CAJ | 학술논문

目的：???测定抗菌肽P9-0对2种口腔感染常见细菌的抑菌活性，初探其抗菌机制。方法??选取口腔感染常见细菌金黄色葡萄球菌和具核梭杆菌，测定抗菌肽P9-0对2种细菌的最低抑菌浓度，通过扫描电子显微镜、荧光显微镜观其形貌。结果??抗菌肽P9-0对2种细菌最低抑菌浓度均为50μg·mL-1。扫描电子显微镜和荧光显微镜观察显示药物处理后，细菌胞膜在形态学和完整性上发生明显变化。结论???抗菌肽P9-0可抑制口腔感染常见之细菌金黄色葡萄球菌和具核梭杆菌，抗菌机制可能是通过胞膜破坏作用。
목적：???측정항균태P9-0대2충구강감염상견세균적억균활성，초탐기항균궤제。방법??선취구강감염상견세균금황색포도구균화구핵사간균，측정항균태P9-0대2충세균적최저억균농도，통과소묘전자현미경、형광현미경관기형모。결과??항균태P9-0대2충세균최저억균농도균위50μg·mL-1。소묘전자현미경화형광현미경관찰현시약물처리후，세균포막재형태학화완정성상발생명현변화。결론???항균태P9-0가억제구강감염상견지세균금황색포도구균화구핵사간균，항균궤제가능시통과포막파배작용。
Objective???This?study?aimed?to?determine?the?activity?of?antimicrobial?peptide?P9-0?on?pathogens?that?cause?oral?infection,?and?to?explore?preliminarily?the?mechanism?of?its?antibacterial?property.?Methods???Staphylococcus aureus(S. aureus)?and Fusobacterium nucleatum(F. nucleatum),which?are?common?pathogenic?bacteria?causing?oral?infection,?were?selected?as?the?research?organisms.?The?minimal?inhibitory?concentrations(MIC)?of?these?bacteria?were?measured?by?trace?dilution?method.?Changes?in?the?morphology?and?integrity?of?the?cell?membrane?of?these?bacteria?were?observed?under?fluorescence?microscope?and?scanning?electron?microscope.?Results???The?minimal?inhibitory?concentrations?of?P9-0?is?50 μg·mL-1?in?both?S. aureus?and?F. nucleatum.?Scanning?electron?microscopy?revealed?that?an?apparent?change?in?morphology?occurred?on?the?bacteria,?such?as?edge?coarsening?and?formation?of?cell?membrane?bubble?among?others.?Fluorescence?microscopy?showed?that?antimicrobial?peptide?can?lead?to?loss?of?integrity?of?bacterial?cell?membrane.?Conclusion???Antimicrobial?peptide?P9-0?effectively?restrains?common?pathogenic?bacteria?such?as?S. aureus?and?F. nucleatum?from?causing?oral?infection,?and?the?mechanism?of?action?is?possibly?membrane?destruction.