CAJ | 학술논문

为寻找新型α-葡萄糖苷酶抑制（α-glucosidase inhibition，α-GI）活性化合物来源，针对前期研究发现的具有α-GI 活性的海绵附生微生物坚强芽孢杆菌（Bacillus firmus），对其发酵培养条件进行优化，并对其活性组分中主要成分进行初步分析．以α-GI 活性为主要检测对象，选出蛋白胨、酵母膏、NaCl 和 pH 值共4个因素进行正交优化试验．结合菌株生长情况和代谢产物产量指标，发现在 MB培养基的基础上，蛋白胨质量浓度3 g／dm3、酵母膏质量浓度3 g／dm3、NaCl 质量浓度19．5 g／dm3、pH 值为7．5的组合为最优培养条件．在优化后的培养条件下，规模化发酵该菌株，提取其代谢产物粗提物，经活性追踪下的二次色谱柱分离，获得一个高抑制活性组分，抑制率达82．4％．该组分经GC-MS 分析后发现，活性组分中主要成分为环二肽类及哌啶酮类物质．本研究结果为寻找新型α-GI 活性化合物提供了一个新的、可扩大生产的来源．
위심조신형α-포도당감매억제（α-glucosidase inhibition，α-GI）활성화합물래원，침대전기연구발현적구유α-GI 활성적해면부생미생물견강아포간균（Bacillus firmus），대기발효배양조건진행우화，병대기활성조분중주요성분진행초보분석．이α-GI 활성위주요검측대상，선출단백동、효모고、NaCl 화 pH 치공4개인소진행정교우화시험．결합균주생장정황화대사산물산량지표，발현재 MB배양기적기출상，단백동질량농도3 g／dm3、효모고질량농도3 g／dm3、NaCl 질량농도19．5 g／dm3、pH 치위7．5적조합위최우배양조건．재우화후적배양조건하，규모화발효해균주，제취기대사산물조제물，경활성추종하적이차색보주분리，획득일개고억제활성조분，억제솔체82．4％．해조분경GC-MS 분석후발현，활성조분중주요성분위배이태류급고정동류물질．본연구결과위심조신형α-GI 활성화합물제공료일개신적、가확대생산적래원．
In order to find new and active α-glucosidase inhibitors,the medium composition of a sponge-associated bacterium Bacillus firmus,which had been found possessing α-glucosidase inhibition (α-GI)activity in the previ-ous studies,was optimized through the orthogonal test.The active components in crude extracts of this strain were also isolated and analyzed.For an effective α-glucosidase inhibition,rapid cell growth (in terms of cell wet weight) and high crude extract production of the metabolites,an orthogonal test was conducted and it indicates that the com-bination of peptone 3 g/dm3 ,yeast extract 3 g/dm3 ,NaCl 19.5 g/dm3 and pH =7.5 is the optimal conditions based on the MB medium.The strain was fermented in large scale under the optimized conditions and the metabo-lites crude extract was extracted.After twice separation guided by α-GI activities on silicagel column,a sub-frac-tion with high activity of 82.4% against α-glucosidase was then obtained.The GC-MS analysis indicates that cyclic dipeptides and piperidones contributed major components in this active sub-fraction.Present study provided a new and applicable resource for the promising α-glucosidase inhibitors.