CAJ | 학술논문

目的：探究氯喹对人鼻咽耐药细胞的逆转耐药作用及其机制。方法 MTT检测不同浓度的顺铂（2,4,8,16,32μmol · L-1）以及不同浓度的氯喹（5,10,20,40,80μmol · L-1）处理HNE1细胞，HNE1/DDP细胞48 h对细胞增殖的影响；q-PCR方法检测5，10μmol · L-1氯喹处理HNE1/DDP后的多药耐药基因MDR1 mRNA的表达；PI单染方法检测氯喹（10,20μmol · L-1）处理HNE1/DDP后细胞凋亡率；采用Western blot方法检测氯喹处理HNE1/DDP后的P-糖蛋白（P-glycoprotein, P-gp）的表达。结果 MTT结果显示，不同浓度的氯喹（5,10,20,40,80μmol · L-1）对细胞具有明显的抑制作用，并且呈浓度依赖性；q-PCR结果表明，氯喹作用细胞后能明显降低MDR1 mRNA水平；PI结果显示，氯喹作用细胞后，细胞的凋亡率明显增加；通过western blot实验表明，氯喹能明显降低MDR1和P-gp蛋白水平。结论氯喹能逆转人鼻咽癌细胞HNE1/DDP的耐药，其机制可能与下调MDR1 mRNA表达及抑制P-gp的功能与表达有关。
목적：탐구록규대인비인내약세포적역전내약작용급기궤제。방법 MTT검측불동농도적순박（2,4,8,16,32μmol · L-1）이급불동농도적록규（5,10,20,40,80μmol · L-1）처리HNE1세포，HNE1/DDP세포48 h대세포증식적영향；q-PCR방법검측5，10μmol · L-1록규처리HNE1/DDP후적다약내약기인MDR1 mRNA적표체；PI단염방법검측록규（10,20μmol · L-1）처리HNE1/DDP후세포조망솔；채용Western blot방법검측록규처리HNE1/DDP후적P-당단백（P-glycoprotein, P-gp）적표체。결과 MTT결과현시，불동농도적록규（5,10,20,40,80μmol · L-1）대세포구유명현적억제작용，병차정농도의뢰성；q-PCR결과표명，록규작용세포후능명현강저MDR1 mRNA수평；PI결과현시，록규작용세포후，세포적조망솔명현증가；통과western blot실험표명，록규능명현강저MDR1화P-gp단백수평。결론록규능역전인비인암세포HNE1/DDP적내약，기궤제가능여하조MDR1 mRNA표체급억제P-gp적공능여표체유관。
Objective To investigate the effect of chloroquine in reversing multidrug resistance (MDR) of HNE1/DDP cell line and explore the mechanism. Methods MTT assay was used to detect the cell viability of HNE1 and HNE1/DDP after exposure to different concentrations of DDP (2, 4, 8, 16 and 32μmol/L) and different concentrations of chloroquine (5, 10, 20, 40, and 80μmol/L). q-PCR was used to assess the expression of MDR1 mRNA and Western blotting was employed to detect P-glycoprotein (P-gp) expression in HNE1 and HNE1/DDP cells exposed to 5 and 10μmol/L chloroquine. The cell apoptosis rate of HNE1 and HNE1/DDP cells exposed to 10 and 20μmol/L chloroquine was determined by PI assay. Results Chloroquine exposure caused dose-dependent suppression of the proliferation in both HNE1 and HNE1/DDP cells, and significantly reversed multidrug resistance in HNE1/DDP cells. The expressions of MDR1 mRNA and P-gp protein were significantly lowered in the cells treated with chloroquine. Conclusion Chloroquine can reverse multidrug resistance in HNE1/DDP cells possibly through down-regulation of MDR1 and inhibition of P-gp protein.