CAJ | 학술논문

脂质转运蛋白(lipid transfer protein, LTP)是广泛存在于植物中的一类小分子蛋白,因其能在植物细胞膜间运输脂类物质而得名,在植物角质层合成和适应多种环境胁迫中起重要作用。本研究利用同源克隆方法得到 TaLTP-s的cDNA全长序列510 bp,开放阅读框为339 bp,编码112个氨基酸。利用RIL群体将TaLTP-s定位在染色体1A上,距离两侧标记WMC449和WMC93的遗传距离分别为2.1 cM和5.9 cM。通过原生质体亚细胞定位显示TaLTP-s定位于细胞膜和细胞质中。小麦开花期 TaLTP-s 在小花中表达量较高,尤其是在花药中的表达量远高于在根和叶中。在ABA、PEG、NaCl及4℃低温胁迫诱导下,小麦TaLTP-s均上调表达,可能与抗逆性调节相关。在高盐胁迫处理下,转基因拟南芥幼苗的细胞膜稳定性和存活率显著高于野生型。研究结果为利用小麦脂质转运蛋白基因改良作物抗逆性奠定了基础。
지질전운단백(lipid transfer protein, LTP)시엄범존재우식물중적일류소분자단백,인기능재식물세포막간운수지류물질이득명,재식물각질층합성화괄응다충배경협박중기중요작용。본연구이용동원극륭방법득도 TaLTP-s적cDNA전장서렬510 bp,개방열독광위339 bp,편마112개안기산。이용RIL군체장TaLTP-s정위재염색체1A상,거리량측표기WMC449화WMC93적유전거리분별위2.1 cM화5.9 cM。통과원생질체아세포정위현시TaLTP-s정위우세포막화세포질중。소맥개화기 TaLTP-s 재소화중표체량교고,우기시재화약중적표체량원고우재근화협중。재ABA、PEG、NaCl급4℃저온협박유도하,소맥TaLTP-s균상조표체,가능여항역성조절상관。재고염협박처리하,전기인의남개유묘적세포막은정성화존활솔현저고우야생형。연구결과위이용소맥지질전운단백기인개량작물항역성전정료기출。
Lipid transfer protein (LTP) is a kind of small molecular protein, which name stands for its ability to transfer lipid be-tween cell membranes in plant. LTP plays a key role in cuticle synthesis and adaptation to abiotic stress. We cloned a full-length cDNA sequence of TaLTP gene encoding a lipid transfer protein from wheat (Triticum aestivum L.), which length is 510 bp with a 339 bp open reading frame. The cDNA sequence contains 112 amino acids with a N-terminal signal peptide within the first 25 amino acids. This gene contains eight cysteine residues conserved in amino acid sequences. TaLTP-s was detected to be located between markers WMC449 and WMC93 on chromosome 1A in wheat, with genetic distances of 2.1 cM and 5.9 cM, respectively. The result of subcellular localization exhibited that TaLTP-s is located in the cell membrane and cytoplasm. TaLTP-s was ex-pressed in all tissues at flowering stage, including leaf, root, floret, anther and pistil, and mature seeds of wheat. The highest ex-pression level was identified in the floret, especially in the anther. TaLTP-s was up-regulated by ABA, PEG, NaCl, and 4oC treat-ments which indicates that TaLTP-s is involved in different signal pathways responding to abiotic stress. Arabidopsis thaliana overexpressing TaLTP-s showed higher cell membrane stability and survival rate than the controls under salinity stress. These results provide a candidate gene for wheat improvement in response to salt and other abiotic stresses.