CAJ | 학술논문

目的：探讨脾气虚型亚健康状态大鼠细胞免疫功能的改变。方法：多因素干预法（室温水游泳+夹尾+饮食限制等因素）制备脾气虚型亚健康状态大鼠模型，分为亚健康状态1、2、3组，分别给予1、2、3w的多因素干预，观察大鼠外观特征，计算脾脏指数与胸腺指数，采用免疫荧光流式细胞术检测T淋巴细胞及亚群的表达。结果：与正常组比较，脾气虚型亚健康状态各组大鼠脾脏指数与胸腺指数降低，CD+4、CD45RA+T 细胞表达率及CD45RA+/CD45RO+降低，CD4+CD45RO+、CD8+、CD8+CD45RO+、CD45RO+T细胞表达率升高，差异有统计学意义（P<0.05）。结论：T淋巴细胞亚群数目与比值的改变参与了脾气虚型亚健康状态大鼠的生理变化过程。
목적：탐토비기허형아건강상태대서세포면역공능적개변。방법：다인소간예법（실온수유영+협미+음식한제등인소）제비비기허형아건강상태대서모형，분위아건강상태1、2、3조，분별급여1、2、3w적다인소간예，관찰대서외관특정，계산비장지수여흉선지수，채용면역형광류식세포술검측T림파세포급아군적표체。결과：여정상조비교，비기허형아건강상태각조대서비장지수여흉선지수강저，CD+4、CD45RA+T 세포표체솔급CD45RA+/CD45RO+강저，CD4+CD45RO+、CD8+、CD8+CD45RO+、CD45RO+T세포표체솔승고，차이유통계학의의（P<0.05）。결론：T림파세포아군수목여비치적개변삼여료비기허형아건강상태대서적생리변화과정。
Objective:To investigate the change of cellular immune function of sub-health state rats with spleen qi deficien-cy. Methods:Rat models were prepared by the method of multi factors intervention(swimming in pool which water temper-ature was at room temperature combined dietary restriction and clampping tail). Rats were randomly divided into sub-health state 1、2、3 group which were respectively given multi factors intervention for 1、2、3 weeks ,then appearance char-acteristics of rats were observed,spleen index and thymus index were calculated,and expressions of T lymphocyte and its subsets were detected using immunofluorescence flow cytometry. Results:Compared with normal group,index of spleen and thymus of rats,expressions of CD+4、CD45RA+and CD45RA+/CD45RO+were decreased (P<0.05),expressions of CD+4CD45RO+、CD8+、CD8+CD45RO+、CD45RO+were increased(P<0.05) in sub-health state groups. Conclusion:The changes of number and ra-tio of T lymphocyte and its subsets could participate the process of physiological changes of sub-health state rats with spleen qi deficiency.