CAJ | 학술논문

目的：探讨PGE2对肿瘤坏死因子诱导的成骨细胞凋亡的抑制作用。方法本文以小鼠成骨样细胞MC3T3－E1为实验材料，通过MTT 法、Hoechst－PI双染检测凋亡、Caspase－3活性检测研究了PGE2对肿瘤坏死因子（ TNF－α）诱导的成骨细胞凋亡的调节作用。结果 Hoechst 33258－PI 双染检测可知，PGE2抑制肿瘤坏死因子TNF－α诱导的成骨细胞膜通透性的增强；以Caspase－3活性检测证实， PGE2可显著抑制TNF－α诱导的成骨细胞内的caspase－3活性。结论 PGE2对肿瘤坏死因子诱导的成骨细胞凋亡具有明显的抑制作用。
목적：탐토PGE2대종류배사인자유도적성골세포조망적억제작용。방법본문이소서성골양세포MC3T3－E1위실험재료，통과MTT 법、Hoechst－PI쌍염검측조망、Caspase－3활성검측연구료PGE2대종류배사인자（ TNF－α）유도적성골세포조망적조절작용。결과 Hoechst 33258－PI 쌍염검측가지，PGE2억제종류배사인자TNF－α유도적성골세포막통투성적증강；이Caspase－3활성검측증실， PGE2가현저억제TNF－α유도적성골세포내적caspase－3활성。결론 PGE2대종류배사인자유도적성골세포조망구유명현적억제작용。
Objective To investigate the inhibitory effect of PGE 2 on bone cell apoptosis induced by tumor necrosis factor.Methods In this paper, the regulatory role of PGE2 on TNFα-induced apoptosis taking the mouse osteoblast like MC3T3 -E1 cell as experimental material , through the MTT method, Hoechst -PI staining, Caspase -3 activity detection.Results Hoechst 33258 -PI staining experiment showed that PGE2 inhibited the membrane permeability induced by tumor necrosis factor alpha of bone cell .Further investigation indicated that the activity of caspase -3 was suppressed by PGE2.Conclusions PGE2 has significant inhibitory effect on tumor necrosis factor alpha induced apoptosis of osteoblast .