广东微量元素科学
廣東微量元素科學
엄동미량원소과학
GUANGDONG TRACE ELEMENTS SCIENCE
2015年
3期
31-34
,共4页
PETIA法%胱抑素C%试剂开发
PETIA法%胱抑素C%試劑開髮
PETIA법%광억소C%시제개발
PETIA method%Cystatin C%reagent deve1opment
研制与评价一种镁离子增强的PETIA法检测血清胱抑素C试剂。在含有聚乙二醇6000和镁离子的pH 7.6的Tris-HC1缓冲液中，胱抑素C与包被有胱抑素C抗体的纳米胶乳微球反应并形成一定浊度，该浊度与样品中胱抑素C浓度成正比。该法线性范围为0~15.00 mg/L，批内和批间精密度（ CV）分别为0.54%~1.81%和0.72%~2.23%，平均回收率为99.9%，该法（ y）与参考方法（x）具有良好的相关性，线性回归方程和相关系数分别为 y =0.9984 x +0.026，r =0.9984。所述的试剂测定血清胱抑素C，具有操作简便、线性范围宽、灵敏度高的优点，具有推广应用价值。
研製與評價一種鎂離子增彊的PETIA法檢測血清胱抑素C試劑。在含有聚乙二醇6000和鎂離子的pH 7.6的Tris-HC1緩遲液中，胱抑素C與包被有胱抑素C抗體的納米膠乳微毬反應併形成一定濁度，該濁度與樣品中胱抑素C濃度成正比。該法線性範圍為0~15.00 mg/L，批內和批間精密度（ CV）分彆為0.54%~1.81%和0.72%~2.23%，平均迴收率為99.9%，該法（ y）與參攷方法（x）具有良好的相關性，線性迴歸方程和相關繫數分彆為 y =0.9984 x +0.026，r =0.9984。所述的試劑測定血清胱抑素C，具有操作簡便、線性範圍寬、靈敏度高的優點，具有推廣應用價值。
연제여평개일충미리자증강적PETIA법검측혈청광억소C시제。재함유취을이순6000화미리자적pH 7.6적Tris-HC1완충액중，광억소C여포피유광억소C항체적납미효유미구반응병형성일정탁도，해탁도여양품중광억소C농도성정비。해법선성범위위0~15.00 mg/L，비내화비간정밀도（ CV）분별위0.54%~1.81%화0.72%~2.23%，평균회수솔위99.9%，해법（ y）여삼고방법（x）구유량호적상관성，선성회귀방정화상관계수분별위 y =0.9984 x +0.026，r =0.9984。소술적시제측정혈청광억소C，구유조작간편、선성범위관、령민도고적우점，구유추엄응용개치。
Deve1opment and eva1uation of one magnesium ion enhanced PETIA serum Cystatin C assay reagent. The ana1ysis was based on the fact that the Cystatin C react to the Cystatin C antibody and then antigen-antibody Comp1ex was produced,the turbidity of antigen-antibody comp1ex was direct ratio to the concentration of serum Cystatin C. The method was 1iner in the range of 0~15. 00 mg/L,the within run and between run CV was 0. 54% ~ 1. 81% and 0. 72% ~ 2. 23%, the average recovery was 99. 9%. Comparing this methods( y) with refence method( x),we obtained the regression equations:y=0. 998 4 x +0. 026,r =0. 998 4. This method was simp1e,sensitive and suitab1e for c1inica1 app1ication.
