中华预防医学杂志
中華預防醫學雜誌
중화예방의학잡지
CHINESE JOURNAL OF
2015年
3期
248-253
,共6页
肥胖症%脂肪组织%卷曲螺旋结构域80(CCDC80)%胰岛素抵抗
肥胖癥%脂肪組織%捲麯螺鏇結構域80(CCDC80)%胰島素牴抗
비반증%지방조직%권곡라선결구역80(CCDC80)%이도소저항
Obesity%Adipose tissue%Coiled-coil domain-containing protein 80 (CCDC80)%Insulin resistance (IR)
目的 探究人体血清和脂肪组织中脂肪因子卷曲螺旋结构域80(CCDC80)表达水平与肥胖之间的关系.方法 于2010年9月,采用横断面调查方法在唐山某医院招募100名社区人群(包括健康人群78名和2型糖尿病患者22例)和该院36例妇产科良性肿瘤患者,调查其人口学基本特征并收集血清,检测血液生化指标.社区人群和妇产科良性肿瘤患者均按照BMI分为正常体重组(BMI＜ 24.0 kg/m2)和超重肥胖组(BMI≥24.0 kg/m2).对100名社区人群以ELISA检测血清CCDC80水平.对36例妇产科良性肿瘤患者采集并保存内脏和皮下脂肪组织,以Real-time PCR检测脂肪中CCDC80 mRNA表达水平.采用Pearson简单相关及多元线性回归方法,分别分析血清和脂肪组织中CCDC80表达水平与腰围、BMI和其他生化指标之间的相关性.结果 100名社区人群年龄为(52.9±8.4)岁,男、女分别为44、56名.按照不同体重分组后,调整年龄、性别后进行协方差分析,各组HDL-C水平为:超重肥胖合并糖尿病组为(0.90±0.06) mmol/L,单纯超重肥胖组为(1.14±0.04) mmol/L,正常体重组为(1.28±0.05) mmol/L,差异有统计学意义(F=10.73,P＜O.001).调整年龄、性别后的血清CCDC80对数值的面±s为(5.84±0.16) pg/ml,男性为(5.81 ±0.98) pg/ml,女性为(5.97±0.89) pg/ml,性别间差异无统计学意义(t=-0.81,P=0.419).方差分析结果显示,正常体重组血清CCDC80水平为(5.84±0.16) pg/ml,单纯超重肥胖组为(6.11±0.14) pg/ml,超重肥胖合并糖尿病组为(5.84±0.19) pg/ml,不同体重组人群血清CCDC80水平之间差异无统计学意义(F=1.06,P=0.351).血清CCDC80水平与FBG呈负相关(Rh=-0.223,P=0.026);多元线性回归模型显示FBG浓度每上升1 mmol/L,血清CCDC80浓度下降0.24 pg/ml(β=-0.24,95％ CI:-0.21 ～-0.02).进一步发现单纯超重肥胖组,血清CCDC80水平与FBG也呈负相关(Ra=-0.368,P=0.013).此外,CCDC80在36例皮下和内脏脂肪组织中均有表达,皮下脂肪相对表达水平为0.06 ±0.02,内脏脂肪为0.05 ±0.04.且超重肥胖者内脏脂肪CCDC80 mRNA相对表达水平为0.05±0.03,低于皮下脂肪中的相对表达水平0.06 ±0.03(t =2.50,P=0.025).进一步在超重肥胖人群中的Pearson相关分析发现,腰围与内脏脂肪CCDC80基因表达水平呈负相关(r=-0.472,P=0.035).结论 脂肪因子CCDC80可能参与体内能量及胰岛素代谢,是肥胖和胰岛素抵抗的保护因素之一.
