CAJ | 학술논문

人工合成苯巴比妥完全抗原并进行鉴定。对苯巴比妥结构进行修饰，合成带氨基基团的苯巴比妥半抗原，经重氮法与 BSA 和 OVA 制备苯巴比妥人工抗原。通过 TLC、质谱核磁共振对苯巴比妥半抗原进行鉴定，并采用紫外扫描对人工抗原进行结构鉴定，通过动物免疫对人工抗原进行生物学鉴定，使用 ELISA 对得到的抗血清进行鉴定分析。结果表明：苯巴比妥半抗原合成成功，苯巴比妥与 BSA，OVA 偶联成功，经计算其偶联比分别为231和10．91．动物免疫表明，免疫的5只小鼠的效价均在104以上，且4号小鼠的抗血清抗苯巴比妥 IC50为175．19 ng·mL－1。此实验成功合成了苯巴比妥人工抗原，为进一步制备苯巴比妥的特异性抗体与建立苯巴比妥免疫检测方法奠定了基础。
인공합성분파비타완전항원병진행감정。대분파비타결구진행수식，합성대안기기단적분파비타반항원，경중담법여 BSA 화 OVA 제비분파비타인공항원。통과 TLC、질보핵자공진대분파비타반항원진행감정，병채용자외소묘대인공항원진행결구감정，통과동물면역대인공항원진행생물학감정，사용 ELISA 대득도적항혈청진행감정분석。결과표명：분파비타반항원합성성공，분파비타여 BSA，OVA 우련성공，경계산기우련비분별위231화10．91．동물면역표명，면역적5지소서적효개균재104이상，차4호소서적항혈청항분파비타 IC50위175．19 ng·mL－1。차실험성공합성료분파비타인공항원，위진일보제비분파비타적특이성항체여건립분파비타면역검측방법전정료기출。
The current study aimed to synthesize the complete antigen of phenobarbital.Artificial phenobar-bital hapten with the active group amino group was designed and synthesized by chemical modification and linked to BSA and OVA through the diazotization method.Phenobarbital hapten was identified by TLC、MS.The structure of artificial phenobarbital antigen was identified by ultraviolet scanning.Moreover,the biological identification of artificial phenobarbital antigen was done by mice immunization.Polyclonal anti-serum was obtained,identified and analyzed by ELISA.The results showed that the synthesis of phenobar-bital hapten was successful.The phenobarbital was coupled with BSA and OVA,and the conjugation ratio was about 231 and 10.91.The animal immunization results showed that titer against phenobarbital of five mice were above 104 and IC50 of NO.4 mice was 175.19ng/mL.Artificial Phenobarbital antigen were synthesized and confirmed in the study.Therefore,it is possible to establish a base of the generation specif-ic antibody and development of immunoassay of phenobarbital.