CAJ | 학술논문

目的：探讨乙型肝炎肝硬化患者血清HBV DNA水平与外周血HBV特异性细胞毒性 T 淋巴细胞（CTL）表面程序性死亡受体－1（PD－1）表达及肝功能的关系。方法：将109例HBV DNA阳性、HBeAg阳性和人白细胞抗原（HLA）－A2阳性的乙型肝炎肝硬化患者根据HBV DNA水平分为2组，甲组52例，HBV DNA 2～4 log10拷贝／mL，乙组57例，HBV DNA 5～7 log10拷贝／mL，比较2组患者的 HBV 特异性 CTL表面PD－1表达、HBV特异性CTL水平和肝功能的差异。结果：甲组HBV特异性CTL表面PD－1表达低于乙组（t ＝11．101，P ＜0．01），HBV特异性CTL 水平高于乙组（t ＝24．424，P ＜0．01），丙氨酸氨基转移酶低于乙组（t ＝2．652，P ＜0．01），血清白蛋白高于乙组（t ＝2．347，P ＜0．05）。Child－pugh分级，甲组C级低于乙组（χ2＝4．262，P ＜0．05）。结论：乙型肝炎肝硬化患者血清HBV DNA水平高者，HBV特异性CTL表面PD－1表达水平高，因此， HBV 特异性 CTL 水平低，进一步导致 HBV DNA 水平升高，导致肝功能损害较重， HBV DNA 水平低者， HBV 特异性 CTL 表面 PD－1表达水平低， HBV 特异性 CTL 水平高，预示抗病毒疗效相对较好。
목적：탐토을형간염간경화환자혈청HBV DNA수평여외주혈HBV특이성세포독성 T 림파세포（CTL）표면정서성사망수체－1（PD－1）표체급간공능적관계。방법：장109례HBV DNA양성、HBeAg양성화인백세포항원（HLA）－A2양성적을형간염간경화환자근거HBV DNA수평분위2조，갑조52례，HBV DNA 2～4 log10고패／mL，을조57례，HBV DNA 5～7 log10고패／mL，비교2조환자적 HBV 특이성 CTL표면PD－1표체、HBV특이성CTL수평화간공능적차이。결과：갑조HBV특이성CTL표면PD－1표체저우을조（t ＝11．101，P ＜0．01），HBV특이성CTL 수평고우을조（t ＝24．424，P ＜0．01），병안산안기전이매저우을조（t ＝2．652，P ＜0．01），혈청백단백고우을조（t ＝2．347，P ＜0．05）。Child－pugh분급，갑조C급저우을조（χ2＝4．262，P ＜0．05）。결론：을형간염간경화환자혈청HBV DNA수평고자，HBV특이성CTL표면PD－1표체수평고，인차， HBV 특이성 CTL 수평저，진일보도치 HBV DNA 수평승고，도치간공능손해교중， HBV DNA 수평저자， HBV 특이성 CTL 표면 PD－1표체수평저， HBV 특이성 CTL 수평고，예시항병독료효상대교호。
Objective To explore the relationship between serum HBV DNA level , expression of PD-1 on CTL and liver functions in patients with hepatitis B virus cirrhosis. Methods 109 HBV hepatic cirrhosis patients with , HBV DNA positive , HBeAg positive and HLA-A2 positive were divided into two groups according to their HBV DNA levels,52 cases in group A, HBV DNA 2-4 log10 copies/mL, 57cases in group B, HBV DNA 5-7 log10 copies/mL , differences of HBV specific CTL surface PD-1 expressions , HBV specific CTL levels and liver functions were compared between two groups. Results HBV specific CTL surface PD-1 expressions of group A were lower than that of group B (t = 11.101, P < 0.01), HBV specific CTL levels of group A were higher than that of group B (t = 24.424, P < 0.01), ALT of group A were lower than that of group B (t = 2.652, P < 0.01), ALB of group A were higher than that of group B (t = 2.347, P < 0.05). Child-pugh rating, grade C of group A was lower than that of group B (χ2= 4.262,P < 0.05). Conclusions HBV specific CTL surface PD-1 expression levels were higher in those hepatic cirrhosis patients with , high level of serum HBV DNA, and HBV specific CTL levels were lower, liver functions damage were much serious. So, lower HBV DNA levels , lower HBV specific CTL surface PD-1 expression levels and higher HBV specific CTL levels can product relatively good effect of anti-viral treatment.