中国药理学通报
中國藥理學通報
중국약이학통보
CHINESE PHARMACOLOGICAL BULLETIN
2015年
5期
697-703
,共7页
乳腺癌%肿瘤微环境%间质%激光捕获显微切割%乳腺上皮细胞%质谱
乳腺癌%腫瘤微環境%間質%激光捕穫顯微切割%乳腺上皮細胞%質譜
유선암%종류미배경%간질%격광포획현미절할%유선상피세포%질보
breast cancer%tumor microenvironment%stroma%laser capture microdissection%mammary epi-thelium%mass spectrometry
目的：研究乳腺癌上皮细胞和间质间蛋白表达的差异。方法用改良HE染色法对临床侵润性导管内原位癌患者的石蜡包埋样品进行染色，用激光捕获显微切割技术（LCM）纯化分离癌变的乳腺上皮细胞和其周围间质后分别进行质谱分析。结果质谱总共检测了431种蛋白，上皮细胞和间质中分别检测到384和298种蛋白。251种共同表达的蛋白中，69种间质蛋白的含量比在上皮细胞中高，60种比在上皮细胞中低。所检测到蛋白的上皮细胞和间质定位及表达水平和它们在肿瘤发生、发展过程中的作用一致。结论在上皮细胞和间质中差异表达蛋白可以是疾病的筛选、诊断和预后判断的蛋白标志。
目的：研究乳腺癌上皮細胞和間質間蛋白錶達的差異。方法用改良HE染色法對臨床侵潤性導管內原位癌患者的石蠟包埋樣品進行染色，用激光捕穫顯微切割技術（LCM）純化分離癌變的乳腺上皮細胞和其週圍間質後分彆進行質譜分析。結果質譜總共檢測瞭431種蛋白，上皮細胞和間質中分彆檢測到384和298種蛋白。251種共同錶達的蛋白中，69種間質蛋白的含量比在上皮細胞中高，60種比在上皮細胞中低。所檢測到蛋白的上皮細胞和間質定位及錶達水平和它們在腫瘤髮生、髮展過程中的作用一緻。結論在上皮細胞和間質中差異錶達蛋白可以是疾病的篩選、診斷和預後判斷的蛋白標誌。
목적：연구유선암상피세포화간질간단백표체적차이。방법용개량HE염색법대림상침윤성도관내원위암환자적석사포매양품진행염색，용격광포획현미절할기술（LCM）순화분리암변적유선상피세포화기주위간질후분별진행질보분석。결과질보총공검측료431충단백，상피세포화간질중분별검측도384화298충단백。251충공동표체적단백중，69충간질단백적함량비재상피세포중고，60충비재상피세포중저。소검측도단백적상피세포화간질정위급표체수평화타문재종류발생、발전과정중적작용일치。결론재상피세포화간질중차이표체단백가이시질병적사선、진단화예후판단적단백표지。
Aim To study the difference of proteins expression of epithelial cells and stroma of breast canc-er patients.Methods Formalin-fixed paraffin-embed-ded tissue samples from invasive ductal carcionma pa-tients were stained by improved HE staining methods. And the neoplastic epithelium and stroma were micro-dissected using laser capture microdissection and ana-lysed by mass spectrometry.Results Total 43 1 differ-ent proteins were detected by mass spectrometry and there were respectively 384 and 298 different kinds of proteins identified in epithelial cells and stroma.A-mong them,25 1 proteins were commonly expressed in two samples,while the contents of 69 and 60 proteins in stroma were respectively higher or lower than the ones in epithelial cells.The expression level and local-ization of proteins identified in epithelial cells and stro-ma were associated with their roles in development and progression of tumor cells.Conclusions The differen-tial expression proteins between epithelial cells and stroma of invasive ductal carcionma patients may func-tion as biomarkers for breast cancer screenning,diag-nosis and prognosis.
