CAJ | 학술논문

目的：旨在验证灵芝酸A对海马神经元异常放电的影响。方法分离24h新生Wistar大鼠原代海马神经元。正常对照组：将培养9d的海马神经元全液换成正常细胞外液处理3h，然后恢复正常培养24h；无Mg2＋诱导组、灵芝酸A组、紫杉醇组、γ－氨基丁酸组、丙戊酸钠组分别将培养9d的海马神经元全液换成无镁细胞外液或添加相应的50μg／mL的灵芝酸A、50μmol／L的紫杉醇、100μmol／L的γ－氨基丁酸、100mg／L的丙戊酸钠混悬液处理3h，然后恢复正常培养，MTT法检测细胞存活率；分光光度计检测超氧化物歧化酶活性；流式细胞仪检测细胞线粒体膜电位的变化。结果50μg／mL灵芝酸A可提高海马神经元细胞存活率；能升高癫痫样海马神经元细胞SOD活性、升高线粒体膜电位。结论灵芝酸A可通过抑制细胞氧化损伤、抑制凋亡而保护异常放电的海马神经元。
목적：지재험증령지산A대해마신경원이상방전적영향。방법분리24h신생Wistar대서원대해마신경원。정상대조조：장배양9d적해마신경원전액환성정상세포외액처리3h，연후회복정상배양24h；무Mg2＋유도조、령지산A조、자삼순조、γ－안기정산조、병무산납조분별장배양9d적해마신경원전액환성무미세포외액혹첨가상응적50μg／mL적령지산A、50μmol／L적자삼순、100μmol／L적γ－안기정산、100mg／L적병무산납혼현액처리3h，연후회복정상배양，MTT법검측세포존활솔；분광광도계검측초양화물기화매활성；류식세포의검측세포선립체막전위적변화。결과50μg／mL령지산A가제고해마신경원세포존활솔；능승고전간양해마신경원세포SOD활성、승고선립체막전위。결론령지산A가통과억제세포양화손상、억제조망이보호이상방전적해마신경원。
Objective To isolate rat primary hippocampal neurons , detect the impact of Ganoderma acid A on the epileptic cell mitochondrial membrane potential and superoxide dismutase in ouder to investigate the antiepileptic mechanism of Ganoderma acid A , provide clinical antiepileptic drugs theory basis.Methods 24h newborn Wistar rat primary hippocampal neurons rere isolated , and then the experimental groups were divided into normal control group: the 9th days cultured hippocampal neurons in complete medium were treated with normal extracellular fluid for 3 hours, and recovered complete medium for 24h;no Mg2+induction group , Ganoderma acid group A , paclitaxel ,γ-aminobutyric acid group , sodium valproate group were treated with Mg 2+-free extracellular fluid or ad-ditional added 50μg /mL Ganoderma acid A, 50μmol /L paclitaxel, 100μmol /L γ-aminobutyric acid, 100mg /L sodium val-proate suspensions for 3h, and recovered complete medium for 24h;We detect the changes of the mitochondrial membrane potential by flow cytometry, superoxide dismutase activity by spectrophotometer , to explore the pathogenesis of epilepsy and the antiepileptic mecha-nism of Ganoderma acid A.Results We successfully isolated rat primary hippocampal neurons;Ganoderma acid A at 50μg /mL con-centration were nontoxic to hippocampal neurons;Ganoderma acid A increased the activity of SOD in hippocampal neurons , Ganoderma acid A elevated the epileptic mitochondrial membrane potential.Conclusion Ganoderma acid A has anti -epileptic effect by antioxi-dant and inhibiting apoptosis.