CAJ | 학술논문

目的研究骨髓间充质干细胞(MSC)联合胰岛移植对滤泡辅助性T细胞(Tfh)分化的调节作用及诱导非肥胖糖尿病鼠(NOD)胰岛移植免疫耐受的影响.方法实验动物分为4组.A组:单纯胰岛移植组;B组:MSC与胰岛联合移植组(MSC:0.5 × 106个);C组:MSC与胰岛联合移植组(MSC:2×106个);D组:MSC与胰岛联合移植组(MSC:3×106个).ELISA法检测各组小鼠糖尿病自身抗体GAD65Ab、IAA表达水平,流式细胞仪检测各组外周血及脾脏Tfh细胞水平.结果与胰岛单独移植组NOD小鼠相比,MSC与胰岛联合移植组NOD小鼠胰岛移植物生存时间明显延长,体内糖尿病自身抗体GAD65 Ab和IAA浓度明显降低,CI4+T细胞向Tfh分化明显降低.结论 MSC联合胰岛移植可以通过调节Tfh细胞分化从而保护胰岛移植物.
목적 연구골수간충질간세포(MSC)연합이도이식대려포보조성T세포(Tfh)분화적조절작용급유도비비반당뇨병서(NOD)이도이식면역내수적영향.방법 실험동물분위4조.A조:단순이도이식조;B조:MSC여이도연합이식조(MSC:0.5 × 106개);C조:MSC여이도연합이식조(MSC:2×106개);D조:MSC여이도연합이식조(MSC:3×106개).ELISA법검측각조소서당뇨병자신항체GAD65Ab、IAA표체수평,류식세포의검측각조외주혈급비장Tfh세포수평.결과 여이도단독이식조NOD소서상비,MSC여이도연합이식조NOD소서이도이식물생존시간명현연장,체내당뇨병자신항체GAD65 Ab화IAA농도명현강저,CI4+T세포향Tfh분화명현강저.결론 MSC연합이도이식가이통과조절Tfh세포분화종이보호이도이식물.
Objective To study the regulation mechanism of bone mesenchymal stem cell (MSC)combined co-translation of islets in differentiation of Follicular Helper T cell (Tfh),and its roll on immunotolerence induction in non-obese diabetic (NOD) mice transplantation model.Methods The NOD mice were divided into 4 groups:Group A with islet transplantation alone;Group B with MSC co-transplantation with islets (MSC:0.5 × 106);Group C with MSC co-transplantation with islets (MSC:2 × 106);Group D with MSC co-transplantation with islets (MSC:3 × 106).ELISA was used to test the expression level of diabetes autoantibody GAD65Ab and IAA.Tfh cell count was detected by FACS.Results The survival time of transplantation groups was much longer in MSCs co-transplantation group than islet-alone group;the level of GAD65Ab,IAA and Tfh cell count were much lower in MSCs co-transplantation group than islet-alone group.Conclusion MSC may protect the islet transplants by regulating the Tfh cell differentiation.