CAJ | 학술논문

目的探讨高脂膳食诱导的肥胖小鼠十二指肠组织中介导Fe2摄取和释放的关键分子——二价金属离子转运蛋白(DMT1)和膜铁转运蛋白(Fpn1)的基因表达变化.方法 C57BL/6J小鼠依随机数字表法分为正常对照(NC)组和肥胖模型(OM)组,每组6只,分别以普通饲料和高脂饲料喂养14周.肥胖建模成功后,采用Real-time PCR和Western blot法检测小鼠十二指肠DMT1、Fpn1mRNA和蛋白表达水平.结果 Real-time PCR检测结果显示,与NC组相比(将NC组mRNA表达水平定为1.00),OM组小鼠Fpn1 mRNA的相对表达水平(0.58±0.11)明显降低,差异有统计学意义(=6.71,P=0.014),而DMT1 mRNA相对表达水平(0.89 ±0.26)差异无统计学意义(t=2.01,P=0.122).Western blot检测结果显示,OM组小鼠十二指肠Fpn1蛋白表达量低于NC组,OM组和NC组相对表达水平分别为0.32±0.06、0.65 ±0.19,差异有统计学意义(t=5.37,P=0.026),而DMT1蛋白相对表达水平在OM组和NC组分别为0.88 ±0.21、0.92 ±0.17,差异无统计学意义(t=1.84,P=0.185).结论肥胖小鼠十二指肠Fpn1 mRNA及蛋白表达水平下降,而DMT1表达水平无明显变化,提示肥胖对肠铁吸收的影响可能在于肠黏膜细胞释放Fe2入血循环障碍.
목적 탐토고지선식유도적비반소서십이지장조직중개도Fe2섭취화석방적관건분자——이개금속리자전운단백(DMT1)화막철전운단백(Fpn1)적기인표체변화.방법 C57BL/6J소서의수궤수자표법분위정상대조(NC)조화비반모형(OM)조,매조6지,분별이보통사료화고지사료위양14주.비반건모성공후,채용Real-time PCR화Western blot법검측소서십이지장DMT1、Fpn1mRNA화단백표체수평.결과 Real-time PCR검측결과현시,여NC조상비(장NC조mRNA표체수평정위1.00),OM조소서Fpn1 mRNA적상대표체수평(0.58±0.11)명현강저,차이유통계학의의(=6.71,P=0.014),이DMT1 mRNA상대표체수평(0.89 ±0.26)차이무통계학의의(t=2.01,P=0.122).Western blot검측결과현시,OM조소서십이지장Fpn1단백표체량저우NC조,OM조화NC조상대표체수평분별위0.32±0.06、0.65 ±0.19,차이유통계학의의(t=5.37,P=0.026),이DMT1단백상대표체수평재OM조화NC조분별위0.88 ±0.21、0.92 ±0.17,차이무통계학의의(t=1.84,P=0.185).결론 비반소서십이지장Fpn1 mRNA급단백표체수평하강,이DMT1표체수평무명현변화,제시비반대장철흡수적영향가능재우장점막세포석방Fe2입혈순배장애.
Objective This study aims to determine the gene expression changes of iron transporters-divalent metal transporter 1 (DMT1) and ferroportin 1 (Fpn1) in the duodenal tissue of diet-induced obese mice.Methods C57BL/6J mice were randomly divided into normal control(NC) and obesity model(OM) group,6 in each,and fed on conventional and high-fat diet respectively for 14 weeks by table of random number.Then the DMT1 and Fpn1 mRNA contents in duodenal tissues of the animals were measured by Real-time PCR method,and the protein expression levels were analyzed by Western blot test.Results The Real-time PCR detection results showed that,compared with the NC group for which the mRNA expression level was defined as 1.0,the Fpn1 mRNA expression in OM group (0.58 ± 0.11) was reduced significantly(t =6.71,P =0.014),whereas the relative expression level of DMT1 mRNA in OM group(0.89±0.26) showed no obvious alteration(t =2.01,P =0.122).Western blot results showed that the relative protein expression levels of Fpn1 in OM and NC group were 0.32 ± 0.06 and 0.65 ± 0.19,respectively,and the difference was statistically significant (t =5.37,P =0.026).The DMT1 protein relative abundance was 0.88 ±0.21 in OM group and 0.92 ±0.17 in NC group,and the difference has no statistical significance (t =1.84,P =0.185).Conclusion Fpnl gene expression is inhibited in the duodenum of diet-induced obesity mouse while DMT1 expression keeps unchanged,and this implies that decreased iron export from enterocytes into circulation might be responsible for the impaired iron absorption in obesity.