中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2015年
14期
60-62,63
,共4页
结肠肿瘤%DNA甲基化%组织%干扰素-γ%白细胞介素-10
結腸腫瘤%DNA甲基化%組織%榦擾素-γ%白細胞介素-10
결장종류%DNA갑기화%조직%간우소-γ%백세포개소-10
Colon cancer%DNA methylation%Tissue%Interferon-γ%Interleukin-10
目的:研究结肠癌组织白细胞介素-10(IL-10)和干扰素-γ(IFN-γ)的基因表达及其相关的启动子区甲基化状态。方法:随机选取2011年1月-2014年6月80例结肠癌患者组织为结肠癌组、80例相应癌旁组织为癌旁组和100例正常健康者结肠组织为对照组。采用亚硫酸氢盐测序法、甲基化特异性PCR和实时荧光定量PCR检测对IL-10和IFN-γ基因的甲基化状况进行检测,分析其与结肠癌主要临床特征之间的关系。结果:三组组织的IL-10和IFN-γ mRNA 水平之间比较,差异均有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10 mRNA均高于对照组(P<0.05),结肠癌组和癌旁组组织的IFN-γ mRNA均低于对照组(P<0.05);三组IL-10基因启动子区-110,-185位点甲基化率比较,差异均有有统计学意义(P<0.05),其中结肠癌组和癌旁组组织的IL-10基因启动子区-110均高于对照组(P<0.05),结肠癌组组织的IL-10基因启动子区-185低于对照组(P<0.05);IFN-γ基因启动子区甲基化率结肠癌组>癌旁组>对照组,差异均有统计学意义(P<0.05)。结论:IL-10和IFN-γ基因启动子区甲基化状态影响其在结肠癌组织中的表达,结肠癌患者处于TH2极化状态,与结肠癌发生发展密切相关。
目的:研究結腸癌組織白細胞介素-10(IL-10)和榦擾素-γ(IFN-γ)的基因錶達及其相關的啟動子區甲基化狀態。方法:隨機選取2011年1月-2014年6月80例結腸癌患者組織為結腸癌組、80例相應癌徬組織為癌徬組和100例正常健康者結腸組織為對照組。採用亞硫痠氫鹽測序法、甲基化特異性PCR和實時熒光定量PCR檢測對IL-10和IFN-γ基因的甲基化狀況進行檢測,分析其與結腸癌主要臨床特徵之間的關繫。結果:三組組織的IL-10和IFN-γ mRNA 水平之間比較,差異均有統計學意義(P<0.05),其中結腸癌組和癌徬組組織的IL-10 mRNA均高于對照組(P<0.05),結腸癌組和癌徬組組織的IFN-γ mRNA均低于對照組(P<0.05);三組IL-10基因啟動子區-110,-185位點甲基化率比較,差異均有有統計學意義(P<0.05),其中結腸癌組和癌徬組組織的IL-10基因啟動子區-110均高于對照組(P<0.05),結腸癌組組織的IL-10基因啟動子區-185低于對照組(P<0.05);IFN-γ基因啟動子區甲基化率結腸癌組>癌徬組>對照組,差異均有統計學意義(P<0.05)。結論:IL-10和IFN-γ基因啟動子區甲基化狀態影響其在結腸癌組織中的錶達,結腸癌患者處于TH2極化狀態,與結腸癌髮生髮展密切相關。
목적:연구결장암조직백세포개소-10(IL-10)화간우소-γ(IFN-γ)적기인표체급기상관적계동자구갑기화상태。방법:수궤선취2011년1월-2014년6월80례결장암환자조직위결장암조、80례상응암방조직위암방조화100례정상건강자결장조직위대조조。채용아류산경염측서법、갑기화특이성PCR화실시형광정량PCR검측대IL-10화IFN-γ기인적갑기화상황진행검측,분석기여결장암주요림상특정지간적관계。결과:삼조조직적IL-10화IFN-γ mRNA 수평지간비교,차이균유통계학의의(P<0.05),기중결장암조화암방조조직적IL-10 mRNA균고우대조조(P<0.05),결장암조화암방조조직적IFN-γ mRNA균저우대조조(P<0.05);삼조IL-10기인계동자구-110,-185위점갑기화솔비교,차이균유유통계학의의(P<0.05),기중결장암조화암방조조직적IL-10기인계동자구-110균고우대조조(P<0.05),결장암조조직적IL-10기인계동자구-185저우대조조(P<0.05);IFN-γ기인계동자구갑기화솔결장암조>암방조>대조조,차이균유통계학의의(P<0.05)。결론:IL-10화IFN-γ기인계동자구갑기화상태영향기재결장암조직중적표체,결장암환자처우TH2겁화상태,여결장암발생발전밀절상관。
Objective:To investigate the gene expression of interleukin-10(IL-10) and interferon-γ(IFN-γ) colon tissue and methylation status of the promoter region.Method:Randomly selected from January 2011 to June 2014 colon cancer patient’s tissue 80 cases of colon cancer group, 80 cases of corresponding adjacent tissues as adjacent group, ad 100 cases of normal healthy colon tissue as the control group.Using bisulfite sequencing method (BSP),methylation specific PCR(MSP) and real-time quantitative PCR (RT-PCR) detection of promoter methylation of three groups of patients with colonic tissue IL-10 and IFN-γ gene and mRNA expression status.Result:Comparison of the three groups in the tissue IL-10 and IFN-γ mRNA, the differences were statistically significant(P<0.05).Colon cancer group and adjacent group of IL-10 mRNA were higher than the control group(P<0.05),IFN-γ mRNA were lower than in the control group(P<0.05).Three groups of IL-10 gene promoter region-110,-185 sites methylation rates,the difference was statistically significant(P<0.05).Colon cancer group and adjacent group organization of IL-10 gene promoter region -110 were higher than the control group(P<0.05),colon cancer group IL-10 gene promoter region-185 were lower than in the control group(P<0.05);IFN-γ gene promoter methylation of the colon cancer group>the adjacent group>the control group, the differences were statistically significant(P<0.05).Conclusion:IL-10 and IFN-γ gene promoter methylation status affect its expression in colon cancer, colon cancer patients in TH2 polarization state, closely related to the development of colon cancer.