CAJ | 학술논문

目的探讨褪黑素对缺氧缺血损伤后的未成熟脑白质的干预作用及其可能机制.方法 48只3日龄SD大鼠随机数字表法分为假手术组(SHAM组)、缺氧缺血组(HI组)和褪黑素干预组(MT组).参照Rice模型制作脑室周围白质损伤(PWMD)动物模型,MT组分别于术前、术后、术后1h及术后24 h按10 mg/kg腹腔注射褪黑素溶液,SHAM组、HI组则在相同时间点腹腔注射等体积溶剂.分别于术后2d、14 d处死大鼠,断头取脑,应用HE染色、免疫组织化学法染色观察鼠脑脑室周围白质.结果术后2d3组大鼠脑室周围白质组织学结构区别明显,O4阳性细胞数目在SHAM组[(75.548±7.333)/hpf]、MT组[(59.971±3.635)/hpf]、HI组[(40.511±2.848)/hpf]依次减少,差异有统计学意义(P＜0.05);Caspase-3的表达在SHAM组(107.724±10.266)、MT组(132.289±8.537)、HI组(202.168±14.367)依次增加,差异有统计学意义(P＜0.05);术后14 d大鼠手术侧脑室指数在SHAM组(0.928±0.063)、MT组(1.813 ±0.110)、HI组(2.752±0.201)依次增大,髓鞘碱性蛋白(MBP)吸光度值则依次减少,分别为39.504±1.673,21.729±1.614,11.344±1.118.结论 MT可能通过抑制少突胶质细胞前体的凋亡对未成熟大鼠脑PWMD发挥保护作用.
목적 탐토퇴흑소대결양결혈손상후적미성숙뇌백질적간예작용급기가능궤제.방법 48지3일령SD대서수궤수자표법분위가수술조(SHAM조)、결양결혈조(HI조)화퇴흑소간예조(MT조).삼조Rice모형제작뇌실주위백질손상(PWMD)동물모형,MT조분별우술전、술후、술후1h급술후24 h안10 mg/kg복강주사퇴흑소용액,SHAM조、HI조칙재상동시간점복강주사등체적용제.분별우술후2d、14 d처사대서,단두취뇌,응용HE염색、면역조직화학법염색관찰서뇌뇌실주위백질.결과 술후2d3조대서뇌실주위백질조직학결구구별명현,O4양성세포수목재SHAM조[(75.548±7.333)/hpf]、MT조[(59.971±3.635)/hpf]、HI조[(40.511±2.848)/hpf]의차감소,차이유통계학의의(P＜0.05);Caspase-3적표체재SHAM조(107.724±10.266)、MT조(132.289±8.537)、HI조(202.168±14.367)의차증가,차이유통계학의의(P＜0.05);술후14 d대서수술측뇌실지수재SHAM조(0.928±0.063)、MT조(1.813 ±0.110)、HI조(2.752±0.201)의차증대,수초감성단백(MBP)흡광도치칙의차감소,분별위39.504±1.673,21.729±1.614,11.344±1.118.결론 MT가능통과억제소돌효질세포전체적조망대미성숙대서뇌PWMD발휘보호작용.
Objective To investigate the protective effect of melatonin and its possible mechanism for repairing in the immature white matter damage due to brain hypoxia-ischemia (HI).Methods Forty-eight three-day SD rats after birth were randomly divided into 3 groups:sham-operated(SHAM) group,HI group and melatonin treatment(MT) group.Periventricular white matter damage (PWMD) to animal models were estabished according to Rice modeling.MT group was treated with melatonin pre-operatively,immediately postoperation,1 hour postoperation and 24 hours postoperation via intraperitoneal injection,and the other groups were injected with the same volume of dissolvent.The rats were executed by decollation after 2 days and 14 days.The histological changes in periventricular white matter were observed by HE staining and immunohistochemistry.Results For the 3 groups,the structure in ope-ration side of the white matter in the peripheral ventricles of the brain 2 days postoperation were significant different (P ＜0.05).The O4 positive cells decreased one by one/greatest in the SHAM group[(75.548 ± 7.333)/hpf] followed by MT group [(59.971 ± 3.635)/hpf],and HI group [(40.511 ± 2.848)/hpf] (P ＜ 0.05).The expression of Casepase-3 increased in the SHAM group (107.724 ± 10.266),MT group (132.289 ± 8.537),and HI group (202.168 ± 14.367),and the difference was statically significant (P ＜ 0.05).Ventricular index was greater in operation side of the white matter in the peripheral ventricles of the 14-day-brain in the SHAM group(0.928 ±0.063),MT group (1.813 ± 0.110),HI group (2.752 ± 0.201),increasingly,while absorbance value of myelin basic protein decreased one by one in sequence(39.504 ± 1.673,21.729 ± 1.614,11.344 ± 1.118).Conclusions MT plays a role in protecting the periventricular white matter via inhibiting the apoptosis of oligodendrocyte progenitor cell,and thus benefits the PWMD.