中华实用儿科临床杂志
中華實用兒科臨床雜誌
중화실용인과림상잡지
Journal of Applied Clinical Pediatrics
2015年
7期
521-524
,共4页
王钢强%李长钢%王国兵%黄美玲
王鋼彊%李長鋼%王國兵%黃美玲
왕강강%리장강%왕국병%황미령
免疫性血小板减少症%调节性T淋巴细胞%白细胞相关免疫球蛋白样受体%儿童%免疫失调
免疫性血小闆減少癥%調節性T淋巴細胞%白細胞相關免疫毬蛋白樣受體%兒童%免疫失調
면역성혈소판감소증%조절성T림파세포%백세포상관면역구단백양수체%인동%면역실조
Immune thrombocytopenia%Regulatory T cell%Leukocyte-associated immunoglobulin-like receptor%Child%Immune disorders
目的 观察免疫性血小板减少症(ITP)患儿调节性T淋巴细胞(Treg细胞)白细胞相关免疫球蛋白样受体-1(LAIR-1)的表达情况及外周血可溶性LAIR-1(sLAIR-1)、LAIR-2水平,探讨其在ITP发病中的机制.方法 应用流式细胞术检测初发未经治疗ITP患儿外周血Treg细胞的比例及LAIR-1在其膜表面的表达率;应用酶联免疫吸附试验(ELISA)法检测36例ITP患儿血浆中sLAIR-1及LAIR-2的水平;应用实时荧光定量(RT)-PCR法检测ITP患儿LAIR-1、LAIR-2 mRNA表达水平.28例同龄健康儿童作为健康对照组.结果 ITP患儿组Treg细胞表达比率低于健康对照组,差异有统计学意义[(2.05±0.85)％比(3.04±1.03)％,t=4.198,P＜0.001];ITP组Treg细胞膜LAIR-1和酪氨酸磷酸酶2(SHP-2)表达率与健康对照组比较差异无统计学意义[(71.18±13.36)％比(67.69 ±13.07)％,t=1.045,P＞0.05;(1.20±0.97)％比(0.85±0.66)％;t=1.718,P＞0.05];ITP组血浆中sLAIR-1及LAIR-2水平均高于健康对照组[(20.53±4.32)μg/L比(17.51±5.15) μg/L,t=2.424,P＜0.05;(5.83±1.08)μg/L比(5.19±1.24) μg/L,t=2.267,P＜0.05],LAIR-1mRNA表达水平高于健康对照组(t=2.851,P＜0.05),而LAIR-2 mRNA表达水平2组差异无统计学意义(t=1.715,P＞0.05).结论 ITP患儿Treg细胞表达降低,可能与ITP发病有关;在ITP发生过程中,LAIR-1对Treg细胞表达变化未起到主导作用,血浆sLAIR-1、LAIR-2水平升高,LAIR-1、LAIR-2可能是ITP患儿免疫失调的因素之一.
目的 觀察免疫性血小闆減少癥(ITP)患兒調節性T淋巴細胞(Treg細胞)白細胞相關免疫毬蛋白樣受體-1(LAIR-1)的錶達情況及外週血可溶性LAIR-1(sLAIR-1)、LAIR-2水平,探討其在ITP髮病中的機製.方法 應用流式細胞術檢測初髮未經治療ITP患兒外週血Treg細胞的比例及LAIR-1在其膜錶麵的錶達率;應用酶聯免疫吸附試驗(ELISA)法檢測36例ITP患兒血漿中sLAIR-1及LAIR-2的水平;應用實時熒光定量(RT)-PCR法檢測ITP患兒LAIR-1、LAIR-2 mRNA錶達水平.28例同齡健康兒童作為健康對照組.結果 ITP患兒組Treg細胞錶達比率低于健康對照組,差異有統計學意義[(2.05±0.85)％比(3.04±1.03)％,t=4.198,P＜0.001];ITP組Treg細胞膜LAIR-1和酪氨痠燐痠酶2(SHP-2)錶達率與健康對照組比較差異無統計學意義[(71.18±13.36)％比(67.69 ±13.07)％,t=1.045,P＞0.05;(1.20±0.97)％比(0.85±0.66)％;t=1.718,P＞0.05];ITP組血漿中sLAIR-1及LAIR-2水平均高于健康對照組[(20.53±4.32)μg/L比(17.51±5.15) μg/L,t=2.424,P＜0.05;(5.83±1.08)μg/L比(5.19±1.24) μg/L,t=2.267,P＜0.05],LAIR-1mRNA錶達水平高于健康對照組(t=2.851,P＜0.05),而LAIR-2 mRNA錶達水平2組差異無統計學意義(t=1.715,P＞0.05).結論 ITP患兒Treg細胞錶達降低,可能與ITP髮病有關;在ITP髮生過程中,LAIR-1對Treg細胞錶達變化未起到主導作用,血漿sLAIR-1、LAIR-2水平升高,LAIR-1、LAIR-2可能是ITP患兒免疫失調的因素之一.
