中国数字医学
中國數字醫學
중국수자의학
CHINA DIGITAL MEDICINE
2015年
5期
36-38
,共3页
连续切片%图像配准%图像处理%体视学
連續切片%圖像配準%圖像處理%體視學
련속절편%도상배준%도상처리%체시학
serial sections%image registration%image processing%stereology
肾脏在实现其生理功能的长期进化中，形成了空间排列精致的微细结构，而肾单位是实现上述功能的基本单元，因此对其发生与发育过程的研究非常重要。研究通过对C57小鼠胚龄17天、生后1天、3天、5天、7天和10天的肾脏取材，然后进行连续切片与图像采集，并配准；通过体视学方法对肾小体的面密度进行分析。结果显示，肾小体是随输尿管芽的发育由内向外逐步发生发育成熟的，至生后10天，早期肾小体结构在生后10天基本消失。
腎髒在實現其生理功能的長期進化中，形成瞭空間排列精緻的微細結構，而腎單位是實現上述功能的基本單元，因此對其髮生與髮育過程的研究非常重要。研究通過對C57小鼠胚齡17天、生後1天、3天、5天、7天和10天的腎髒取材，然後進行連續切片與圖像採集，併配準；通過體視學方法對腎小體的麵密度進行分析。結果顯示，腎小體是隨輸尿管芽的髮育由內嚮外逐步髮生髮育成熟的，至生後10天，早期腎小體結構在生後10天基本消失。
신장재실현기생리공능적장기진화중，형성료공간배렬정치적미세결구，이신단위시실현상술공능적기본단원，인차대기발생여발육과정적연구비상중요。연구통과대C57소서배령17천、생후1천、3천、5천、7천화10천적신장취재，연후진행련속절편여도상채집，병배준；통과체시학방법대신소체적면밀도진행분석。결과현시，신소체시수수뇨관아적발육유내향외축보발생발육성숙적，지생후10천，조기신소체결구재생후10천기본소실。
The physiological function and delicate spatial microstructures of kidneys are formed during its long-term evolution, and the basic unit of the function is nephron. Therefore, the research on kidney genesis and development is necessary. In this study, kidneys from C57 mice at Embryo 17 d and 1 d, 3 d, 5 d, 7 d 10 d after birth were taken, the tissues were serial sectioned, stained, image acquired, then aligned. The surface density of the renal corpuscle were analyze based on stereological method. The results showed that the renal corpuscle was genesis and developed with the development of ureteric bud from inside to outside gradually. The early morphological structure of renal structure was disappeared at the 10th day.
