河南农业科学
河南農業科學
하남농업과학
JOURNAL OF HENAN AGRICULTURAL SCIENCES
2015年
4期
14-20
,共7页
陈晓杰%张建伟%杨保安%范家霖%张福彦%程仲杰%胡银岗
陳曉傑%張建偉%楊保安%範傢霖%張福彥%程仲傑%鬍銀崗
진효걸%장건위%양보안%범가림%장복언%정중걸%호은강
中国冬小麦%种质%遗传变异%遗传力%SSR 标记%遗传多样性
中國鼕小麥%種質%遺傳變異%遺傳力%SSR 標記%遺傳多樣性
중국동소맥%충질%유전변이%유전력%SSR 표기%유전다양성
Chinese winter wheat%germplasm%genetic variation%broad sense heritability%SSR marker%genetic diversity
为了明确中国冬小麦的遗传多样性,为育种工作提供有益信息,以90份具有广泛代表性的中国冬小麦核心种质为材料,通过连续2年度的水、旱处理,分析了其形态、光合生理及苗期根部共21个性状的遗传变异及广义遗传力;同时利用269对均匀分布于小麦21条染色体的 SSR 标记对供试材料进行遗传多样性分析。结果表明:除穗长、小穗数、净光合速率、胞间 CO2浓度和叶绿素含量5个性状外,其他16个性状均具有比较丰富的遗传变异;以形态性状为主的简单性状(穗叶距、穗下节长、株高、胚芽鞘长、根直径、千粒质量、穗长、叶绿素含量、穗粒数、小穗数、根长、旗叶长)具有较高的遗传力,适宜早代选择;产量性状(单株籽粒产量和单株生物学产量)遗传力中等,但考虑产量是育种改良的核心目标,也可作为早代选择指标;光合生理及复合性状(萌发期根表面积、根体积、胞间 CO2浓度、气孔导度、蒸腾速率和净光合速率)遗传力最低,尤其是光合生理性状易受外界环境的影响且不容易准确测量,不适宜作为早代选择的指标。 SSR 多样性分析表明,中国冬小麦遗传多样性较差;不同基因组间差异显著,其中 B 基因组多样性最丰富,D 基因组多样性最差。
為瞭明確中國鼕小麥的遺傳多樣性,為育種工作提供有益信息,以90份具有廣汎代錶性的中國鼕小麥覈心種質為材料,通過連續2年度的水、旱處理,分析瞭其形態、光閤生理及苗期根部共21箇性狀的遺傳變異及廣義遺傳力;同時利用269對均勻分佈于小麥21條染色體的 SSR 標記對供試材料進行遺傳多樣性分析。結果錶明:除穗長、小穗數、淨光閤速率、胞間 CO2濃度和葉綠素含量5箇性狀外,其他16箇性狀均具有比較豐富的遺傳變異;以形態性狀為主的簡單性狀(穗葉距、穗下節長、株高、胚芽鞘長、根直徑、韆粒質量、穗長、葉綠素含量、穗粒數、小穗數、根長、旂葉長)具有較高的遺傳力,適宜早代選擇;產量性狀(單株籽粒產量和單株生物學產量)遺傳力中等,但攷慮產量是育種改良的覈心目標,也可作為早代選擇指標;光閤生理及複閤性狀(萌髮期根錶麵積、根體積、胞間 CO2濃度、氣孔導度、蒸騰速率和淨光閤速率)遺傳力最低,尤其是光閤生理性狀易受外界環境的影響且不容易準確測量,不適宜作為早代選擇的指標。 SSR 多樣性分析錶明,中國鼕小麥遺傳多樣性較差;不同基因組間差異顯著,其中 B 基因組多樣性最豐富,D 基因組多樣性最差。
위료명학중국동소맥적유전다양성,위육충공작제공유익신식,이90빈구유엄범대표성적중국동소맥핵심충질위재료,통과련속2년도적수、한처리,분석료기형태、광합생리급묘기근부공21개성상적유전변이급엄의유전력;동시이용269대균균분포우소맥21조염색체적 SSR 표기대공시재료진행유전다양성분석。결과표명:제수장、소수수、정광합속솔、포간 CO2농도화협록소함량5개성상외,기타16개성상균구유비교봉부적유전변이;이형태성상위주적간단성상(수협거、수하절장、주고、배아초장、근직경、천립질량、수장、협록소함량、수립수、소수수、근장、기협장)구유교고적유전력,괄의조대선택;산량성상(단주자립산량화단주생물학산량)유전력중등,단고필산량시육충개량적핵심목표,야가작위조대선택지표;광합생리급복합성상(맹발기근표면적、근체적、포간 CO2농도、기공도도、증등속솔화정광합속솔)유전력최저,우기시광합생이성상역수외계배경적영향차불용역준학측량,불괄의작위조대선택적지표。 SSR 다양성분석표명,중국동소맥유전다양성교차;불동기인조간차이현저,기중 B 기인조다양성최봉부,D 기인조다양성최차。
In order to know the genetic diversity of Chinese winter wheat,and to provide useful informa-tion for wheat breeding,90 typical accessions of Chinese winter wheat were selected as research materials, which were planted in normal and drought conditions for two years. The genetic variations and broad sense heritability of 21 wheat traits were analyzed. The results showed that 16 traits had abundant genetic varia-tions(CVg > 10% ) except 5 traits( SL,SN,Pn,Ci and SPAD; CVg < 10% ); the simple traits mainly composed of morphological traits(SDL,UIL,PH,CL,RD,TKW,SL,SPAD,GNPS,SN,RL and FLL) had relatively higher heritability,which were available to be selected in early generations. The yield traits(BY-PP and GYPP) had medium heritability,which were considered to be reliable indexes in early generations as they were the core goal in wheat breeding. Photosynthetic and other complex traits(Pn,E,Gs,Ci,RS and RV) were of low heritability,especially the photosynthetic traits which were easily influenced by the environment and could not be evaluated accurately,thus they were unsuitable to be selected in early gen-erations. All the 90 wheat accessions were analyzed with 269 SSR markers distributing throughout the wheat genome. The result showed that the genetic diversity of the Chinese winter wheat accessions was at a lower level. Significant difference of genetic diversity was observed among the three genomes,of which the B genome showed the highest diversity and the D the lowest.