目的 探究人體血清和脂肪組織中脂肪因子捲麯螺鏇結構域80(CCDC80)錶達水平與肥胖之間的關繫.方法 于2010年9月,採用橫斷麵調查方法在唐山某醫院招募100名社區人群(包括健康人群78名和2型糖尿病患者22例)和該院36例婦產科良性腫瘤患者,調查其人口學基本特徵併收集血清,檢測血液生化指標.社區人群和婦產科良性腫瘤患者均按照BMI分為正常體重組(BMI＜ 24.0 kg/m2)和超重肥胖組(BMI≥24.0 kg/m2).對100名社區人群以ELISA檢測血清CCDC80水平.對36例婦產科良性腫瘤患者採集併保存內髒和皮下脂肪組織,以Real-time PCR檢測脂肪中CCDC80 mRNA錶達水平.採用Pearson簡單相關及多元線性迴歸方法,分彆分析血清和脂肪組織中CCDC80錶達水平與腰圍、BMI和其他生化指標之間的相關性.結果 100名社區人群年齡為(52.9±8.4)歲,男、女分彆為44、56名.按照不同體重分組後,調整年齡、性彆後進行協方差分析,各組HDL-C水平為:超重肥胖閤併糖尿病組為(0.90±0.06) mmol/L,單純超重肥胖組為(1.14±0.04) mmol/L,正常體重組為(1.28±0.05) mmol/L,差異有統計學意義(F=10.73,P＜O.001).調整年齡、性彆後的血清CCDC80對數值的麵±s為(5.84±0.16) pg/ml,男性為(5.81 ±0.98) pg/ml,女性為(5.97±0.89) pg/ml,性彆間差異無統計學意義(t=-0.81,P=0.419).方差分析結果顯示,正常體重組血清CCDC80水平為(5.84±0.16) pg/ml,單純超重肥胖組為(6.11±0.14) pg/ml,超重肥胖閤併糖尿病組為(5.84±0.19) pg/ml,不同體重組人群血清CCDC80水平之間差異無統計學意義(F=1.06,P=0.351).血清CCDC80水平與FBG呈負相關(Rh=-0.223,P=0.026);多元線性迴歸模型顯示FBG濃度每上升1 mmol/L,血清CCDC80濃度下降0.24 pg/ml(β=-0.24,95％ CI:-0.21 ～-0.02).進一步髮現單純超重肥胖組,血清CCDC80水平與FBG也呈負相關(Ra=-0.368,P=0.013).此外,CCDC80在36例皮下和內髒脂肪組織中均有錶達,皮下脂肪相對錶達水平為0.06 ±0.02,內髒脂肪為0.05 ±0.04.且超重肥胖者內髒脂肪CCDC80 mRNA相對錶達水平為0.05±0.03,低于皮下脂肪中的相對錶達水平0.06 ±0.03(t =2.50,P=0.025).進一步在超重肥胖人群中的Pearson相關分析髮現,腰圍與內髒脂肪CCDC80基因錶達水平呈負相關(r=-0.472,P=0.035).結論 脂肪因子CCDC80可能參與體內能量及胰島素代謝,是肥胖和胰島素牴抗的保護因素之一.
목적 탐구인체혈청화지방조직중지방인자권곡라선결구역80(CCDC80)표체수평여비반지간적관계.방법 우2010년9월,채용횡단면조사방법재당산모의원초모100명사구인군(포괄건강인군78명화2형당뇨병환자22례)화해원36례부산과량성종류환자,조사기인구학기본특정병수집혈청,검측혈액생화지표.사구인군화부산과량성종류환자균안조BMI분위정상체중조(BMI＜ 24.0 kg/m2)화초중비반조(BMI≥24.0 kg/m2).대100명사구인군이ELISA검측혈청CCDC80수평.대36례부산과량성종류환자채집병보존내장화피하지방조직,이Real-time PCR검측지방중CCDC80 mRNA표체수평.채용Pearson간단상관급다원선성회귀방법,분별분석혈청화지방조직중CCDC80표체수평여요위、BMI화기타생화지표지간적상관성.결과 100명사구인군년령위(52.9±8.4)세,남、녀분별위44、56명.안조불동체중분조후,조정년령、성별후진행협방차분석,각조HDL-C수평위:초중비반합병당뇨병조위(0.90±0.06) mmol/L,단순초중비반조위(1.14±0.04) mmol/L,정상체중조위(1.28±0.05) mmol/L,차이유통계학의의(F=10.73,P＜O.001).조정년령、성별후적혈청CCDC80대수치적면±s위(5.84±0.16) pg/ml,남성위(5.81 ±0.98) pg/ml,녀성위(5.97±0.89) pg/ml,성별간차이무통계학의의(t=-0.81,P=0.419).방차분석결과현시,정상체중조혈청CCDC80수평위(5.84±0.16) pg/ml,단순초중비반조위(6.11±0.14) pg/ml,초중비반합병당뇨병조위(5.84±0.19) pg/ml,불동체중조인군혈청CCDC80수평지간차이무통계학의의(F=1.06,P=0.351).혈청CCDC80수평여FBG정부상관(Rh=-0.223,P=0.026);다원선성회귀모형현시FBG농도매상승1 mmol/L,혈청CCDC80농도하강0.24 pg/ml(β=-0.24,95％ CI:-0.21 ～-0.02).진일보발현단순초중비반조,혈청CCDC80수평여FBG야정부상관(Ra=-0.368,P=0.013).차외,CCDC80재36례피하화내장지방조직중균유표체,피하지방상대표체수평위0.06 ±0.02,내장지방위0.05 ±0.04.차초중비반자내장지방CCDC80 mRNA상대표체수평위0.05±0.03,저우피하지방중적상대표체수평0.06 ±0.03(t =2.50,P=0.025).진일보재초중비반인군중적Pearson상관분석발현,요위여내장지방CCDC80기인표체수평정부상관(r=-0.472,P=0.035).결론 지방인자CCDC80가능삼여체내능량급이도소대사,시비반화이도소저항적보호인소지일.