목적 관찰면역성혈소판감소증(ITP)환인조절성T림파세포(Treg세포)백세포상관면역구단백양수체-1(LAIR-1)적표체정황급외주혈가용성LAIR-1(sLAIR-1)、LAIR-2수평,탐토기재ITP발병중적궤제.방법 응용류식세포술검측초발미경치료ITP환인외주혈Treg세포적비례급LAIR-1재기막표면적표체솔;응용매련면역흡부시험(ELISA)법검측36례ITP환인혈장중sLAIR-1급LAIR-2적수평;응용실시형광정량(RT)-PCR법검측ITP환인LAIR-1、LAIR-2 mRNA표체수평.28례동령건강인동작위건강대조조.결과 ITP환인조Treg세포표체비솔저우건강대조조,차이유통계학의의[(2.05±0.85)％비(3.04±1.03)％,t=4.198,P＜0.001];ITP조Treg세포막LAIR-1화락안산린산매2(SHP-2)표체솔여건강대조조비교차이무통계학의의[(71.18±13.36)％비(67.69 ±13.07)％,t=1.045,P＞0.05;(1.20±0.97)％비(0.85±0.66)％;t=1.718,P＞0.05];ITP조혈장중sLAIR-1급LAIR-2수평균고우건강대조조[(20.53±4.32)μg/L비(17.51±5.15) μg/L,t=2.424,P＜0.05;(5.83±1.08)μg/L비(5.19±1.24) μg/L,t=2.267,P＜0.05],LAIR-1mRNA표체수평고우건강대조조(t=2.851,P＜0.05),이LAIR-2 mRNA표체수평2조차이무통계학의의(t=1.715,P＞0.05).결론 ITP환인Treg세포표체강저,가능여ITP발병유관;재ITP발생과정중,LAIR-1대Treg세포표체변화미기도주도작용,혈장sLAIR-1、LAIR-2수평승고,LAIR-1、LAIR-2가능시ITP환인면역실조적인소지일.
Objective To observe the expression of leukocyte-associated immunoglobulin(Ig)-like receptor-1 (LAIR-1) on Treg cells in children with immune thrombocytopenia (ITP) and the level of soluble LAIR-1 (sLAIR-1),LAIR-2 in peripheral blood,and to discuss the possible role of LAIR in the pathogenesis of childhood ITP.Methods The levels of LAIR-1 on Treg cells of peripheral blood were measured in 36 children with ITP by using flow cytometry.Plasma levels of sLAIR-1 and LAIR-2 were measured by adopting enzyme-linked immunosorbent assay(ELISA).Real-time PCR was used to measure both LAIR-1 mRNA and LAIR-2 mRNA.Twenty-eight healthy children served as the healthy control group.Results The expression of Treg cells in children with ITP was significantly lower than that in the healthy control group [(2.05 ± 0.85) ％ vs (3.04 ± 1.03) ％,t =4.198,P ＜ 0.001].The expression rate of LAIR-1 on Treg cells and tyrosine phosphatase-2 (SHP-2) in children with ITP group had no statistically significant difference with the healthy control group [(71.18 ± 13.36) ％ vs (67.69 ± 13.07)％,t=1.045,P＞0.05;(1.20 ± 0.97) ％ vs (0.85 ±0.66)％;t=1.718,P＞0.05].The levels of sLAIR-1 and LAIR-2 in plasma in children with ITP group were increased significantly than those in the healthy control group [(20.53 ±4.32) μg/L vs (17.51 ± 5.15) μg/L,t =2.424,P ＜0.05;(5.83 ± 1.08) μg/L vs (5.19 ± 1.24) μg/L,t =2.267,P ＜ 0.05].The LAIR-1 mRNA expression level in children with ITP group was significantly increased compared with the healthy control group (t =2.851,P ＜ 0.05),but not the LAIR-2 mRNA expression level (t =1.715,P ＞ 0.05).Conclusions The expression of Treg cells in children with ITP is decreased,and it may be associated with the onset of ITP,and it may suggest that LAIR-1 does not play a leading role in Treg cells when ITP occurrs.And the levels of sLAIR-1,LAIR-2 in plasma are both increased,suggesting that LAIR-1,LAIR-2 may be one of the factors of immune disorders in children with ITP.
