中华微生物学和免疫学杂志
中華微生物學和免疫學雜誌
중화미생물학화면역학잡지
CHINESE JOURNAL OF MICROBIOLOGY AND IMMUNOLOGY
2015年
4期
253-257
,共5页
许光银%裴耀文%张世英%王志玉
許光銀%裴耀文%張世英%王誌玉
허광은%배요문%장세영%왕지옥
柯萨奇病毒A组16型%基因特征%手足口病%B2 b亚型
柯薩奇病毒A組16型%基因特徵%手足口病%B2 b亞型
가살기병독A조16형%기인특정%수족구병%B2 b아형
Coxsackievirus A16%Genetic characteristics%Hand,foot and mouth disease%B2b sub-genotype
目的：分析2013年聊城市手足口病（ hand foot and mouth disease， HFMD）病原构成及柯萨奇病毒A组16型（coxsackievirus A16， CVA16）VP1区基因特征。方法采集聊城市2013年1—12月监测点医院手足口病患儿的粪便和咽拭子标本，采用RT-PCR方法对HFMD疑似病例标本进行病原鉴定，选取CVA16核酸阳性标本，使用RD细胞和Vero细胞进行病毒分离。对9株CVA16病毒阳性的分离物进行VP1区基因扩增及核苷酸序列测定，与其他各基因型及亚型的代表株序列构建亲缘进化树，并进行核苷酸和氨基酸同源性分析。结果2013年聊城市共检测手足口病病例标本961份，阳性者747份，阳性率77．73％。其中人肠道病毒71型（ enterovirus 71， EV71）74份，占阳性标本的9．91％，CVA16130份，占17．40％；其他肠道病毒543份，占72．69％。应用 BioEdit 和 MEGA 4软件分析发现，9株CVA16彼此间VP1核苷酸和氨基酸水平同源性较高，分别在97．7％～100％和99．3％～100％之间。亲缘进化树分析显示山东聊城CVA16分离株与B2b基因亚型的代表株处于同一分支，属于B2b基因亚型。结论2013年引起聊城市手足口病的主要病原体以其他肠道病毒为主，兼有CVA16和EV71参与，而CVA16属于B2b亚型。手足口病的病原谱正发生改变，应加强EV71、CVA16及其他肠道病毒的分子流行病学监测，了解其基因特征，对预防和控制手足口病具有重要意义。
目的：分析2013年聊城市手足口病（ hand foot and mouth disease， HFMD）病原構成及柯薩奇病毒A組16型（coxsackievirus A16， CVA16）VP1區基因特徵。方法採集聊城市2013年1—12月鑑測點醫院手足口病患兒的糞便和嚥拭子標本，採用RT-PCR方法對HFMD疑似病例標本進行病原鑒定，選取CVA16覈痠暘性標本，使用RD細胞和Vero細胞進行病毒分離。對9株CVA16病毒暘性的分離物進行VP1區基因擴增及覈苷痠序列測定，與其他各基因型及亞型的代錶株序列構建親緣進化樹，併進行覈苷痠和氨基痠同源性分析。結果2013年聊城市共檢測手足口病病例標本961份，暘性者747份，暘性率77．73％。其中人腸道病毒71型（ enterovirus 71， EV71）74份，佔暘性標本的9．91％，CVA16130份，佔17．40％；其他腸道病毒543份，佔72．69％。應用 BioEdit 和 MEGA 4軟件分析髮現，9株CVA16彼此間VP1覈苷痠和氨基痠水平同源性較高，分彆在97．7％～100％和99．3％～100％之間。親緣進化樹分析顯示山東聊城CVA16分離株與B2b基因亞型的代錶株處于同一分支，屬于B2b基因亞型。結論2013年引起聊城市手足口病的主要病原體以其他腸道病毒為主，兼有CVA16和EV71參與，而CVA16屬于B2b亞型。手足口病的病原譜正髮生改變，應加彊EV71、CVA16及其他腸道病毒的分子流行病學鑑測，瞭解其基因特徵，對預防和控製手足口病具有重要意義。
목적：분석2013년료성시수족구병（ hand foot and mouth disease， HFMD）병원구성급가살기병독A조16형（coxsackievirus A16， CVA16）VP1구기인특정。방법채집료성시2013년1—12월감측점의원수족구병환인적분편화인식자표본，채용RT-PCR방법대HFMD의사병례표본진행병원감정，선취CVA16핵산양성표본，사용RD세포화Vero세포진행병독분리。대9주CVA16병독양성적분리물진행VP1구기인확증급핵감산서렬측정，여기타각기인형급아형적대표주서렬구건친연진화수，병진행핵감산화안기산동원성분석。결과2013년료성시공검측수족구병병례표본961빈，양성자747빈，양성솔77．73％。기중인장도병독71형（ enterovirus 71， EV71）74빈，점양성표본적9．91％，CVA16130빈，점17．40％；기타장도병독543빈，점72．69％。응용 BioEdit 화 MEGA 4연건분석발현，9주CVA16피차간VP1핵감산화안기산수평동원성교고，분별재97．7％～100％화99．3％～100％지간。친연진화수분석현시산동료성CVA16분리주여B2b기인아형적대표주처우동일분지，속우B2b기인아형。결론2013년인기료성시수족구병적주요병원체이기타장도병독위주，겸유CVA16화EV71삼여，이CVA16속우B2b아형。수족구병적병원보정발생개변，응가강EV71、CVA16급기타장도병독적분자류행병학감측，료해기기인특정，대예방화공제수족구병구유중요의의。
Objective To study the etiological agent of hand, foot and mouth disease ( HFMD) and the genetic characteristics of coxsackievirus A16 ( CVA16 ) strains isolated from clinical specimens of patients with HFMD in Liaocheng city in 2013.Methods Throat swab and stool specimens were collected from patients with HFMD in the disease surveillance hospitals in Liaocheng city from January to December 2013.Samples pos-itive for CVA16 strains were screened out for the isolation of virus strains with rhabdomyosarcoma ( RD) cells and Vero cells.The entire VP1 coding regions of 9 randomly selected CVA16 isolates were amplified and se-quenced.BioEdit and MEGA4 softwares were used for homology analysis.A phylogenetic tree among the 9 CVA16 isolates and 56 CVA16 representative strains of known genotypes and subgenotypes was constructed.Re-sults The results of PCR analysis showed that 747(77.73%) out of 961 specimens were positive for HFMD and among them, 74 samples (9.91%) were positive for EV71 strains, 130(17.40%) were CVA16 strains and 543(72.69%) were other enterovirus strains.The 9 CVA16 strains clustered into the B2b evolution branch of B genotype with the representative strains, sharing 97.7%to 100%homologies in nucleotide sequences and 99.3%to 100%in amino acid sequences.Conclusion Although EV71 and CVA16 strains were identified, other enteric viruses were the predominant pathogens causing HFMD in Liaocheng city in 2013.The CVA16 iso-lates belonged to B2b subgenotype.The pathogen spectrum of HFMD had already changed.It is necessary to strengthen the surveillance for EV71, CVA16 and other enteric viruses and understand their genetic characteriza-tions, which would be of great significance for the prevention and control of HFMD.
