中华肝胆外科杂志
中華肝膽外科雜誌
중화간담외과잡지
CHINESE JOURNAL OF HEPATOBILIARY SURGERY
2015年
4期
253-256
,共4页
CCR4%趋化因子%胆囊癌%侵袭与转移
CCR4%趨化因子%膽囊癌%侵襲與轉移
CCR4%추화인자%담낭암%침습여전이
CCR4%Chemokine%Gallbladder cancer%Infiltration and metastasis
目的 研究胆囊癌组织中趋化因子受体CCR4的表达,以及CCR4与临床病理因素的相互关系及其对胆囊癌预后的影响.方法 免疫组织化学法检测CCR4在胆囊癌组织标本及慢性胆囊炎胆囊黏膜组织标本中的表达情况,结合临床资料进行分析.Kaplan-Meier法进行CCR4表达阳性组与阴性组生存曲线比较.Cox回归模型进行多因素分析.x2检验分析CCR4在胆囊癌组织中的表达与临床病理因素的相关性.结果 胆囊癌细胞胞浆和(或)胞膜有CCR4表达,呈棕黄色.胆囊癌CCR4蛋白的表达明显高于慢性胆囊炎胆囊上皮组织(P＜0.05).CCR4的高表达与胆囊癌患者的性别、年龄、病理分级、远处转移及神经淋巴管侵犯等因素无关,而与肿瘤淋巴结转移(P＜0.05)及组织学分级有关(P＜0.05).Kaplan Meier生存分析结果显示CCR4阳性组与阴性组术后生存时间差异有统计学意义(P＜0.05).多因素分析表明,CCR4表达水平是影响胆囊癌患者手术后生存的独立危险因素.结论 趋化因子受体CCR4在胆囊癌组织中有表达,且与肿瘤淋巴结转移及组织学分级有关,是胆囊癌患者术后生存的独立危险因素;趋化因子受体CCR4与胆囊癌的侵袭与转移有关.基于CCR4的抗肿瘤靶点技术有望成为治疗胆囊癌的一种新方式.
目的 研究膽囊癌組織中趨化因子受體CCR4的錶達,以及CCR4與臨床病理因素的相互關繫及其對膽囊癌預後的影響.方法 免疫組織化學法檢測CCR4在膽囊癌組織標本及慢性膽囊炎膽囊黏膜組織標本中的錶達情況,結閤臨床資料進行分析.Kaplan-Meier法進行CCR4錶達暘性組與陰性組生存麯線比較.Cox迴歸模型進行多因素分析.x2檢驗分析CCR4在膽囊癌組織中的錶達與臨床病理因素的相關性.結果 膽囊癌細胞胞漿和(或)胞膜有CCR4錶達,呈棕黃色.膽囊癌CCR4蛋白的錶達明顯高于慢性膽囊炎膽囊上皮組織(P＜0.05).CCR4的高錶達與膽囊癌患者的性彆、年齡、病理分級、遠處轉移及神經淋巴管侵犯等因素無關,而與腫瘤淋巴結轉移(P＜0.05)及組織學分級有關(P＜0.05).Kaplan Meier生存分析結果顯示CCR4暘性組與陰性組術後生存時間差異有統計學意義(P＜0.05).多因素分析錶明,CCR4錶達水平是影響膽囊癌患者手術後生存的獨立危險因素.結論 趨化因子受體CCR4在膽囊癌組織中有錶達,且與腫瘤淋巴結轉移及組織學分級有關,是膽囊癌患者術後生存的獨立危險因素;趨化因子受體CCR4與膽囊癌的侵襲與轉移有關.基于CCR4的抗腫瘤靶點技術有望成為治療膽囊癌的一種新方式.
목적 연구담낭암조직중추화인자수체CCR4적표체,이급CCR4여림상병리인소적상호관계급기대담낭암예후적영향.방법 면역조직화학법검측CCR4재담낭암조직표본급만성담낭염담낭점막조직표본중적표체정황,결합림상자료진행분석.Kaplan-Meier법진행CCR4표체양성조여음성조생존곡선비교.Cox회귀모형진행다인소분석.x2검험분석CCR4재담낭암조직중적표체여림상병리인소적상관성.결과 담낭암세포포장화(혹)포막유CCR4표체,정종황색.담낭암CCR4단백적표체명현고우만성담낭염담낭상피조직(P＜0.05).CCR4적고표체여담낭암환자적성별、년령、병리분급、원처전이급신경림파관침범등인소무관,이여종류림파결전이(P＜0.05)급조직학분급유관(P＜0.05).Kaplan Meier생존분석결과현시CCR4양성조여음성조술후생존시간차이유통계학의의(P＜0.05).다인소분석표명,CCR4표체수평시영향담낭암환자수술후생존적독립위험인소.결론 추화인자수체CCR4재담낭암조직중유표체,차여종류림파결전이급조직학분급유관,시담낭암환자술후생존적독립위험인소;추화인자수체CCR4여담낭암적침습여전이유관.기우CCR4적항종류파점기술유망성위치료담낭암적일충신방식.
Objective To study the expression of CCR4 in gallbladder carcinoma tissues,and to determine its relationship with clinical pathological factors and its influence on prognosis of gallbladder cancer.Methods The expressions of CCR4 in gallbladder carcinoma tissues,and chronic cholecystitis gallbladder mucosal tissues were detected using immune histochemical methods and they were analyzed together with the clinical records.Survival analysis was used to compare the expressions of CCR4 between the positive group and the negative group.Multiple factors analysis was carried out using the Cox regression model.Correlation between the expressions of CCR4 in gallbladder tissues and the clinical pathologic factors was done using the Chi-square test.Results CCR4 is expressed tan-yellow in gallbladder cell cytoplasm and/or cell membrane.The expression of CCR4 protein in the gallbladder carcinoma was obviously higher than in the chronic cholecystitis gallbladder epithelial tissues (P ＜ 0.05).A high expression of CCR4 was not correlated with patients' age,gender,pathological classification,distant metastasis and nerve/lymphatic invasion factors.It was,however correlated with tumor lymph node metastasis (P ＜ 0.05) and histological grading (P ＜ 0.05).Kaplan Meier survival analysis showed the postoperative survival between the CCR4 positive group and the negative group were significant different (P ＜0.05).On multiple factors analysis,CCR4 expression level was an independent risk factor of survival of patients after gallbladder surgery.Conclusions The chemokine receptor CCR4 expressed in gallbladder carcinoma.Its level of expression correlated with lymph node metastasis and histological grading.It was an independent risk factor of survival in patients with gallbladder carcinoma after surgery.As it was associated with invasion and metastasis of gallbladder carcinoma,antitumor treatment targeting CCR4 is expected to become a novel treatment of gallbladder carcinoma.
