CAJ | 학술논문

目的研究职业暴露柴油机尾气(diesel exhaust,DE)对人群细胞增殖能力和基因组稳定性的影响.方法采用整群抽样法于2012年选取117名DE职业暴露工人为暴露组,106名自来水厂工人为对照组,通过问卷调查获得研究对象的人口学资料,并检测工作环境中PM2.5和总多环芳烃(polycyclic aromatic hydrocarbons,PAHs)浓度.采用高效液相色谱-质谱联用方法检测尿中主要PAHs单羟基代谢产物,反映内暴露水平;采用胞质阻滞微核组学试验评价DE对人群外周血淋巴细胞增殖能力和基因组稳定性的影响.结果暴露组和对照组工人尿中总PAHs单羟基代谢产物中位数(P5～P95)分别是12.96 (4.73 ～ 28.10)、4.76(0.90～15.00) μg/L,暴露组高于对照组(Z=-8.77,P＜0.001).暴露组和对照组核分裂指数(nuclear division index,NDI)分别为1.68±0.13、1.85 ±0.16,暴露组降低(t=8.86,P＜0.001),由微核、核质桥和核芽计算所得的基因组不稳定指数,暴露组和对照组分别为13.27±6.26、4.83±3.38,暴露组较高(Z=-10.08,P＜0.001).按尿中总PAHs单羟基代谢产物三分位数将所有研究对象分为低、中、高三组(＜5.96、5.96 ～ 12.46、＞ 12.46 μg/L),随着尿中总多环芳烃羟基代谢物升高,NDI降低,分别为1.81±0.17、1.79±0.17、1.68±0.14(F=13.14,P＜0.001),每1 000个细胞中基因组不稳定指数升高,分别为5.80±4.15、9.97±7.14、11.99±6.61(x2=36.74,P＜0.001).NDI与每1 000个细胞中的微核率、核质桥率、核芽率以及基因组不稳定指数,均随NDI的降低而升高,差异有统计学意义(P ＜0.001).结论职业暴露DE可抑制人群外周血淋巴细胞增殖分裂能力,引起基因组不稳定性增高.
목적 연구직업폭로시유궤미기(diesel exhaust,DE)대인군세포증식능력화기인조은정성적영향.방법 채용정군추양법우2012년선취117명DE직업폭로공인위폭로조,106명자래수엄공인위대조조,통과문권조사획득연구대상적인구학자료,병검측공작배경중PM2.5화총다배방경(polycyclic aromatic hydrocarbons,PAHs)농도.채용고효액상색보-질보련용방법검측뇨중주요PAHs단간기대사산물,반영내폭로수평;채용포질조체미핵조학시험평개DE대인군외주혈림파세포증식능력화기인조은정성적영향.결과 폭로조화대조조공인뇨중총PAHs단간기대사산물중위수(P5～P95)분별시12.96 (4.73 ～ 28.10)、4.76(0.90～15.00) μg/L,폭로조고우대조조(Z=-8.77,P＜0.001).폭로조화대조조핵분렬지수(nuclear division index,NDI)분별위1.68±0.13、1.85 ±0.16,폭로조강저(t=8.86,P＜0.001),유미핵、핵질교화핵아계산소득적기인조불은정지수,폭로조화대조조분별위13.27±6.26、4.83±3.38,폭로조교고(Z=-10.08,P＜0.001).안뇨중총PAHs단간기대사산물삼분위수장소유연구대상분위저、중、고삼조(＜5.96、5.96 ～ 12.46、＞ 12.46 μg/L),수착뇨중총다배방경간기대사물승고,NDI강저,분별위1.81±0.17、1.79±0.17、1.68±0.14(F=13.14,P＜0.001),매1 000개세포중기인조불은정지수승고,분별위5.80±4.15、9.97±7.14、11.99±6.61(x2=36.74,P＜0.001).NDI여매1 000개세포중적미핵솔、핵질교솔、핵아솔이급기인조불은정지수,균수NDI적강저이승고,차이유통계학의의(P ＜0.001).결론 직업폭로DE가억제인군외주혈림파세포증식분렬능력,인기기인조불은정성증고.
Objective To investigate the cell proliferation and genome stability in workers with occupational exposure to diesel exhaust (DE).Methods In 2012,117 DE-exposed workers and 106 control workers were recruited by cluster sampling in this study.The demographic data were obtained by questionnaire survey.The airborne fine panicle and enriched polycyclic aromatic hydrocarbons (PAHs) at different workplaces were collected and analyzed.The concentrations of main PAHs monohydroxy metabolites in the urine were determined by high performance liquid chromatography-mass spectrometry (LC-MS),which could reflect the internal exposure level of DE.The cell proliferation capacity and genome stability in the periphery lymphocytes of workers were evaluated by cytokinesis-block micronucleus (CBMN) cytome assay.Results The concentrations (median (P5-P95)) of total PAHs monohydroxy metabolites in the urine of exposed group and control group were 12.96 (4.73-28.10),4.76 (0.90-15.00) μg/L,respectively,and the exposed group was higher than that of controls (Z =-8.77,P ＜ 0.001).The nuclear division index (NDI) of exposed group and control group was 1.68 ±0.13,1.85 ±0.16,respectively,and the NDI of exposed group showed significantly decreased (t =8.86,P ＜ 0.001),while the genome instability index calculated by micronucleus,nuclear bridges and nuclear buds,of exposed group and control group was 13.27 ± 6.26,4.83 ± 3.38,respectively,and the exposed group had statistically significant increase (Z =-10.08,P ＜0.001).The tertiles of total PAHs monohydroxy metabolites in the urine were categorized into low,medium and high groups(＜5.96,5.96-12.46,＞ 12.46 μg/L).With the NDI decreased,1.81 ± 0.17,1.79 ± 0.17,1.68 ± 0.14 (F =13.14,P ＜ 0.001),genome instability index began to increase 5.80 ± 4.15,9.97 ± 7.14,11.99 ± 6.61 (/1 000),respectively (x2 =36.74,P ＜ 0.001).With the increase of total PAHs monohydroxy metabolites level in corresponding groups.In addition,the NDI was negatively correlated with the frequencies of micronucleus,nuclear bridges,nuclear buds and genome instability index,respectively,and the difference was statistically significant (P ＜ 0.001).Conclusions DE exposure lead to inhibition of cell proliferation capacity and increase genome instability in the peripheral lymphocytes of occupational-exposed population,providing important clues and evidence for early biomarkers monitoring.