CAJ | 학술논문

目的：采用高效液相色谱法(HPLC)测定天麻成分对甲氧基苄醇在大鼠血液中的药代动力学参数。方法采用灌胃和静脉注射2种方式给药,HPLC 测定不同时间大鼠血液中天麻成分对甲氧基苄醇的浓度,DAS3.0软件计算药代动力学参数。结果大鼠血液中对甲氧基苄醇在0.63～321.17μg/mL浓度范围内线性关系良好,r2=0.9945；日内、日间精密度、绝对回收率和稳定性均在规定范围内。结论本方法操作简便,能较准确地测出天麻成分对甲氧基苄醇在大鼠血液中的药代动力学参数。
목적：채용고효액상색보법(HPLC)측정천마성분대갑양기변순재대서혈액중적약대동역학삼수。방법채용관위화정맥주사2충방식급약,HPLC 측정불동시간대서혈액중천마성분대갑양기변순적농도,DAS3.0연건계산약대동역학삼수。결과대서혈액중대갑양기변순재0.63～321.17μg/mL농도범위내선성관계량호,r2=0.9945；일내、일간정밀도、절대회수솔화은정성균재규정범위내。결론본방법조작간편,능교준학지측출천마성분대갑양기변순재대서혈액중적약대동역학삼수。
Objective To determine the pharmacokinetic parameters of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats by HPLC. Methods Gavage and intravenous injection were employed for administration. HPLC was used to determine the concentrations of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats in different time points. The pharmacokinetic parameters were computed by DAS3.0. Results The linear range of P-methoxybenzyl alcohol in plasma was 0.63-321.17 μg/mL, r 2=0.994 5. Intra-day accuracy, inter-day accuracy, absolute recovery and stability were in specified range. Conclusion The method is simple and accurate for the determination of the pharmacokinetic parameters of P-methoxybenzyl alcohol from Gastrodiae Rhizoma in plasma of rats.