CAJ | 학술논문

目的：建立不同产地白英药材的HPLC指纹图谱，为建立白英药材的质量标准提供依据。方法：采用Waters C18（150 mm ×4.6 mm，5μm）（柱前加保护柱）色谱柱；流动相：乙腈-0.2%甲酸水溶液，梯度洗脱；流速：1.0 mL/min；检测波长：285 nm；柱温：室温。结果：通过对各个产地白英的HPLC指纹图谱进行直观分析，并采用中药色谱指纹图谱相似度软件进行相似度评价，经过比较，确定了13个峰作为特征峰，由此建立了白英的HPLC指纹图谱。结论：该法所得图谱清晰，特征直观，所建立的白英药材的HPLC指纹图谱可作为评价其质量的参考。
목적：건립불동산지백영약재적HPLC지문도보，위건립백영약재적질량표준제공의거。방법：채용Waters C18（150 mm ×4.6 mm，5μm）（주전가보호주）색보주；류동상：을정-0.2%갑산수용액，제도세탈；류속：1.0 mL/min；검측파장：285 nm；주온：실온。결과：통과대각개산지백영적HPLC지문도보진행직관분석，병채용중약색보지문도보상사도연건진행상사도평개，경과비교，학정료13개봉작위특정봉，유차건립료백영적HPLC지문도보。결론：해법소득도보청석，특정직관，소건립적백영약재적HPLC지문도보가작위평개기질량적삼고。
Objective:To establish HPLC finger prints of Solanum lyratum Thunb. of different areas and provide the basis for quality standard. Methods:Waters C18 (150 mm ×4.6 mm,5 μm) column was as chromatographic column,and mobile phase was acetonitrile-0.2% formic acid (gradient elution). Flow rate was 1.0 mL/min and detection wavelength was 285 nm. Column temperature was the room temperature. Results:HPLC finger prints of Solanum lyratum Thunb.of different ar-eas were analyzed and their similarities were evaluated through TCM fingerprint similarity evaluation softwar. 13 peaks in the spectrum were determined as characteristic peak of Solanum lyratum Thunb.. Conclusion:The spectrum is clear and could be used as the basis of quality evaluation.