CAJ | 학술논문

目的：研究光引发聚合改性明胶水凝胶的表征，理化性质以及生物相容性，为牙髓再生引入一种可能的新型复合支架材料。方法甲基丙烯酸将明胶改性后形成甲基丙烯明胶，用核磁共振技术检测其表征，用扫描电镜观察表面形貌，甲基丙烯明胶溶液加入艳固佳2959型光引发剂，经紫外光辐照后形成水凝胶，用流变仪测量黏弹性来反映力学性能，用台盼蓝染色法和CCK-8法检测水凝胶对人牙髓细胞增殖的影响。结果甲基丙烯酸改性明胶增加了明胶机械性能和孔隙率的同时，能够支持人牙髓细胞生长。结论甲基丙烯明胶水凝胶有望成为人牙髓再生研究中一种有潜力的支架材料。
목적：연구광인발취합개성명효수응효적표정，이화성질이급생물상용성，위아수재생인입일충가능적신형복합지가재료。방법갑기병희산장명효개성후형성갑기병희명효，용핵자공진기술검측기표정，용소묘전경관찰표면형모，갑기병희명효용액가입염고가2959형광인발제，경자외광복조후형성수응효，용류변의측량점탄성래반영역학성능，용태반람염색법화CCK-8법검측수응효대인아수세포증식적영향。결과갑기병희산개성명효증가료명효궤계성능화공극솔적동시，능구지지인아수세포생장。결론갑기병희명효수응효유망성위인아수재생연구중일충유잠력적지가재료。
Objective??The?objective?of?this?study?is?to?determine?the?characteristics,?physicochemical?property,?and?bio-compatibility?of?gelatin?methacrylate(GM)?hydrogel,?with?an?aim?to?introduce?this?as?a?new?scaffold?compound?for?dental?pulp?regeneration.?Methods Gelatin(G)?was?cross-linked?with?methylacrylic?acid(MA)?to?produce?GM.?Its?surface?appearance?was?assessed?using?scanning?electron?microscope(SEM),?and?its?characteristics?were?tested?using?nuclear?magnetic?resonance(NMR).?Hydrogel?is?formed?by?adding?Irgacure2959?into?the?GM?solution.?The?mixture?was?subsequently?exposedto?ultraviolet?ray(UV).?Rheometer?was?used?to?test?viscoelastity,?which?reflects?the?mechanical?property?of?GM.?The?effects?of?GM?to?the?proliferation?of?human?dental?pulp?cells(hDPCs)?were?revealed?by?Trypan?Blue?and?cell?counting?kit-8(CCK-8).?Results???GM?hydrogel,?which?was?formed?from?cross-linking?of?gelatin?and?methylacrylic?acid,?not?only?have?been?improved?in?its?mechanical?property?and?porosity,?but?also?supported?the?growth?of?hDPCs.?Conclusion???GM?hydrogel?is?a?potential?scaffold?material?in?dental?pulp?regeneration?research.