CAJ | 학술논문

目的：观察通心络胶囊对糖尿病周围神经病变（DPN ）小鼠氧化应激的作用并探讨其机制。方法 KK／Upj-Ay小鼠分为模型组、通心络高、中、低剂量组，另设C57BL／6小鼠为对照组。灌胃给药12周，测定热痛觉阈值和运动神经传导速度（MNCV ）；比色法测定血液中超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px ）活性和丙二醛（MDA）含量；荧光定量PCR和Western blot测定坐骨神经血红素氧合酶-1（HO-1）、γ-谷氨酰半胱氨酸合成酶（γ-GCS）表达；Western blot 测定坐骨神经 p38 MAPK、p-p38 MAPK、JNK、p-JNK、ERK、p-ERK蛋白表达。结果与模型组比较，通心络组小鼠痛觉阈值升高，MNCV 明显增快（P＜0.05，P＜0.01）；SOD、GSH-Px活性明显上升，MDA含量上升（P＜0.01）；HO-1、γ-GCS mRNA和蛋白表达明显升高（P＜0.05， P＜0.01）；p-p38 MAPK 表达明显下降（P ＜0.05）。结论通心络胶囊可通过抑制p38 MAPK磷酸化水平而发挥其抗DPN小鼠氧化应激损伤的作用。
목적：관찰통심락효낭대당뇨병주위신경병변（DPN ）소서양화응격적작용병탐토기궤제。방법 KK／Upj-Ay소서분위모형조、통심락고、중、저제량조，령설C57BL／6소서위대조조。관위급약12주，측정열통각역치화운동신경전도속도（MNCV ）；비색법측정혈액중초양화물기화매（SOD）、곡광감태과양화물매（GSH-Px ）활성화병이철（MDA）함량；형광정량PCR화Western blot측정좌골신경혈홍소양합매-1（HO-1）、γ-곡안선반광안산합성매（γ-GCS）표체；Western blot 측정좌골신경 p38 MAPK、p-p38 MAPK、JNK、p-JNK、ERK、p-ERK단백표체。결과여모형조비교，통심락조소서통각역치승고，MNCV 명현증쾌（P＜0.05，P＜0.01）；SOD、GSH-Px활성명현상승，MDA함량상승（P＜0.01）；HO-1、γ-GCS mRNA화단백표체명현승고（P＜0.05， P＜0.01）；p-p38 MAPK 표체명현하강（P ＜0.05）。결론통심락효낭가통과억제p38 MAPK린산화수평이발휘기항DPN소서양화응격손상적작용。
Aim To investigate the effects of Tongxin-luo capsule on oxidative stress in diabetic peripheral neuropathy (DPN )mice and its mechanisms.Meth-ods KK/Upj-Ay mice were divided into model, Tongxinluo low-dose group, Tongxinluo middle-dose group and Tongxinluo high-dose group.C57BL/6 mice were selected as control group.Mice were given drugs intragastrically for 12 weeks.Paw withdrawal latency, motor nerve conduction velocity (MNCV)were detec-ted.Activity of superoxide dismutase (SOD),gluta-thione peroxidase (GSH-Px)and content of malondial-dehyde (MDA)in blood were detected by colorimetric method.The expression of heme oxygenase-1 (HO-1 ),γ-glutamyl cysteine synthetase (γ-GCS ) of sciatic nerve was examined by real time PCR and Western blot.The protein expression of p38 MAPK,p-p38 MAPK,JNK,p-JNK,ERK,p-ERK was examined by Western blot.Results Compared with model group, paw withdrawal latency was increased and MNCV was faster in Tongxinluo group (P <0.05 ,P <0.0 1 ). SOD and GSH-Px contents significantly increased, MDA content decreased (P <0.01 ).HO-1,γ-GCS mRNA and protein expression significantly increased (P<0.05,P <0.01 )in Tongxinluo group.p-p38 MAPK protein expression decreased in Tongxinluo group (P<0.05 ).Conclusion Tongxinluo can in-hibit oxidative stress in DPN of mice via suppressing the phosphorylation of p38 MAPK.