Objective To explore the relationship between expression of coiled-coil domaincontaining protein 80 (CCDC80) and obesity in serum and adipose tissues.Methods A cross-sectional survey was conducted in a hospital in Tangshan in September 2010.100 people including 78 healthy people and 22 with type-2 diabetes were recruited.Another 36 female patients with benign tumor of Obstetrics and Gynecology were also recruited.Demographic characteristics and serum samples were collected from all subjects,basic biochemical indicators were tested.All subjects were divided into ‘Normal Weight’ and ‘Overweight and Obese’ according to their BMI (BMI ＜24.0 kg/m2;BMI≥24 kg/m2).Serum CCDC80 of the 100 subjects was detected by ELISA.Visceral and subcutaneous fat were derived from the other 36 female subjects,and Real-time PCR was used to detect CCDC80 mRNA expression in adipose tissues.Pearson correlation and multiple linear regression were used to analyze the correlation between serum or adipose CCDC80 expression and waist,BMI,and other biochemical indicators.Results The age of 100 subjects was (52.9 ± 8.4) years old.44％ of them were women (44 cases) and 56％ of them were men (56 cases).After dividing them into three groups according to their BMI,covariance analysis were conducted,and age and gender were adjusted.The HDL-C level was significantly different among three groups (F =10.73,P ＜ 0.001):‘ Overweight and obese combined with diabetes’ group ((0.90 ± 0.06) mmol/L) ＜ ‘Overweight and obese’ group ((1.14 ± 0.04) mmol/L) ＜ ‘ Normal weight’ group ((1.28 ± 0.05)mmol/L).The adjusted expression of serum CCDC80 of the 100 subjects was (5.84 ± 0.16) pg/ml,(5.81 ±0.98) pg/ml among men and (5.97 ±0.89)pg/ml among women,and there was no significant difference(t =-0.812,P=0.419) between genders.ANOVA revealed that there was no significant differences of the expression of serum CCDC80 among three groups (F =1.06,P =0.351),‘ Normal weight’ group was (5.84±0.16) pg/ml,‘overweight and obese’ group was (6.11 ±0.14) pg/ml,and ‘Overweight and obese combined with diabetes’ group was (5.84 ±0.19) pg/ml.The analysis showed that FBG had a negative correlation with serum CCDC80 (Rb =-0.223,P =0.026).Multivariate linear regression had a similar result,with 1 mmol/L increase of serum FBG,serum CCDC80 decreases for 0.24 pg/ml (3 =-0.24,95％ CI:-0.21--0.02).There was also a negative correlation between serum CCDC80 and FBG in overweight and obese people(Ra =-0.368,P =0.013).Besides,CCDC80 mRNA was detected in both subcutaneous and visceral adipose tissue of 36 cases,the expression level was 0.06 ± 0.02 for subcutaneous fat,was 0.05 ± 0.04 for visceral fat,and the expression in visceral fat was lower (0.05 ± 0.03) than that in subcutaneous fat (0.06 ± 0.03) (t =2.50,P =0.025) among overweight and obese group.There was a negative correlation between waist and visceral CCDC80 mRNA expression (r =-0.472,P =0.035).Conclusion This study suggested that CCDC80 may be involved in energy and insulin metabolism,and plays a protective role in obesity and diabetes.
