国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
7期
492-496
,共5页
金朝红%李建强%杨婵%柴春维%赵卉
金朝紅%李建彊%楊嬋%柴春維%趙卉
금조홍%리건강%양선%시춘유%조훼
牛磺酸%哮喘大鼠%肌球蛋白轻链激酶%白介素5
牛磺痠%哮喘大鼠%肌毬蛋白輕鏈激酶%白介素5
우광산%효천대서%기구단백경련격매%백개소5
Taurine%Asthmatic rats%Myosin light chain kinase%Interleukin-5
目的 探讨牛磺酸对支气管哮喘(简称哮喘)大鼠气道肌球蛋白轻链激酶(MLCK)和血清IL-5表达的影响.方法 32只清洁级健康SD大鼠随机等分为正常对照组、哮喘模型组、牛磺酸干预组、地塞米松干预组.以卵蛋白和氢氧化铝混悬液诱发大鼠哮喘模型.牛磺酸干预组和地塞米松干预组于雾化前1h分别给予腹腔注射0.5 g/kg牛磺酸和0.5 mg/kg地塞米松.计数BALF中白细胞总数和嗜酸粒细胞数,采用免疫组织化学法检测大鼠气道MLCK的表达,采用双抗体夹心法检测大鼠血清IL-5的含量.结果 ①与正常对照组比较,哮喘模型组MLCK和IL-5水平均较高(t=4.324,P＜0.05;t=3.984,P＜0.05);与哮喘模型组比较,牛磺酸干预组MLCK和IL-5水平均较低(￡=3.987,P＜0.05;t=4.375,P＜0.05);与地塞米松组比较,牛磺酸干预组MLCK和IL-5水平变化不大,差异无统计学意义(t=0.780,P＞0.05;t=0.674,P＞0.05).②牛磺酸干预组和地塞米松干预组的大鼠哮喘症状较哮喘模型组明显减轻,但牛磺酸干预组没有出现体质量减轻,食欲减退等不良反应.结论 牛磺酸可能具有通过降低哮喘大鼠气道MLCK水平参与气道舒张以及减轻气道炎症的作用.
目的 探討牛磺痠對支氣管哮喘(簡稱哮喘)大鼠氣道肌毬蛋白輕鏈激酶(MLCK)和血清IL-5錶達的影響.方法 32隻清潔級健康SD大鼠隨機等分為正常對照組、哮喘模型組、牛磺痠榦預組、地塞米鬆榦預組.以卵蛋白和氫氧化鋁混懸液誘髮大鼠哮喘模型.牛磺痠榦預組和地塞米鬆榦預組于霧化前1h分彆給予腹腔註射0.5 g/kg牛磺痠和0.5 mg/kg地塞米鬆.計數BALF中白細胞總數和嗜痠粒細胞數,採用免疫組織化學法檢測大鼠氣道MLCK的錶達,採用雙抗體夾心法檢測大鼠血清IL-5的含量.結果 ①與正常對照組比較,哮喘模型組MLCK和IL-5水平均較高(t=4.324,P＜0.05;t=3.984,P＜0.05);與哮喘模型組比較,牛磺痠榦預組MLCK和IL-5水平均較低(￡=3.987,P＜0.05;t=4.375,P＜0.05);與地塞米鬆組比較,牛磺痠榦預組MLCK和IL-5水平變化不大,差異無統計學意義(t=0.780,P＞0.05;t=0.674,P＞0.05).②牛磺痠榦預組和地塞米鬆榦預組的大鼠哮喘癥狀較哮喘模型組明顯減輕,但牛磺痠榦預組沒有齣現體質量減輕,食欲減退等不良反應.結論 牛磺痠可能具有通過降低哮喘大鼠氣道MLCK水平參與氣道舒張以及減輕氣道炎癥的作用.
목적 탐토우광산대지기관효천(간칭효천)대서기도기구단백경련격매(MLCK)화혈청IL-5표체적영향.방법 32지청길급건강SD대서수궤등분위정상대조조、효천모형조、우광산간예조、지새미송간예조.이란단백화경양화려혼현액유발대서효천모형.우광산간예조화지새미송간예조우무화전1h분별급여복강주사0.5 g/kg우광산화0.5 mg/kg지새미송.계수BALF중백세포총수화기산립세포수,채용면역조직화학법검측대서기도MLCK적표체,채용쌍항체협심법검측대서혈청IL-5적함량.결과 ①여정상대조조비교,효천모형조MLCK화IL-5수평균교고(t=4.324,P＜0.05;t=3.984,P＜0.05);여효천모형조비교,우광산간예조MLCK화IL-5수평균교저(￡=3.987,P＜0.05;t=4.375,P＜0.05);여지새미송조비교,우광산간예조MLCK화IL-5수평변화불대,차이무통계학의의(t=0.780,P＞0.05;t=0.674,P＞0.05).②우광산간예조화지새미송간예조적대서효천증상교효천모형조명현감경,단우광산간예조몰유출현체질량감경,식욕감퇴등불량반응.결론 우광산가능구유통과강저효천대서기도MLCK수평삼여기도서장이급감경기도염증적작용.
Objective To investigate the effect of taurine (TAU) on expression of myosin light chain kinase (MLCK),interleukin-5 (IL-5) and IgE of bronchial asthma (asthma) rats.Methods Thirtytwo male Sprague-Daw (SD) rats were randomly divided into four groups:normal group (n =8),asthma group (n =8),TAU group (n =8) and DXM group (n =8).The rats were sensitized and challenged with ovalhumin (OVA) to establish chronic asthma model.One hour before each airway challenge,the TAU group rats and DXM group rats were respectively administered intraperitoneally with TAU 0.5 g/kg and DXM 0.5 mg/kg.Counted the total number of leukocyte and eosinophil number in bronchoalveodar lavage fluid (BALF),determined for airway MLCK by immunohistochemistry,determined for IL-5 levels in blood serum by Elisa.Results ① The expression of MLCK and IL-5 in asthma group increased significantly as compared with normal group (t =4.324,P ＜0.05;t =3.984,P ＜0.05).The expression of MLCK and IL-5 in TAU group decreased significantly as compared with asthma group (t =3.987,P＜0.05;t =4.375,P ＜0.05).The expression of MLCK and IL-5 in TAU group were not changed significantly as compared with DXM group (t =0.780,P ＞0.05;t =0.674,P ＞0.05).②The asthmatic symptoms of TAU group rats and DEX group rats were significantly reduced as compared with asthma group,but the TAU group rats did not lose weight and appetite.Conclusions Taurine could decrease the level of MLCK in the airway in asthma rats to relax airway and reduce airway inflammation.
