CAJ | 학술논문

目的：探讨吗啡依赖大鼠觅药消退期边缘下区（infralimbic cortex, IL）遥测脑电活动的变化对觅药行为的影响及其机制。方法将SD大鼠随机分成模型组和对照组，在IL区实施脑立体定位电极埋植，模型组大鼠依次制成吗啡依赖和消退模型，应用无线遥测系统记录大鼠消退期IL区脑电活动的变化。结果模型组大鼠戒断1~2 d白箱停留时间明显长于吗啡注射前及对照组；消退训练后1~2 d，模型组大鼠白箱停留时间比戒断期明显缩短，与吗啡注射前及对照组相比，无明显差异。模型组与对照组比较，戒断2 d停留白箱时，IL区脑电β波显著增多，δ波显著减少；从黑箱向白箱穿梭时，δ波显著增多，α波和β波显著减少。消退2 d，模型组大鼠停留白箱时，IL区脑电比对照组θ波明显减少，与戒断期比较β波和θ波明显减少，δ波明显增多；从黑箱向白箱穿梭时，与对照组相比IL脑电无显著差异，但与戒断期比较，δ波明显减少，α波和β波明显增多。结论吗啡依赖大鼠觅药消退期IL区脑电活动发生异常变化，这种异常脑电的产生可能影响觅药动机的形成，进而抑制觅药行为的表达和维持。
목적：탐토마배의뢰대서멱약소퇴기변연하구（infralimbic cortex, IL）요측뇌전활동적변화대멱약행위적영향급기궤제。방법장SD대서수궤분성모형조화대조조，재IL구실시뇌입체정위전겁매식，모형조대서의차제성마배의뢰화소퇴모형，응용무선요측계통기록대서소퇴기IL구뇌전활동적변화。결과모형조대서계단1~2 d백상정류시간명현장우마배주사전급대조조；소퇴훈련후1~2 d，모형조대서백상정류시간비계단기명현축단，여마배주사전급대조조상비，무명현차이。모형조여대조조비교，계단2 d정류백상시，IL구뇌전β파현저증다，δ파현저감소；종흑상향백상천사시，δ파현저증다，α파화β파현저감소。소퇴2 d，모형조대서정류백상시，IL구뇌전비대조조θ파명현감소，여계단기비교β파화θ파명현감소，δ파명현증다；종흑상향백상천사시，여대조조상비IL뇌전무현저차이，단여계단기비교，δ파명현감소，α파화β파명현증다。결론마배의뢰대서멱약소퇴기IL구뇌전활동발생이상변화，저충이상뇌전적산생가능영향멱약동궤적형성，진이억제멱약행위적표체화유지。
Objective To investigate the changes of telemetry electrical activity in the infralimbic cortex (IL) of morphine-dependent rats with extinguished drug-seeking behavior. Methods SD rats were randomly divided into model group and control group and received operations of brain stereotaxic electrode embedding in the IL. The rats in the model group were induced to acquire morphine dependence and then received subsequent extinction training, and the changes of electrical activity in the IL were recorded with a physical wireless telemetry system. Results In rats with morphine dependence, the time staying in the white box was significantly longer on days 1 and 2 after withdrawal than that before morphine injection and that of the control rats, but was obviously reduced on days 1 and 2 after extinction training to the control level. Compared with the control group, the morphine-dependent rats on day 2 following withdrawal showed significantly increased β wave and decreased δ wave when they stayed in the white box but significantly increased δ wave and decreased α wave and β wave when they shuttled from the black to the white box. On day 2 of extinction, the model rats, when staying in the white box, showed significantly decreasedθwave compared with that of the control rats group but decreasedβwave andθwave and increasedδwave compared with those in the withdrawal period. When they shuttled from black to white box, the model rats showed decreased δ wave and increased α wave and β wave compared with those in the withdrawal period. Conclusion Morphine-dependent rats have abnormal changes of electrical activity in the IL in drug-seeking extinction to affect their drug-seeking motive and inhibit the expression and maintenance of drug-seeking behaviors.