中华损伤与修复杂志（电子版）
中華損傷與脩複雜誌（電子版）
중화손상여수복잡지（전자판）
Chinese Journal of Injury Repair and Wound Healing
2015年
2期
113-119
,共7页
马达%唐冰%石芬%曹小玲%朱家源
馬達%唐冰%石芬%曹小玲%硃傢源
마체%당빙%석분%조소령%주가원
免疫抑制剂%瘢痕%增生%芬戈莫德
免疫抑製劑%瘢痕%增生%芬戈莫德
면역억제제%반흔%증생%분과막덕
Immunosuppressive agents%Cicatrix%Hyperplasia%Fingolimod
目的：研究新型免疫抑制剂芬戈莫德对增生性瘢痕的抑制作用。方法40只新西兰兔（雌雄各半）于兔耳腹侧面瘢痕造模，每只兔每侧耳造模创面2个。按照完全随机的分组原则将所有兔分为5组，每组8只，每组32个瘢痕创面，按照溶剂、不同浓度芬戈莫德分别注射的处理方式分为：空白对照组、溶剂对照组、芬戈莫德2．5 mg／mL 组、芬戈莫德5．0 mg／mL 组、芬戈莫德10．0 mg／mL 组。瘢痕造模成功后一次性给药，观察并记录各组创面瘢痕大小变化，给药后第5天取材，对瘢痕组织进行组织学染色，量化并比较两组瘢痕创面的瘢痕表皮厚度、瘢痕胶原蛋白沉积情况及转化生长因子-β、结缔组织生长因子的蛋白表达情况。结果芬戈莫德各浓度组兔耳余量瘢痕面积比明显缩小；芬戈莫德2．5 mg／mL组瘢痕表皮厚度为（120．250±6．042）μm，瘢痕表皮增厚明显，胶原沉积率降低，分别与芬戈莫德5．0 mg／mL 组、芬戈莫德10．0 mg／mL 组比较，芬戈莫德2．5 mg／mL 组与瘢痕增生相关的转化生长因子-β、结缔组织生长因子含量下降，差异均有统计学意义（P ＜0．05）。结论新型免疫抑制剂芬戈莫德对瘢痕形成增生有抑制作用，芬戈莫德浓度为2．5 mg／mL 时效果最为显著。
目的：研究新型免疫抑製劑芬戈莫德對增生性瘢痕的抑製作用。方法40隻新西蘭兔（雌雄各半）于兔耳腹側麵瘢痕造模，每隻兔每側耳造模創麵2箇。按照完全隨機的分組原則將所有兔分為5組，每組8隻，每組32箇瘢痕創麵，按照溶劑、不同濃度芬戈莫德分彆註射的處理方式分為：空白對照組、溶劑對照組、芬戈莫德2．5 mg／mL 組、芬戈莫德5．0 mg／mL 組、芬戈莫德10．0 mg／mL 組。瘢痕造模成功後一次性給藥，觀察併記錄各組創麵瘢痕大小變化，給藥後第5天取材，對瘢痕組織進行組織學染色，量化併比較兩組瘢痕創麵的瘢痕錶皮厚度、瘢痕膠原蛋白沉積情況及轉化生長因子-β、結締組織生長因子的蛋白錶達情況。結果芬戈莫德各濃度組兔耳餘量瘢痕麵積比明顯縮小；芬戈莫德2．5 mg／mL組瘢痕錶皮厚度為（120．250±6．042）μm，瘢痕錶皮增厚明顯，膠原沉積率降低，分彆與芬戈莫德5．0 mg／mL 組、芬戈莫德10．0 mg／mL 組比較，芬戈莫德2．5 mg／mL 組與瘢痕增生相關的轉化生長因子-β、結締組織生長因子含量下降，差異均有統計學意義（P ＜0．05）。結論新型免疫抑製劑芬戈莫德對瘢痕形成增生有抑製作用，芬戈莫德濃度為2．5 mg／mL 時效果最為顯著。
목적：연구신형면역억제제분과막덕대증생성반흔적억제작용。방법40지신서란토（자웅각반）우토이복측면반흔조모，매지토매측이조모창면2개。안조완전수궤적분조원칙장소유토분위5조，매조8지，매조32개반흔창면，안조용제、불동농도분과막덕분별주사적처리방식분위：공백대조조、용제대조조、분과막덕2．5 mg／mL 조、분과막덕5．0 mg／mL 조、분과막덕10．0 mg／mL 조。반흔조모성공후일차성급약，관찰병기록각조창면반흔대소변화，급약후제5천취재，대반흔조직진행조직학염색，양화병비교량조반흔창면적반흔표피후도、반흔효원단백침적정황급전화생장인자-β、결체조직생장인자적단백표체정황。결과분과막덕각농도조토이여량반흔면적비명현축소；분과막덕2．5 mg／mL조반흔표피후도위（120．250±6．042）μm，반흔표피증후명현，효원침적솔강저，분별여분과막덕5．0 mg／mL 조、분과막덕10．0 mg／mL 조비교，분과막덕2．5 mg／mL 조여반흔증생상관적전화생장인자-β、결체조직생장인자함량하강，차이균유통계학의의（P ＜0．05）。결론신형면역억제제분과막덕대반흔형성증생유억제작용，분과막덕농도위2．5 mg／mL 시효과최위현저。
Objective To determine the therapeutic effect of fingolimod on hypertrophic scars. Methods Forty New zealand rabbits were used to construct hypertrophic scar models on the ears,and two masses of hypertrophic scars on each ear.The rabbits were divided into 5 groups by handling method:negative control group,solvent control group,fingolimod 2.5 mg/mL group,fingolimod 5.0 mg/mL group, fingolimod 1 0.0 mg/mL group,each group had 8 rabbits and 32 scare wound.The thickness and mass of hypertrophic scars were measured respectively,on the 5th day after injecting drug,the hypertrophic scars were harvested and detected by histological staining.The scar cuticle thickness,scar collagen deposition and protein expression of transforming growth factor beta and connective tissue growth factor were compared. Results The mass of hypertrophic scars in group fingolimod decreased significantly,the thickness of fingolimod 2.5 mg/mL group was (1 20.250 ±6.042)μm,collage deposition depressed.Compared to fingolimod 5.0 mg/mL,1 0.0 mg/mL group,the expression of transforming growth factor-βand connective tissue growth factor of fingolimod 2.5 mg/mL group descended,the difference was statistically significant (P <0.05).Conclusion Fingolimod can inhibit the hypertrophic scars,and 2.5 mg/mL fingolimod has the most significant effect.
