中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2015年
4期
241-246
,共6页
石浩%张海娜%高晶晶%蔡丽晓%周磊%李铁山
石浩%張海娜%高晶晶%蔡麗曉%週磊%李鐵山
석호%장해나%고정정%채려효%주뢰%리철산
脑缺血%再灌注%肌萎缩%蛋白激酶B%跑台训练
腦缺血%再灌註%肌萎縮%蛋白激酶B%跑檯訓練
뇌결혈%재관주%기위축%단백격매B%포태훈련
Brain ischemia%Reperfusion%Muscular atrophy%Protein kinase B%Treadmill training
目的 观察不同强度跑台训练对脑缺血再灌注损伤大鼠骨骼肌中磷酸化蛋白激酶B(p-Akt)的表达及运动功能的影响.方法 选取符合纳入标准的120只大鼠,按随机数字法分为假手术组、模型组、普通训练组和强化训练组,每组30只.通过线栓法建立大鼠左侧大脑缺血再灌注(MCAO)损伤模型,假手术组不阻断大脑中动脉,不给于跑台训练;模型组大鼠MCAO造模成功后,未给予跑台训练;普通训练组大鼠MCAO造模成功后,每日给予30 min的跑台训练(普通训练);强化训练组大鼠MCAO造模成功后给予每日早晚各1次的30 min跑台训练(强化训练).分别于训练第1、3、7和14天,采用Zausinger六分法评分检测各组大鼠的神经功能情况并加以比较.于实验第3、7和14天三个时间点,每组各取5只大鼠灌注后分别取两侧腓肠肌行苏木素-伊红(HE)染色,镜下观察各组大鼠腓肠肌纤维横截面形态,镜下每个视野取10个轮廓较为完整的肌纤维,计算每根肌纤维平均横截面积及其横截面积维持率.将各组实验第3、7和14天三个时间点剩余的大鼠取患侧腓肠肌,采用Western Blotting法检测分析其p-Akt蛋白的表达情况.结果 ①给予跑台训练第1、3和7天后,普通训练组和强化训练组的Zausinger行为学评分差异无统计学意义(P＞0.05);但跑台训练第14天后,强化训练组行为学评分明显高于普通训练组(P＜0.05).②跑台训练第3天,各组大鼠腓肠肌横截面积维持率两两比较,差异无统计学意义(P＞0.05);而在跑台训练第7天和第14天时,假手术组大鼠腓肠肌横截面积维持率[(96.67±2.76)％和(94.34±6.17)％]均明显高于同时间点模型组[(70.35±2.21)％和(64.89±2.45)％]、普通训练组[(78.68±3.46)％和(71.39±5.72)％]和强化训练组[(83.31±2.89)％和(78.78±3.67)％],且组间差异均有统计学意义(P＜0.05);而且强化训练组大鼠腓肠肌横截面积维持率均明显大于同时间点的普通训练组(P＜0.05).③跑台训练第7天和第14天,强化训练组p-Akt蛋白表达水平[(0.749±0.042)和(0.689±0.064)]明显高于普通训练组[(0.578±0.072)和(0.433±0.106)],且组间差异有统计学意义(P＜0.05).结论 跑台训练可促进p-Akt蛋白的表达;强化训练比普通训练更加有利于p-Akt蛋白的表达,更能防止肌肉萎缩和改善患侧肢体功能.
目的 觀察不同彊度跑檯訓練對腦缺血再灌註損傷大鼠骨骼肌中燐痠化蛋白激酶B(p-Akt)的錶達及運動功能的影響.方法 選取符閤納入標準的120隻大鼠,按隨機數字法分為假手術組、模型組、普通訓練組和彊化訓練組,每組30隻.通過線栓法建立大鼠左側大腦缺血再灌註(MCAO)損傷模型,假手術組不阻斷大腦中動脈,不給于跑檯訓練;模型組大鼠MCAO造模成功後,未給予跑檯訓練;普通訓練組大鼠MCAO造模成功後,每日給予30 min的跑檯訓練(普通訓練);彊化訓練組大鼠MCAO造模成功後給予每日早晚各1次的30 min跑檯訓練(彊化訓練).分彆于訓練第1、3、7和14天,採用Zausinger六分法評分檢測各組大鼠的神經功能情況併加以比較.于實驗第3、7和14天三箇時間點,每組各取5隻大鼠灌註後分彆取兩側腓腸肌行囌木素-伊紅(HE)染色,鏡下觀察各組大鼠腓腸肌纖維橫截麵形態,鏡下每箇視野取10箇輪廓較為完整的肌纖維,計算每根肌纖維平均橫截麵積及其橫截麵積維持率.將各組實驗第3、7和14天三箇時間點剩餘的大鼠取患側腓腸肌,採用Western Blotting法檢測分析其p-Akt蛋白的錶達情況.結果 ①給予跑檯訓練第1、3和7天後,普通訓練組和彊化訓練組的Zausinger行為學評分差異無統計學意義(P＞0.05);但跑檯訓練第14天後,彊化訓練組行為學評分明顯高于普通訓練組(P＜0.05).②跑檯訓練第3天,各組大鼠腓腸肌橫截麵積維持率兩兩比較,差異無統計學意義(P＞0.05);而在跑檯訓練第7天和第14天時,假手術組大鼠腓腸肌橫截麵積維持率[(96.67±2.76)％和(94.34±6.17)％]均明顯高于同時間點模型組[(70.35±2.21)％和(64.89±2.45)％]、普通訓練組[(78.68±3.46)％和(71.39±5.72)％]和彊化訓練組[(83.31±2.89)％和(78.78±3.67)％],且組間差異均有統計學意義(P＜0.05);而且彊化訓練組大鼠腓腸肌橫截麵積維持率均明顯大于同時間點的普通訓練組(P＜0.05).③跑檯訓練第7天和第14天,彊化訓練組p-Akt蛋白錶達水平[(0.749±0.042)和(0.689±0.064)]明顯高于普通訓練組[(0.578±0.072)和(0.433±0.106)],且組間差異有統計學意義(P＜0.05).結論 跑檯訓練可促進p-Akt蛋白的錶達;彊化訓練比普通訓練更加有利于p-Akt蛋白的錶達,更能防止肌肉萎縮和改善患側肢體功能.
