河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2015年
4期
31-35
,共5页
张黛静%王多多%董文%马建辉%杨淑芳%张志娟%李春喜
張黛靜%王多多%董文%馬建輝%楊淑芳%張誌娟%李春喜
장대정%왕다다%동문%마건휘%양숙방%장지연%리춘희
小麦幼根%铜胁迫%转录组%蛋白组%差异表达
小麥幼根%銅脅迫%轉錄組%蛋白組%差異錶達
소맥유근%동협박%전록조%단백조%차이표체
wheat root%copper stress%transcriptomics%proteomics%differential expression
为探讨外源铜胁迫对小麦幼根的影响,采用水培方法,测定0~60 mg/ L 不同质量浓度铜处理下小麦幼苗生长发育、转录组表达和蛋白质组表达的差异,探索其抵御重金属毒害的分子机制。结果表明:高质量浓度的铜影响小麦生长,随胁迫时间的延长,小麦幼苗萎蔫发黄,幼根生长受抑。小麦幼根转录组测序检测,共得到2283个差异表达基因(DEGs),其中826个 DEGs 表达上调,1457个 DEGs表达下调;将 DEGs 进行 KEGG pathway 注释,被注释到31个代谢途径中。双向电泳试验检测,小麦幼根中有2049个蛋白质点,30 mg/ L 铜处理96 h 后,与对照(0 mg/ L 处理)相比,约130个蛋白质点表达出现显著差异,其中56个丰度增加,74个丰度降低;质谱鉴定部分差异表达蛋白,抗性蛋白如谷胱甘肽转移酶、27K 蛋白等在胁迫下表达上升,而生理代谢相关蛋白表达降低。
為探討外源銅脅迫對小麥幼根的影響,採用水培方法,測定0~60 mg/ L 不同質量濃度銅處理下小麥幼苗生長髮育、轉錄組錶達和蛋白質組錶達的差異,探索其牴禦重金屬毒害的分子機製。結果錶明:高質量濃度的銅影響小麥生長,隨脅迫時間的延長,小麥幼苗萎蔫髮黃,幼根生長受抑。小麥幼根轉錄組測序檢測,共得到2283箇差異錶達基因(DEGs),其中826箇 DEGs 錶達上調,1457箇 DEGs錶達下調;將 DEGs 進行 KEGG pathway 註釋,被註釋到31箇代謝途徑中。雙嚮電泳試驗檢測,小麥幼根中有2049箇蛋白質點,30 mg/ L 銅處理96 h 後,與對照(0 mg/ L 處理)相比,約130箇蛋白質點錶達齣現顯著差異,其中56箇豐度增加,74箇豐度降低;質譜鑒定部分差異錶達蛋白,抗性蛋白如穀胱甘肽轉移酶、27K 蛋白等在脅迫下錶達上升,而生理代謝相關蛋白錶達降低。
위탐토외원동협박대소맥유근적영향,채용수배방법,측정0~60 mg/ L 불동질량농도동처리하소맥유묘생장발육、전록조표체화단백질조표체적차이,탐색기저어중금속독해적분자궤제。결과표명:고질량농도적동영향소맥생장,수협박시간적연장,소맥유묘위언발황,유근생장수억。소맥유근전록조측서검측,공득도2283개차이표체기인(DEGs),기중826개 DEGs 표체상조,1457개 DEGs표체하조;장 DEGs 진행 KEGG pathway 주석,피주석도31개대사도경중。쌍향전영시험검측,소맥유근중유2049개단백질점,30 mg/ L 동처리96 h 후,여대조(0 mg/ L 처리)상비,약130개단백질점표체출현현저차이,기중56개봉도증가,74개봉도강저;질보감정부분차이표체단백,항성단백여곡광감태전이매、27K 단백등재협박하표체상승,이생리대사상관단백표체강저。
In order to explore the influence of exogenous copper(Cu) on roots of wheat,the solution cul-ture experiment was carried out to study the growth index,transcriptomics and proteomics differential ex-pression of wheat root. The results indicated that the growth of wheat was inhibited significantly,the influ-ence were closely related to Cu dose and time of treatment. The transcriptomics were analyzed,and 2 283 differential expression genes(DEGs) were detected,of which 826 DEGs up-regulated and 1 457 DEGs down-regulated significantly. All the DEGs were annotated by KEGG pathway to 31 metabolic pathways. About 2 049 differential expression proteins were detected by two-dimensional gel electrophoresis. After exposed to 30 mg / L Cu for 96 h,130 protein spots showed significant changes,among which 56 spots up-regulated and 74 spots down-regulated. The mass spectrometry analysis of part differential expression pro-teins showed that the resistant proteins were up-regulated in wheat roots,such as GSTs and 27K protein, but the expression of physiological metabolism related proteins down-regulated trend after being exposed to 30 mg / L Cu.