CAJ | 학술논문

现已证明血清／血浆 microRNAs（miRNAs）表达谱的变化与多种疾病的发生、发展相关。血清／血浆miRNAs分子稳定，不易降解，且标本采集创伤小、便捷，非常适合应用于临床实验室检测。因此miRNAs极有可能成为未来疾病诊断和预后评估的分子标志物乃至治疗靶标。实时荧光定量 PCR 检测法（real-time quantitative polymerase chain reaction， RT-qPCR）操作简便，需要的 RNA 量较少，灵敏度极高，能在短时间内获得结果，并可同时检测多种miRNAs 表达，有良好的应用前景。本文将详细探讨现阶段用于血清／血浆miRNAs 定量检测的RT-qPCR方法，以及总结血清／血浆miRNAs 定量检测应用于临床检验应注意的问题。
현이증명혈청／혈장 microRNAs（miRNAs）표체보적변화여다충질병적발생、발전상관。혈청／혈장miRNAs분자은정，불역강해，차표본채집창상소、편첩，비상괄합응용우림상실험실검측。인차miRNAs겁유가능성위미래질병진단화예후평고적분자표지물내지치료파표。실시형광정량 PCR 검측법（real-time quantitative polymerase chain reaction， RT-qPCR）조작간편，수요적 RNA 량교소，령민도겁고，능재단시간내획득결과，병가동시검측다충miRNAs 표체，유량호적응용전경。본문장상세탐토현계단용우혈청／혈장miRNAs 정량검측적RT-qPCR방법，이급총결혈청／혈장miRNAs 정량검측응용우림상검험응주의적문제。
It has been proved that the changes of serum/plasma microRNAs(miRNAs) expression relates to the occurrence and development of various diseases. The molecular of serum/plasma miRNAs are stable and hard to degrade. It is easy to obtain blood specimen from small wound and suitable to detect for clinical laboratory. Therefore, miRNAs are most likely to become a potential biomarker for disease diagnosis and prognosis evaluation, even the target spot of treatment. As is easy to operate with few RNA, and with high sensitivity to detect a variety of miRNAs expression in a short time, real time fluorescence quantitative PCR (RT-qPCR) detection method has a good application prospect. In this paper, a review on the detection methods of serum/plasma miRNAs with RT-qPCR at the present stage will be discussed, and the counter-measures on the clinical application of serum/plasma miRNAs quantitative detection will be summarized.