南昌大学学报(理科版)
南昌大學學報(理科版)
남창대학학보(이과판)
JOURNAL OF NANCHANG UNIVERSITY(NATURAL SCIENCE)
2015年
1期
88-90,95
,共4页
姚闽%肖草茂%李玉云%伍锡栋
姚閩%肖草茂%李玉雲%伍錫棟
요민%초초무%리옥운%오석동
乌骨藤%皂苷%人宫颈腺癌
烏骨籐%皂苷%人宮頸腺癌
오골등%조감%인궁경선암
Marsdenia tenaccima%Glycosides%Human cervical adenocarcinoma
分离纯化乌骨藤提取物成分,通过建立荷人宫颈腺癌裸鼠模型,研究其抗肿瘤生物活性作用。采用硅胶色谱方法从乌骨藤提取物中分离纯化得到两个皂苷晶体,利用光谱方法进行了结构确定。通过裸鼠灌胃方法检测乌骨藤皂苷晶体Ⅰ、Ⅱ对荷人宫颈腺癌裸鼠肿瘤抑制作用。乌骨藤晶体Ⅰ、Ⅱ分别被鉴定为11α-O-顺芷酰-12β-O-乙酰通关藤苷元 B-3-β-O-D-葡萄吡喃糖基-(1→4)-6-去氧-3-O-甲基-D-阿洛吡喃糖基-(1→4)-D-夹竹桃吡喃糖苷(Ⅰ)、11α-O-顺芷酰-12β-O-乙酰通关藤苷元 B-3-β-O-D-葡萄吡喃糖基-(1→4)-β-O-D-葡萄吡喃糖基-(1→4)-6-去氧-3-O-甲基-D-阿洛吡喃糖基-(1→4)-D-夹竹桃吡喃糖苷(Ⅱ)。通过荷人宫颈腺癌裸鼠的肿瘤抑制实验,显示乌骨藤皂苷Ⅰ、Ⅱ对肿瘤生长具有明显抑制作用(P <0.01)。皂苷类成分具有抑制肿瘤生长功效,该类成分在乌骨藤植物中含量高,具有重要的开发研究价值。
分離純化烏骨籐提取物成分,通過建立荷人宮頸腺癌裸鼠模型,研究其抗腫瘤生物活性作用。採用硅膠色譜方法從烏骨籐提取物中分離純化得到兩箇皂苷晶體,利用光譜方法進行瞭結構確定。通過裸鼠灌胃方法檢測烏骨籐皂苷晶體Ⅰ、Ⅱ對荷人宮頸腺癌裸鼠腫瘤抑製作用。烏骨籐晶體Ⅰ、Ⅱ分彆被鑒定為11α-O-順芷酰-12β-O-乙酰通關籐苷元 B-3-β-O-D-葡萄吡喃糖基-(1→4)-6-去氧-3-O-甲基-D-阿洛吡喃糖基-(1→4)-D-夾竹桃吡喃糖苷(Ⅰ)、11α-O-順芷酰-12β-O-乙酰通關籐苷元 B-3-β-O-D-葡萄吡喃糖基-(1→4)-β-O-D-葡萄吡喃糖基-(1→4)-6-去氧-3-O-甲基-D-阿洛吡喃糖基-(1→4)-D-夾竹桃吡喃糖苷(Ⅱ)。通過荷人宮頸腺癌裸鼠的腫瘤抑製實驗,顯示烏骨籐皂苷Ⅰ、Ⅱ對腫瘤生長具有明顯抑製作用(P <0.01)。皂苷類成分具有抑製腫瘤生長功效,該類成分在烏骨籐植物中含量高,具有重要的開髮研究價值。
분리순화오골등제취물성분,통과건립하인궁경선암라서모형,연구기항종류생물활성작용。채용규효색보방법종오골등제취물중분리순화득도량개조감정체,이용광보방법진행료결구학정。통과라서관위방법검측오골등조감정체Ⅰ、Ⅱ대하인궁경선암라서종류억제작용。오골등정체Ⅰ、Ⅱ분별피감정위11α-O-순지선-12β-O-을선통관등감원 B-3-β-O-D-포도필남당기-(1→4)-6-거양-3-O-갑기-D-아락필남당기-(1→4)-D-협죽도필남당감(Ⅰ)、11α-O-순지선-12β-O-을선통관등감원 B-3-β-O-D-포도필남당기-(1→4)-β-O-D-포도필남당기-(1→4)-6-거양-3-O-갑기-D-아락필남당기-(1→4)-D-협죽도필남당감(Ⅱ)。통과하인궁경선암라서적종류억제실험,현시오골등조감Ⅰ、Ⅱ대종류생장구유명현억제작용(P <0.01)。조감류성분구유억제종류생장공효,해류성분재오골등식물중함량고,구유중요적개발연구개치。
The study aimed to separat and purify the saponin form Marsdenia tenacissima ,and researching further investigateon the antitumor biological activities through establishment of transplanted human cervi-cal cancer in nude mice model.The compounds were isolated by silica gel chromatography,which were then identified by the spectroscopy.The compounds Ⅰ,Ⅱ were detected with inhibitory effects on human cervi-cal cancer in nude mice through gavage methods.Two compounds were identified as 11-O-tigloxyl-12β-O-ethanoyl tenacigenin B 3-O-β-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1→4)-β-D-oleandropyranoside (Ⅰ),11α-O-tigloxyl-12β-O-ethanoyltenacigenin B-3-β-O-D-glucopyranosyl-(1→4)-β-O-D-glucopyranosyl-(1→4)-6-deoxy-3-O-methyl-β-D-allopyranosyl-(1 →4)-β-D-oleandropyranoside(II).They present obvious inhibitory effects after inhibitory experiment through human cervical cancer in nude mice. CONCLUSION:Saponins possessed to inhibit tumor growth,they were high content and important devel-opment value.