적관계。결과 HCMV pp65항원혈증검측발현,359례HIV감염자중HCMV활동성감염환자28례,대기중20례HCMV활동성감염환자적전혈DNA양본진행PCR-RFLP분석,결과표명,HIV감염환자중HCMV gN기인형분포여하:gN-3a,4/20(20%);gN-1,4/20(20%);gN-4b,1/20(5%);gN-4d,1/20(5%);혼합감염,10/20(50%);재수방기내,HCMV UL73기인양성적HIV감염자경역전변위AIDS환자(RR=9.78),차gN-1화gN-4형감염자발병화상관사망풍험시기타기인형적4.6배(P<0.05)。결론 HCMV활동성감염가능회가속HIV감염자질병진정,기중이gN-1화gN-4형HCMV활동성감염상대위험도최고。
Objective To analyze the distribution of various genotypes of human cytomegalovirus glycoprotein N ( HCMV gN) in patients with HIV infection; to investigate the effects of HCMV-HIV co-in-fection on disease progression and the relationships between HCMV gN genotypes and disease progression. Methods Patients with active HCMV infection were screened out from 359 patients with HIV infection by using the pp65 antigenemia assay.The genes encoding HCMV gN ( UL73 ) were amplified by nested PCR ( nPCR) .The amplicons were digested by restriction enzymes including MboⅠ, ScaⅠ and SalⅠ.Then, the restricted fragment length polymorphisms were further analyzed on 4%agarose gel.The relationships be-tween HCMV genotypes and the morbidity and mortality of acquired immune deficiency syndrome ( AIDS ) were investigated via a prospective study.Results Among the 359 patients with HIV infection, 28 subjects were positive for the HCMV pp65 antigenemia assay.The HCMV gN genotypes in 20 patients with active HCMV infection were distributed as: gN-3a (4/20, 20%), gN-1 (4/20, 20%), gN-4d (1/20, 5%), gN-4b (1/20, 5%) and mixed infection (10/20, 50%).Patients with HCMV-HIV co-infection were more likely to develop AIDS during the follow-up period (RR=9.78).Patients harboring HCMV gN-1 and gN-4 genotypes would seem likely to have 4.6 times of chance leading to AIDS-associated death than those harbo-ring other HCMV gN genotypes.Conclusion HCMV infection ( especially gN-1 and gN-4 genotypes) might accelerate the progression of HIV infection.