中华实验眼科杂志
中華實驗眼科雜誌
중화실험안과잡지
CHINESE JOURNAL OF EXPERIMENTAL OPHTHALMOLOGY
2015年
5期
436-439
,共4页
韩竹梅%张振永%张兴儒%柯梅青%李青松%项敏泓
韓竹梅%張振永%張興儒%柯梅青%李青鬆%項敏泓
한죽매%장진영%장흥유%가매청%리청송%항민홍
结膜疾病%成纤维细胞%Tenon囊%细胞培养%酶联免疫吸附测定法%结膜松弛症%抗炎剂
結膜疾病%成纖維細胞%Tenon囊%細胞培養%酶聯免疫吸附測定法%結膜鬆弛癥%抗炎劑
결막질병%성섬유세포%Tenon낭%세포배양%매련면역흡부측정법%결막송이증%항염제
Conjunctival diseases%Fibroblasts%Tenon capsule%Cells,cultured%Enzyme-linked immunosorbent assay%Conjunctivochalasis%Anti-inflammatory agents
背景 结膜松弛症是老年人中常见的眼表疾病,目前发病机制尚未明确,研究表明结膜松弛症常伴有局部炎症反应,炎性细胞因子在其发生和发展过程中发挥一定的作用. 目的 探讨穿透素3(PTX-3)和肿瘤坏死因子-α刺激基因6(TSG-6)在结膜松弛症患者结膜成纤维细胞中的表达. 方法 纳入2011年1月至2012年6月在上海中医药大学附属普陀医院确诊的结膜松弛症患者13例13眼、年龄相关性白内障患者16例16眼及翼状胬肉患者15例16眼,在相应的手术治疗过程中收集结膜组织标本进行成纤维细胞的原代培养和传代.选取3～6代对数生长期细胞用于实验,采用(ELISA)法检测并比较3种细胞培养液上清中PTX-3和TSG-6蛋白的质量浓度.结果 倒置显微镜下观察组织块透亮性差,呈暗黑色区域,周围有细胞溢出,传至第3代,为形态一致的纤维样细胞,大小均一,呈放射状排列,长梭形或两头尖的长条状,细胞质中有一个卵圆形的细胞核,周围有长短不等的细胞突起相互交联.结膜松弛症组患者成纤维细胞培养液上清中PTX-3的质量浓度为(2 182.33±738.68) pg/ml,明显高于年龄相关性白内障组的(738.32±335.00) pg/ml和翼状胬肉组的(981.37±416.04) pg/ml,3个组间总体差异有统计学意义(F=6.474,P=0.032);结膜松弛症组、翼状胬肉组和年龄相关性白内障组患者成纤维细胞培养液上清中的TSG-6的质量浓度分别为(59.10±6.52)、(53.99±11.16)和(35.01 ±5.33) pg/ml,3个组间总体比较差异有统计学意义(F=7.421,P=0.024),其中年龄相关性白内障组患者成纤维细胞培养液上清中TSG-6的质量浓度明显低于结膜松弛症组和翼状胬肉组,差异均有统计学意义(均P＜0.05),但结膜松弛症组与翼状胬肉组间TSG-6的质量浓度差异无统计学意义(P＞0.05).结论 炎症反应参与结膜松弛症的发生和发展过程,PTX-3、TSG-6在局部组织中的表达上调发挥一定作用.
揹景 結膜鬆弛癥是老年人中常見的眼錶疾病,目前髮病機製尚未明確,研究錶明結膜鬆弛癥常伴有跼部炎癥反應,炎性細胞因子在其髮生和髮展過程中髮揮一定的作用. 目的 探討穿透素3(PTX-3)和腫瘤壞死因子-α刺激基因6(TSG-6)在結膜鬆弛癥患者結膜成纖維細胞中的錶達. 方法 納入2011年1月至2012年6月在上海中醫藥大學附屬普陀醫院確診的結膜鬆弛癥患者13例13眼、年齡相關性白內障患者16例16眼及翼狀胬肉患者15例16眼,在相應的手術治療過程中收集結膜組織標本進行成纖維細胞的原代培養和傳代.選取3～6代對數生長期細胞用于實驗,採用(ELISA)法檢測併比較3種細胞培養液上清中PTX-3和TSG-6蛋白的質量濃度.結果 倒置顯微鏡下觀察組織塊透亮性差,呈暗黑色區域,週圍有細胞溢齣,傳至第3代,為形態一緻的纖維樣細胞,大小均一,呈放射狀排列,長梭形或兩頭尖的長條狀,細胞質中有一箇卵圓形的細胞覈,週圍有長短不等的細胞突起相互交聯.結膜鬆弛癥組患者成纖維細胞培養液上清中PTX-3的質量濃度為(2 182.33±738.68) pg/ml,明顯高于年齡相關性白內障組的(738.32±335.00) pg/ml和翼狀胬肉組的(981.37±416.04) pg/ml,3箇組間總體差異有統計學意義(F=6.474,P=0.032);結膜鬆弛癥組、翼狀胬肉組和年齡相關性白內障組患者成纖維細胞培養液上清中的TSG-6的質量濃度分彆為(59.10±6.52)、(53.99±11.16)和(35.01 ±5.33) pg/ml,3箇組間總體比較差異有統計學意義(F=7.421,P=0.024),其中年齡相關性白內障組患者成纖維細胞培養液上清中TSG-6的質量濃度明顯低于結膜鬆弛癥組和翼狀胬肉組,差異均有統計學意義(均P＜0.05),但結膜鬆弛癥組與翼狀胬肉組間TSG-6的質量濃度差異無統計學意義(P＞0.05).結論 炎癥反應參與結膜鬆弛癥的髮生和髮展過程,PTX-3、TSG-6在跼部組織中的錶達上調髮揮一定作用.
