国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
7期
504-507
,共4页
黄可%沈瑛%樊满齐%杨伟康%刘翱
黃可%瀋瑛%樊滿齊%楊偉康%劉翱
황가%침영%번만제%양위강%류고
调节性T细胞%慢性阻塞性肺疾病%Th17细胞%流式细胞仪
調節性T細胞%慢性阻塞性肺疾病%Th17細胞%流式細胞儀
조절성T세포%만성조새성폐질병%Th17세포%류식세포의
Regulatory T cell%Chronic obstructive pulmonary disease%Th17 cell%Flow cytometry
目的 探讨CD4+ CD25+ CD127low调节性T细胞(Treg细胞)、Th17细胞特异性分泌因子IL-17在COPD急性加重期(AECOPD)及COPD稳定期中的改变及临床意义.方法 选取住院AECOPD患者26例,随访COPD稳定期患者20例,健康对照患者15例.流式细胞仪检测患者外周血CD4+ CD25+ CD1271.wTreg细胞比例、ELISA检测外周血IL-17浓度.结果 ①AECOPD组患者外周血Treg细胞比例较COPD稳定期组、对照组均明显升高[分别为(10.319±2.154)％,(6.406±1.498)％,(6.307±1.626)％;P＜0.05],COPD稳定期组患者Treg细胞比例高于对照组但差异无统计学意义;②A ECO PD组及COPD稳定期组外周血IL-17水平较对照组明显升高[分别为(0.813±0.233) ng/L,(o.547±0.157) ng/L,(0.408±0.169) ng/L;P＜0.001],AECOPD组IL-17水平高于COPD稳定期组(P＜0.01),COPD稳定期组IL-17水平高于对照组(P=0.046);③各组Treg细胞、IL-17水平与各指标之间未见相关性.结论 Treg细胞、IL-17在AECOPD及COPD稳定期中的水平异常说明在COPD的不同时期存在着不同的炎症,Treg/Th17细胞失衡可能导致COPD的免疫紊乱和慢性炎症持续.
目的 探討CD4+ CD25+ CD127low調節性T細胞(Treg細胞)、Th17細胞特異性分泌因子IL-17在COPD急性加重期(AECOPD)及COPD穩定期中的改變及臨床意義.方法 選取住院AECOPD患者26例,隨訪COPD穩定期患者20例,健康對照患者15例.流式細胞儀檢測患者外週血CD4+ CD25+ CD1271.wTreg細胞比例、ELISA檢測外週血IL-17濃度.結果 ①AECOPD組患者外週血Treg細胞比例較COPD穩定期組、對照組均明顯升高[分彆為(10.319±2.154)％,(6.406±1.498)％,(6.307±1.626)％;P＜0.05],COPD穩定期組患者Treg細胞比例高于對照組但差異無統計學意義;②A ECO PD組及COPD穩定期組外週血IL-17水平較對照組明顯升高[分彆為(0.813±0.233) ng/L,(o.547±0.157) ng/L,(0.408±0.169) ng/L;P＜0.001],AECOPD組IL-17水平高于COPD穩定期組(P＜0.01),COPD穩定期組IL-17水平高于對照組(P=0.046);③各組Treg細胞、IL-17水平與各指標之間未見相關性.結論 Treg細胞、IL-17在AECOPD及COPD穩定期中的水平異常說明在COPD的不同時期存在著不同的炎癥,Treg/Th17細胞失衡可能導緻COPD的免疫紊亂和慢性炎癥持續.
목적 탐토CD4+ CD25+ CD127low조절성T세포(Treg세포)、Th17세포특이성분비인자IL-17재COPD급성가중기(AECOPD)급COPD은정기중적개변급림상의의.방법 선취주원AECOPD환자26례,수방COPD은정기환자20례,건강대조환자15례.류식세포의검측환자외주혈CD4+ CD25+ CD1271.wTreg세포비례、ELISA검측외주혈IL-17농도.결과 ①AECOPD조환자외주혈Treg세포비례교COPD은정기조、대조조균명현승고[분별위(10.319±2.154)％,(6.406±1.498)％,(6.307±1.626)％;P＜0.05],COPD은정기조환자Treg세포비례고우대조조단차이무통계학의의;②A ECO PD조급COPD은정기조외주혈IL-17수평교대조조명현승고[분별위(0.813±0.233) ng/L,(o.547±0.157) ng/L,(0.408±0.169) ng/L;P＜0.001],AECOPD조IL-17수평고우COPD은정기조(P＜0.01),COPD은정기조IL-17수평고우대조조(P=0.046);③각조Treg세포、IL-17수평여각지표지간미견상관성.결론 Treg세포、IL-17재AECOPD급COPD은정기중적수평이상설명재COPD적불동시기존재착불동적염증,Treg/Th17세포실형가능도치COPD적면역문란화만성염증지속.
Objective To explore the change and significance of CD4+ CD25+ CD127low regulatory T cell (Treg cell) and Th17 cell-specific cytokines:interleukin-17 (IL-17) in acute exacerated COPD and stable COPD.Methods Twenty-six inpatients with acute exacerbate COPD (AECOPD),22 follow-up visit patients with stable COPD,and 15 healthy person as control group.Flow cytometry was used to detect the proportion of Treg cell in peripheral boood,ELISA method was used to detect the expresstion of IL-17 in peripheral boood.Results ① The proportion of Treg cell in group AECOPD increased significantly compared with group stable COPD and control group [(10.319 ± 2.154) ％,(6.406 ± 1.498) ％,(6.307± 1.626) ％,P ＜ 0.001].Group stable COPD is higher than control group,but there are no significance.②The level of IL-17 in peripheral boood in group AECOPD and group stable COPD increased significantly compared with control group [(0.813 ± 0.233) ng/L,(0.547 ±-0.157) ng/L,(0.408 ± 0.169) ng/L;P ＜ 0.001],group AECOPD is higher than group COPD (P ＜ 0.01),group COPD is higher than control group (P =0.046).③There was not correlated between the proportion of Treg cell and the level of IL-17 and other indicators in either group.Conclusions The abnormal of lever of regulatory T cell and IL-17 in AECOPD and COPD meant different inflammation may exist in different stage of COPD.The break of balance of Treg cell and Th17 cell may induce the disorder of immune and continue inflammation.
