国际呼吸杂志
國際呼吸雜誌
국제호흡잡지
INTERNATIONAL JOURNAL OF RESPIRATION
2015年
7期
513-516
,共4页
间歇低氧%心肌重塑%丝裂原活化蛋白激酶%心锚重复蛋白%大鼠
間歇低氧%心肌重塑%絲裂原活化蛋白激酶%心錨重複蛋白%大鼠
간헐저양%심기중소%사렬원활화단백격매%심묘중복단백%대서
Intermittent hypoxia%Myocardial remodeling%Mitogen activated protein kinase%Cardiac ankyrin repeat protein%Rat
目的 通过构建间歇低氧大鼠模型,检测模型大鼠心肌肥厚指数、心肌组织中p38MAPK、心锚重复蛋白(CARP)蛋白表达量的变化,探讨间歇低氧对心肌重塑的影响.方法 选取健康雄性SD大鼠24只,随机分3组:间歇低氧8周组(IH8)、间歇低氧4周组(IH4)、常氧组(NC)每组8只.测定各组体质量、左心室质量及左室肥厚指数;用免疫组织化学法测定各组心肌组织p38MAPK、CARP蛋白含量,并做HE染色,观察各组心肌组织形态学变化.结果 与NC组大鼠相比,IH4、IH8组大鼠心肌肥厚指数增加,IH8组更为明显(P＜0.05);IH8、IH4组p38MAPK、CARP蛋白表达均较NC组增加,IH8组较IH4组更为明显(P＜0.001);HE染色结果显示IH4、IH8组心肌细胞排列紊乱,IH8组更为明显.结论 间歇低氧与心肌重塑密切相关,p38MAPK通路与CARP的表达上调可能是其发生机制之一.
目的 通過構建間歇低氧大鼠模型,檢測模型大鼠心肌肥厚指數、心肌組織中p38MAPK、心錨重複蛋白(CARP)蛋白錶達量的變化,探討間歇低氧對心肌重塑的影響.方法 選取健康雄性SD大鼠24隻,隨機分3組:間歇低氧8週組(IH8)、間歇低氧4週組(IH4)、常氧組(NC)每組8隻.測定各組體質量、左心室質量及左室肥厚指數;用免疫組織化學法測定各組心肌組織p38MAPK、CARP蛋白含量,併做HE染色,觀察各組心肌組織形態學變化.結果 與NC組大鼠相比,IH4、IH8組大鼠心肌肥厚指數增加,IH8組更為明顯(P＜0.05);IH8、IH4組p38MAPK、CARP蛋白錶達均較NC組增加,IH8組較IH4組更為明顯(P＜0.001);HE染色結果顯示IH4、IH8組心肌細胞排列紊亂,IH8組更為明顯.結論 間歇低氧與心肌重塑密切相關,p38MAPK通路與CARP的錶達上調可能是其髮生機製之一.
목적 통과구건간헐저양대서모형,검측모형대서심기비후지수、심기조직중p38MAPK、심묘중복단백(CARP)단백표체량적변화,탐토간헐저양대심기중소적영향.방법 선취건강웅성SD대서24지,수궤분3조:간헐저양8주조(IH8)、간헐저양4주조(IH4)、상양조(NC)매조8지.측정각조체질량、좌심실질량급좌실비후지수;용면역조직화학법측정각조심기조직p38MAPK、CARP단백함량,병주HE염색,관찰각조심기조직형태학변화.결과 여NC조대서상비,IH4、IH8조대서심기비후지수증가,IH8조경위명현(P＜0.05);IH8、IH4조p38MAPK、CARP단백표체균교NC조증가,IH8조교IH4조경위명현(P＜0.001);HE염색결과현시IH4、IH8조심기세포배렬문란,IH8조경위명현.결론 간헐저양여심기중소밀절상관,p38MAPK통로여CARP적표체상조가능시기발생궤제지일.
Objective To investigate the relationship of p38MAPK proein and CARP protein with myocardial remodeling in rats processed by intermittent hypoxic in rats.Methods Twenty-four healthy male SD rats were randomly divided into three groups:normal control group (NC group),intermittent hypoxia 4 weeks group (IH4 group),and intermittent hypoxia 8 weeks group (IH8 group),with 8 rats in each group.Left ventricular weight index (LVWI) in all groups were measured on 4 weeks and 8 weeks,and then detect the myocardial changes (HE staining) and expressions of p38MAPK proein and CARP protein with immunohistochemical method.Results Compared with the NC group,LVWI in IH4 and IH8 groups was higher,IH8 group was higher than other groups (P ＜0.05).Compared with the NC group,the expression of p38MAPK proein was increased in IH4 and IH8 groups,IH8 group was higher than other groups (P ＜0.001),the expression of CARP proein was also increased in IH4 and IH8 groups,IH8 group was higher than other groups(P ＜0.001).Conclusions Intermittent hypoxic is closely related to myocardial remodeling,p38MAPK proein and CARP protein expression may be one of the mechanisms.
