CAJ | 학술논문

目的：为探索胃癌分子成像技术的新方法，构建一套新型灵活的宽视场荧光内镜系统，并用于裸鼠胃癌移植瘤模型荧光成像研究。方法通过合理的光路设计及光学组件机加工，装配宽视场荧光内镜系统，通过对不同浓度的2－DG－750荧光探针进行成像，分析系统性能；对尾静脉注射100μl 2－DG－750荧光探针的裸鼠胃癌移植瘤模型进行在体荧光成像，对ROI的像素值和成像图进行分析。结果体外成像ROI的像素值与荧光探针浓度存在较好的线性回归关系，在2－DG－750剂量为31．3 pmol时，该系统即可检测到荧光信号，提示该系统灵敏性好；在体成像显示用该系统联合2－DG－750荧光探针观察到了肿瘤组织的较高荧光信号强度。结论宽视场荧光内镜系统具有很好的灵敏性，可以同时实现白光及荧光的双模式成像，具有实现光学分子成像诊断胃癌的潜在应用价值。
목적：위탐색위암분자성상기술적신방법，구건일투신형령활적관시장형광내경계통，병용우라서위암이식류모형형광성상연구。방법통과합리적광로설계급광학조건궤가공，장배관시장형광내경계통，통과대불동농도적2－DG－750형광탐침진행성상，분석계통성능；대미정맥주사100μl 2－DG－750형광탐침적라서위암이식류모형진행재체형광성상，대ROI적상소치화성상도진행분석。결과체외성상ROI적상소치여형광탐침농도존재교호적선성회귀관계，재2－DG－750제량위31．3 pmol시，해계통즉가검측도형광신호，제시해계통령민성호；재체성상현시용해계통연합2－DG－750형광탐침관찰도료종류조직적교고형광신호강도。결론관시장형광내경계통구유흔호적령민성，가이동시실현백광급형광적쌍모식성상，구유실현광학분자성상진단위암적잠재응용개치。
Objective To setup a new flexible wide-field epi-fluorescence endoscope system, then make a fluorescence ima-ging for gastric cancer xenograft model in nude mice by this system, so as to setup a new device and method for the diagnosis of gastric cancer molecular imaging techniques.Methods The widefield fluorescence endoscope system was assembledusing the rational optical path and optical components machining.Different concentrations of 2-DG-750 fluorescent probe was used to analyze the performance of the imaging system.By intravenous injection of 100μl2-DG-750 fluorescent probe into the xenografted tumor model,in vivo fluorescence imaging wasconducted, and ROI pixel values and imaging map were analyzed.Results The ROI pixel values demonstrated that a sen-sitivity of 31.3 pmoles 2-DG-750 was achieved as well as a good linear response from 1nmole to zero fluorescent probe.Fluorescence imaging indicated that gastrointestinal tumor tissues have an eminent 2-DG-750 uptake yielding a relatively strong fluorescence signal. Conclusions The new flexible wide-field epi-fluorescence endoscope system not only has a good sensitivity, but also can do a dual white and fluorescence imaging, which makes a promise future that optical technique will be applied to the diagnosis of gastric cancer.