CAJ | 학술논문

目的：探讨重症急性胰腺炎大鼠舌组织水通道蛋白3(AQP3)的表达及大黄素的调节作用。方法采用5%牛磺胆酸钠逆行胆胰管注入制作大鼠急性重症胰腺炎模型。将SD雄性大鼠随机分成假手术组、模型组、大黄素组,大黄素组于造模后每日予大黄素20 mg/kg灌胃,模型组及假手术组予等量生理盐水灌胃。造模后每日观察大鼠精神状态、厚腻苔出现情况及存活率；造模第5日后各组随机选取8只大鼠处死,留取标本,采用Western blot和实时定量PCR检测舌组织AQP3蛋白及基因表达。结果与模型组比较,大黄素组大鼠死亡率、厚腻苔发生率降低及厚腻苔发生明显减少,AQP3蛋白及基因表达明显降低(P＜0.05)。至实验第5日,模型组存活11只大鼠,出现厚腻苔5只；与假手术组比较,模型组大鼠舌组织AQP3蛋白及基因表达均明显升高(P＜0.05)。结论大黄素通过下调AQP3蛋白及基因表达,改善重症急性胰腺炎大鼠症状,减少厚腻苔的发生。
목적：탐토중증급성이선염대서설조직수통도단백3(AQP3)적표체급대황소적조절작용。방법채용5%우광담산납역행담이관주입제작대서급성중증이선염모형。장SD웅성대서수궤분성가수술조、모형조、대황소조,대황소조우조모후매일여대황소20 mg/kg관위,모형조급가수술조여등량생리염수관위。조모후매일관찰대서정신상태、후니태출현정황급존활솔；조모제5일후각조수궤선취8지대서처사,류취표본,채용Western blot화실시정량PCR검측설조직AQP3단백급기인표체。결과여모형조비교,대황소조대서사망솔、후니태발생솔강저급후니태발생명현감소,AQP3단백급기인표체명현강저(P＜0.05)。지실험제5일,모형조존활11지대서,출현후니태5지；여가수술조비교,모형조대서설조직AQP3단백급기인표체균명현승고(P＜0.05)。결론대황소통과하조AQP3단백급기인표체,개선중증급성이선염대서증상,감소후니태적발생。
Objective To investigate the expression of aquaporin-3 (AQP3) in tongue tissues of rats with experimental severe acute pancreatitis (SAP) and regulating effects of emodin. Methods Rat models with SAP were established by injecting 5% sodium taurocholate retrogradely into gallbladder and pancreas. SD male rats were randomly divided into sham-operation group, model group, and emodin group. After model establishment, rats in the emodin group received gavage with emodin 20 mg/kg each day, while rats in the model group and sham-operation group received gavage with normal saline. The mental state, thick greasy tongue fur and mortality of rats were observed every day after model establishment, and 5 days later, protein and genetic expression of AQP3 were detected by Western blot and RT-PCR. Results Compared with the model group, the mortality and the thick greasy tongue fur significantly decreased, the protein and genetic expression of AQP3 significantly decreased in the emodin group (P<0.05). On the 5th day, 11 rats in the model group survived, and 5 rats had thick greasy tongue fur. Compared with the sham-operation group, the protein and genetic expression of AQP3 in the model group were higher (P<0.05). Conclusion Emodin can improve the severity of SAP and decrease the incidence of thick greasy tongue fur significantly by reducing the protein and genetic expression of AQP3.