中华劳动卫生职业病杂志
中華勞動衛生職業病雜誌
중화노동위생직업병잡지
CHINESE JOURNAL OF INDUSTRIAL HYGIENE AND OCCUPATIONAL DISEASES
2015年
4期
301-303
,共3页
尿%苯乙醇酸%苯乙醛酸%色谱法
尿%苯乙醇痠%苯乙醛痠%色譜法
뇨%분을순산%분을철산%색보법
Urine%Mandelic acid%Phenylglyoxylic acid%Chromatography
目的 建立尿中苯乙醇酸(MA)和苯乙醛酸(PGA)测定的免试剂离子色谱法.方法 利用离子色谱仪,选用AS19阴离子分析柱,10～35 mmol/L KOH梯度淋洗,流速为1.00 ml/min,紫外检测波长:MA 225 nm,PGA 254 nm.样品用纯水10倍稀释后,经银柱去除高浓度氯离子,0.2 μm滤膜过滤后进样.结果 MA和PGA的加标回收率为96.5％～99.3％,相对标准偏差均小于5.0％,在1.0～100.0 mg/L范围均呈现良好的线性关系,回归系数大于0.999 5.MA和PGA检出限分别为0.02和0.05 mg/L;最低检出浓度分别为0.2和0.5 mg/L(取样量为5.0 ml,纯水定容至50.0 ml,进样量为300μl).结论 该方法快速简单,灵敏度高,选择性强,可应用于苯乙烯工作场所工人尿中MA和PGA的检测.
目的 建立尿中苯乙醇痠(MA)和苯乙醛痠(PGA)測定的免試劑離子色譜法.方法 利用離子色譜儀,選用AS19陰離子分析柱,10～35 mmol/L KOH梯度淋洗,流速為1.00 ml/min,紫外檢測波長:MA 225 nm,PGA 254 nm.樣品用純水10倍稀釋後,經銀柱去除高濃度氯離子,0.2 μm濾膜過濾後進樣.結果 MA和PGA的加標迴收率為96.5％～99.3％,相對標準偏差均小于5.0％,在1.0～100.0 mg/L範圍均呈現良好的線性關繫,迴歸繫數大于0.999 5.MA和PGA檢齣限分彆為0.02和0.05 mg/L;最低檢齣濃度分彆為0.2和0.5 mg/L(取樣量為5.0 ml,純水定容至50.0 ml,進樣量為300μl).結論 該方法快速簡單,靈敏度高,選擇性彊,可應用于苯乙烯工作場所工人尿中MA和PGA的檢測.
목적 건립뇨중분을순산(MA)화분을철산(PGA)측정적면시제리자색보법.방법 이용리자색보의,선용AS19음리자분석주,10～35 mmol/L KOH제도림세,류속위1.00 ml/min,자외검측파장:MA 225 nm,PGA 254 nm.양품용순수10배희석후,경은주거제고농도록리자,0.2 μm려막과려후진양.결과 MA화PGA적가표회수솔위96.5％～99.3％,상대표준편차균소우5.0％,재1.0～100.0 mg/L범위균정현량호적선성관계,회귀계수대우0.999 5.MA화PGA검출한분별위0.02화0.05 mg/L;최저검출농도분별위0.2화0.5 mg/L(취양량위5.0 ml,순수정용지50.0 ml,진양량위300μl).결론 해방법쾌속간단,령민도고,선택성강,가응용우분을희공작장소공인뇨중MA화PGA적검측.
Objective To develop a method for determination of mandelic acid (MA) and phenylglyoxylic acid (PGA) in urine by reagent-free ion chromatography.Methods Ion chromatography was performed on an AS19 column with a gradient elution solution containing 10～35 mmol/L KOH at a flow rate of 1.00 ml/min,and MA and PGA were detected at ultraviolet wavelengths of 225 nm and 254 nm,respectively.The samples were diluted 10 times with purified water,then purified on a silver column to remove high concentrations of chloride ion,and injected after being filtered through a 0.2-μm filter membrane.Results The recoveries of standard addition of MA and PGA were 96.5％ and 99.3％,respectively,with both relative standard deviations less than 5.0％.Good linear relationships were noted in the range of 1.0～100.0 mg/L for both MA and PGA (r ＞0.9995).The detection limits of MA and PGA were 0.02 mg/L and 0.05 mg/L,respectively;the minimum detectable concentrations of MA and PGA were 0.2 mg/L and 0.5 mg/L (when the sampling amount was 5.0 ml and diluted to 50.0 ml with water,and the injection volume was 300 μL).Conclusions This method is fast,convenient,and highly sensitive and selective.It can be used for the analysis of MA and PGA in the urine of styreneexposed workers.