배경 결막송이증시노년인중상견적안표질병,목전발병궤제상미명학,연구표명결막송이증상반유국부염증반응,염성세포인자재기발생화발전과정중발휘일정적작용. 목적 탐토천투소3(PTX-3)화종류배사인자-α자격기인6(TSG-6)재결막송이증환자결막성섬유세포중적표체. 방법 납입2011년1월지2012년6월재상해중의약대학부속보타의원학진적결막송이증환자13례13안、년령상관성백내장환자16례16안급익상노육환자15례16안,재상응적수술치료과정중수집결막조직표본진행성섬유세포적원대배양화전대.선취3～6대대수생장기세포용우실험,채용(ELISA)법검측병비교3충세포배양액상청중PTX-3화TSG-6단백적질량농도.결과 도치현미경하관찰조직괴투량성차,정암흑색구역,주위유세포일출,전지제3대,위형태일치적섬유양세포,대소균일,정방사상배렬,장사형혹량두첨적장조상,세포질중유일개란원형적세포핵,주위유장단불등적세포돌기상호교련.결막송이증조환자성섬유세포배양액상청중PTX-3적질량농도위(2 182.33±738.68) pg/ml,명현고우년령상관성백내장조적(738.32±335.00) pg/ml화익상노육조적(981.37±416.04) pg/ml,3개조간총체차이유통계학의의(F=6.474,P=0.032);결막송이증조、익상노육조화년령상관성백내장조환자성섬유세포배양액상청중적TSG-6적질량농도분별위(59.10±6.52)、(53.99±11.16)화(35.01 ±5.33) pg/ml,3개조간총체비교차이유통계학의의(F=7.421,P=0.024),기중년령상관성백내장조환자성섬유세포배양액상청중TSG-6적질량농도명현저우결막송이증조화익상노육조,차이균유통계학의의(균P＜0.05),단결막송이증조여익상노육조간TSG-6적질량농도차이무통계학의의(P＞0.05).결론 염증반응삼여결막송이증적발생화발전과정,PTX-3、TSG-6재국부조직중적표체상조발휘일정작용.
Background Conjunctivochalasis is an age-related inflammatory ocular surface disease manifesting redundant,loose conjunctiva folds.Studies showed that conjunctivochalasis often accompanies with inflammatory response,indicating that a variety of inflammatory cytokines are involved in pathogenesis of conjunctivochalasis.Objective This study attempted to investigate the expressions of pentraxin-3 (PTX-3) and tumor necrosis factor-α stimulated gene-6 (TSG-6) proteins in fibroblasts of conjunctivochalasis.Methods The protocol was approved by Ethic Committee of Putuo Hospital affiliated Shanghai University of Traditional Chinese Medicine.Thirteen eyes of 13 patients with conjunctivochalasis,16 eyes of 16 patients with age-related cataract and 16 eyes of 15 patients with pterygium were included in Putuo Hospital Affiliated to Shanghai University of Traditional Medicine from January,2011 to June,2012.The samples of conjunctival tissue were collected in the patients during surgery under the informed consent,and fibroblasts were cultured and passaged by explant culture method.Content of PTX-3 and TSG-6 proteins in cell supernatant was detected by ELISA.Results Translucent poor,dark black area was around the cell overflow.Cultured cells of third generation showed long shuttle type,uniform size,radiated out in all directions,and oval nuclei was sited in the cytoplasm,surrounded by varying the length of cross-linking of cell processes.The concentrations of PTX-3 protein in cell supernatant were (2 182.33 ± 738.68) pg/ml in the conjunctivochalasis group,which were higher than (738.32±335.00) pg/ml in the age-related cataract group and (981.37±416.04)pg/ml in the pterygium group,showing a significant difference among the three groups (F =6.474,P=0.032).The contents of TSG-6 proteins in cell supernatant were (59.10±6.52) pg/ml,(53.99± 11.16) pg/ml and (35.01±5.33)pg/ml in the conjunctivochalasis group,pterygium group and age-related cataract group,with a significant difference among the three groups (F =7.421,P =0.024),and contents of TSG-6 proteins in the agerelated cataract group was lowest,however,no significant difference was found in the TSG-6 contents between the conjunctivochalasis group and the pterygium group (P＞0.05).Conclusions Inflammatory reaction participates in the pathogenesis and development of conjunctivochalasis,up-regulation of PTX-3 and TSG-6 expression in fibroblasts might partakes in the pathogenesis of conjunctivochalasis.
