CAJ | 학술논문

为了得到能高效降解纤维素的细菌，以纤维素粉为唯一碳源，从土壤中分离出一株能降解纤维素的细菌，通过分子生物学研究，鉴定其为假单胞菌属（Pseudomonas sp.）；并研究了温度和pH对其酶活的影响.结果表明，在pH 7.0、40℃时，其酶活最高，CMC酶活最高达31.36 U/mL，为进一步扩大纤维素降解菌种的筛选和应用范围奠定了基础.
위료득도능고효강해섬유소적세균，이섬유소분위유일탄원，종토양중분리출일주능강해섬유소적세균，통과분자생물학연구，감정기위가단포균속（Pseudomonas sp.）；병연구료온도화pH대기매활적영향.결과표명，재pH 7.0、40℃시，기매활최고，CMC매활최고체31.36 U/mL，위진일보확대섬유소강해균충적사선화응용범위전정료기출.
In order to get cellulose degrading bacteria,the selective culture method was utilized. As a result,a cellulose-degradation bacterium was separated from soil using the medium with cellulose powder as the sole carbon source. The bacterium was identified as Pseudomonas sp. by the method of molecular biology. The enzyme activity on CMC was higher than that on filter paper. The optimal conditions for the enzymatic reaction were 40℃and pH 7.0. This research makes a foundation of expanding the selection and application scope of cellulose-degradation strains for further screening.