中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2015年
3期
233-236
,共4页
陈海丽%周海健%宰淑蓓%蔡金凤%钱方兴%马亮%王茉婴%沈震%李杨
陳海麗%週海健%宰淑蓓%蔡金鳳%錢方興%馬亮%王茉嬰%瀋震%李楊
진해려%주해건%재숙배%채금봉%전방흥%마량%왕말영%침진%리양
弧菌,副溶血性%电泳,凝胶,脉冲场%多位点序列分型
弧菌,副溶血性%電泳,凝膠,脈遲場%多位點序列分型
호균,부용혈성%전영,응효,맥충장%다위점서렬분형
Vibrio parahaemolyticus%Electrophoresis,gel,pulsed-field%Multilocus sequence typing
目的 分析2010-2012年上海市两家哨点医院肠道门诊腹泻病例副溶血弧菌感染情况,以及菌株携带毒力基因状况和分子特征.方法 于2010-2012年,以上海市两家医院的肠道门诊为监测哨点,以医院的2 729例腹泻病例为研究对象,采集每例1份粪便样本.采用硫代硫酸盐柠檬酸盐胆盐蔗糖(TCBS)琼脂培养基及生化反应分离和鉴定副溶血弧菌,采用多重PCR检测分离菌株的毒力基因携带情况,采用PFGE对菌株进行聚类分析,同时进行多位点序列分型(MLST).结果 2 729份样本中,分离到副溶血弧菌30株,2010-2012年的分离率分别为1.1％ (11/973)、1.0％(11/1 120)、1.3％(8/636).携带tlh、tdh、trh基因的菌株分别占100％(30/30)、97％(29/30)、0.30株副溶血弧菌临床分离株共分为13种PFGE型(P1～ 13)和3种MLST型(ST-189、ST-799、ST-3).其中,P4型菌株占30％(9/30),P9、P10型均占12％(4/30),P1、P2、P12、P13型均占7％(2/30),其余PFGE型均占3％(1/30);ST-3型占84％(25/30),ST-189型占13％(4/30),ST-799型占3％(1/30).结论 2010-2012年上海市两家哨点医院腹泻病例副溶血弧菌感染率较低;副溶血弧菌临床分离株均携带tlh基因,不携带trh基因,MLST型以ST-3型为主.
目的 分析2010-2012年上海市兩傢哨點醫院腸道門診腹瀉病例副溶血弧菌感染情況,以及菌株攜帶毒力基因狀況和分子特徵.方法 于2010-2012年,以上海市兩傢醫院的腸道門診為鑑測哨點,以醫院的2 729例腹瀉病例為研究對象,採集每例1份糞便樣本.採用硫代硫痠鹽檸檬痠鹽膽鹽蔗糖(TCBS)瓊脂培養基及生化反應分離和鑒定副溶血弧菌,採用多重PCR檢測分離菌株的毒力基因攜帶情況,採用PFGE對菌株進行聚類分析,同時進行多位點序列分型(MLST).結果 2 729份樣本中,分離到副溶血弧菌30株,2010-2012年的分離率分彆為1.1％ (11/973)、1.0％(11/1 120)、1.3％(8/636).攜帶tlh、tdh、trh基因的菌株分彆佔100％(30/30)、97％(29/30)、0.30株副溶血弧菌臨床分離株共分為13種PFGE型(P1～ 13)和3種MLST型(ST-189、ST-799、ST-3).其中,P4型菌株佔30％(9/30),P9、P10型均佔12％(4/30),P1、P2、P12、P13型均佔7％(2/30),其餘PFGE型均佔3％(1/30);ST-3型佔84％(25/30),ST-189型佔13％(4/30),ST-799型佔3％(1/30).結論 2010-2012年上海市兩傢哨點醫院腹瀉病例副溶血弧菌感染率較低;副溶血弧菌臨床分離株均攜帶tlh基因,不攜帶trh基因,MLST型以ST-3型為主.
목적 분석2010-2012년상해시량가초점의원장도문진복사병례부용혈호균감염정황,이급균주휴대독력기인상황화분자특정.방법 우2010-2012년,이상해시량가의원적장도문진위감측초점,이의원적2 729례복사병례위연구대상,채집매례1빈분편양본.채용류대류산염저몽산염담염자당(TCBS)경지배양기급생화반응분리화감정부용혈호균,채용다중PCR검측분리균주적독력기인휴대정황,채용PFGE대균주진행취류분석,동시진행다위점서렬분형(MLST).결과 2 729빈양본중,분리도부용혈호균30주,2010-2012년적분리솔분별위1.1％ (11/973)、1.0％(11/1 120)、1.3％(8/636).휴대tlh、tdh、trh기인적균주분별점100％(30/30)、97％(29/30)、0.30주부용혈호균림상분리주공분위13충PFGE형(P1～ 13)화3충MLST형(ST-189、ST-799、ST-3).기중,P4형균주점30％(9/30),P9、P10형균점12％(4/30),P1、P2、P12、P13형균점7％(2/30),기여PFGE형균점3％(1/30);ST-3형점84％(25/30),ST-189형점13％(4/30),ST-799형점3％(1/30).결론 2010-2012년상해시량가초점의원복사병례부용혈호균감염솔교저;부용혈호균림상분리주균휴대tlh기인,불휴대trh기인,MLST형이ST-3형위주.
Objective To analyze virulence genes and molecular characteristics of Vibrio parahaemolyticus isolated from sporadic cases with diarrhea in tow sentinel hospitals of Shanghai,2010-2012.Methods A total of 2 729 stool samples were collected from two surveillance sentinel hospitals in Shanghai 2010-2012.Vibrio parahaemolyticus strains were isolated and identified from diarrhea out patients using TCBS agar plates and biochemical reactions.Thermostable direct hemolysingene(tdh),thermostablerelated hemolysin gene (trh),hemolysin gene (tlh) were detected by multiplex PCR method.Isolates were analyzed by PFGE and MLST.The PFGE profiles were analyzed using BioNumerics software.Results A total of 30 clinical Vibrio parahaemolyticus strains isolated from 2 729 stool samples.The anually Vibrio parahaemolyticus isolation rate during 2010 to 2012 were 1.1％ (11/973),1.0％ (11/1 120) and 1.3％ (8/ 636) respectively.The PCR positive rates of virulence genes tlh,tdh and trh were 100％,97％ and 0 respectively.The Vibrio parahaemolyticus strains were divided into 13 PFGE types (P1-P13) and 3 ST types(ST-189,ST-799,ST-3).Among 13 PFGE types,P4 was the main PFGE type,accounting for 30％ (9/30).P9,P10 were accounting for 12％ (4/30) respectively,P1,P2,P12,P13 were accounting for 7％ (2/30)respectively,the others types were 3％ (1/30)respectively.MLST analysis results showed there are three ST types,ST3 was 84％ (25/30),ST189 and ST799 were accounting for 13％ (4/30) and 3％ (1/ 30) respectively.Conclusion The infection rate of Vibrio parahaemolyticus was not very high from 2010-2012 in Shanghai,all strains were positive for tlh and negative for trh.ST3 was the major type of Vibrio parahaemolyticus.