목적 관찰불동강도포태훈련대뇌결혈재관주손상대서골격기중린산화단백격매B(p-Akt)적표체급운동공능적영향.방법 선취부합납입표준적120지대서,안수궤수자법분위가수술조、모형조、보통훈련조화강화훈련조,매조30지.통과선전법건립대서좌측대뇌결혈재관주(MCAO)손상모형,가수술조불조단대뇌중동맥,불급우포태훈련;모형조대서MCAO조모성공후,미급여포태훈련;보통훈련조대서MCAO조모성공후,매일급여30 min적포태훈련(보통훈련);강화훈련조대서MCAO조모성공후급여매일조만각1차적30 min포태훈련(강화훈련).분별우훈련제1、3、7화14천,채용Zausinger륙분법평분검측각조대서적신경공능정황병가이비교.우실험제3、7화14천삼개시간점,매조각취5지대서관주후분별취량측비장기행소목소-이홍(HE)염색,경하관찰각조대서비장기섬유횡절면형태,경하매개시야취10개륜곽교위완정적기섬유,계산매근기섬유평균횡절면적급기횡절면적유지솔.장각조실험제3、7화14천삼개시간점잉여적대서취환측비장기,채용Western Blotting법검측분석기p-Akt단백적표체정황.결과 ①급여포태훈련제1、3화7천후,보통훈련조화강화훈련조적Zausinger행위학평분차이무통계학의의(P＞0.05);단포태훈련제14천후,강화훈련조행위학평분명현고우보통훈련조(P＜0.05).②포태훈련제3천,각조대서비장기횡절면적유지솔량량비교,차이무통계학의의(P＞0.05);이재포태훈련제7천화제14천시,가수술조대서비장기횡절면적유지솔[(96.67±2.76)％화(94.34±6.17)％]균명현고우동시간점모형조[(70.35±2.21)％화(64.89±2.45)％]、보통훈련조[(78.68±3.46)％화(71.39±5.72)％]화강화훈련조[(83.31±2.89)％화(78.78±3.67)％],차조간차이균유통계학의의(P＜0.05);이차강화훈련조대서비장기횡절면적유지솔균명현대우동시간점적보통훈련조(P＜0.05).③포태훈련제7천화제14천,강화훈련조p-Akt단백표체수평[(0.749±0.042)화(0.689±0.064)]명현고우보통훈련조[(0.578±0.072)화(0.433±0.106)],차조간차이유통계학의의(P＜0.05).결론 포태훈련가촉진p-Akt단백적표체;강화훈련비보통훈련경가유리우p-Akt단백적표체,경능방지기육위축화개선환측지체공능.
Objective To observe the effects of treadmill training at different intensities on the expression of p-AKT in rats' gastrocnemius muscles after focal cerebral ischemia,and to investigate whether intensive training is beneficial for the recovery of motor function.Methods Left middle cerebral artery occlusion (MCAO) was induced in 120 male Wistar rats using the intraluminal thread method,and they were divided into an MCAO group (no training),a normal training group (treadmill training once a day for 30 min) and an intensive training group (treadmill training twice a day for 60 min),each of 30 rats.There was also a sham control group with 30 members not given MCAO or training.The four groups were further divided into 3 day,7 day and 14 day subgroups.Five rats randomly selected from each subgroup were sacrificed for hematoxylin-eosin staining after 4％ paraformaldehyde treatment.Neurological function was evaluated using Zausinger scores,and the expression of p-Akt was detected by western blotting.Results No significant differences in Zausinger scores were observed between the intensive training group and the normal training group after 1,3 or 7 days of training.However the average Zausinger score in the intensive training group was significantly higher than in the normal training group after 14 d of treadmill training.After 7 d and 14 d of treadmill training the average cross-sectional area of the gastrocnemius muscles in the sham group was significantly higher than in the other three groups.The average area of the intensive training group was significantly larger than that of the normal training group.The expression of p-Akt in the gastrocnemius was significantly increased in the intensive training group compared with the normal training group in the 7 day and 14 day subgroups.Conclusion Treadmill training can improve the expression of p-Akt in atrophied gastrocnemius muscles caused by MCAO.Intensive training is more effective for the recovery of muscle function.
