中国医药
中國醫藥
중국의약
CHINA MEDICINE
2015年
5期
742-745
,共4页
朱扶蓉%徐驯宇%林玲%黄郴%庄彬彬
硃扶蓉%徐馴宇%林玲%黃郴%莊彬彬
주부용%서순우%림령%황침%장빈빈
人食管鳞癌细胞%免疫磁珠分选%紫杉醇%耐药
人食管鱗癌細胞%免疫磁珠分選%紫杉醇%耐藥
인식관린암세포%면역자주분선%자삼순%내약
Human esophageal squamous cell carcinoma%Magnetic activated cell sorting%Paclitaxel%Resistance
目的 探讨神经生长因子受体阳性(NGFR+)人食管鳞癌细胞株EC9706对紫杉醇的耐药性.方法 采用噻唑蓝法药物敏感试验测定NGFR+ EC9706细胞的耐药指数,采用小鼠致瘤实验模型评估紫杉醇作用下的NGFR+ EC9706细胞与未分选EC9706细胞异种移植肿瘤生长的差异.结果 NGFR+EC9706细胞的耐药指数为2.03,NGFR+ EC9706细胞致瘤的紫杉醇组与0.9％氯化钠溶液组瘤重无明显差异(P＞0.05),实体瘤大小差别不明显.而未分选EC9706细胞致瘤的紫杉醇组与0.9％氯化钠溶液组比较瘤重明显减小[(0.5±0.4)g比(1.9±1.0)g],差异有统计学意义(P＜0.05),实体瘤也明显减小.未分选EC9706细胞致瘤的紫杉醇组较NGFR+ EC9706细胞致瘤的紫杉醇组瘤重明显减小[(0.5±0.4)g比(1.3±0.4)g],差异有统计学意义(P＜0.05).结论 人食管鳞癌细胞中的NGFR+ EC9706细胞对紫杉醇具有一定的耐药性.
目的 探討神經生長因子受體暘性(NGFR+)人食管鱗癌細胞株EC9706對紫杉醇的耐藥性.方法 採用噻唑藍法藥物敏感試驗測定NGFR+ EC9706細胞的耐藥指數,採用小鼠緻瘤實驗模型評估紫杉醇作用下的NGFR+ EC9706細胞與未分選EC9706細胞異種移植腫瘤生長的差異.結果 NGFR+EC9706細胞的耐藥指數為2.03,NGFR+ EC9706細胞緻瘤的紫杉醇組與0.9％氯化鈉溶液組瘤重無明顯差異(P＞0.05),實體瘤大小差彆不明顯.而未分選EC9706細胞緻瘤的紫杉醇組與0.9％氯化鈉溶液組比較瘤重明顯減小[(0.5±0.4)g比(1.9±1.0)g],差異有統計學意義(P＜0.05),實體瘤也明顯減小.未分選EC9706細胞緻瘤的紫杉醇組較NGFR+ EC9706細胞緻瘤的紫杉醇組瘤重明顯減小[(0.5±0.4)g比(1.3±0.4)g],差異有統計學意義(P＜0.05).結論 人食管鱗癌細胞中的NGFR+ EC9706細胞對紫杉醇具有一定的耐藥性.
목적 탐토신경생장인자수체양성(NGFR+)인식관린암세포주EC9706대자삼순적내약성.방법 채용새서람법약물민감시험측정NGFR+ EC9706세포적내약지수,채용소서치류실험모형평고자삼순작용하적NGFR+ EC9706세포여미분선EC9706세포이충이식종류생장적차이.결과 NGFR+EC9706세포적내약지수위2.03,NGFR+ EC9706세포치류적자삼순조여0.9％록화납용액조류중무명현차이(P＞0.05),실체류대소차별불명현.이미분선EC9706세포치류적자삼순조여0.9％록화납용액조비교류중명현감소[(0.5±0.4)g비(1.9±1.0)g],차이유통계학의의(P＜0.05),실체류야명현감소.미분선EC9706세포치류적자삼순조교NGFR+ EC9706세포치류적자삼순조류중명현감소[(0.5±0.4)g비(1.3±0.4)g],차이유통계학의의(P＜0.05).결론 인식관린암세포중적NGFR+ EC9706세포대자삼순구유일정적내약성.
Objective To explore the resistance of human esophageal squamous carcinoma cell line EC9706 with positive nerve growth factor receptor (NGFR) expression to paclitaxel.Methods Resistance index of NGFR + EC9706 was measured by four methyl tetrazolium methods in vitro.The growth of NGFR + EC9706 and non-sorted EC9706 was assessed by transplantation tumor model in nude mice.Results The resistance index of NGFR + EC9706 was 2.03.In transplantation tumor model induced by NGFR + EC9706,the tumor weight showed no significant difference between paclitaxel group and saline group;in transplantation tumor model induced by nonsorted EC9706,the tumor weight was significantly reduced in paclitaxel group compared with that in saline group [(0.5 ± 0.4) g vs (1.9 ± 1.0) g,P ＜ 0.05].In addition,the tumor weigh was slighter in non-sorted EC9706 induced transplantation tumor than that in NGFR + EC9706 induced transplantation tumor [(0.5 ± 0.4) g vs (1.3 ± 0.4) g,P ＜ 0.05].Conclusion NGFR + EC9706 cells are resistant to paclitaxel.
